LOCUS       sequence-211539-       10028 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Chondrocyte specific inducible Cre.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10028)
  TITLE     Cre-inducible plasmids
REFERENCE   2  (bases 1 to 10028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Col2a1:TFPnls-mErt-Cre-Ert
FEATURES             Location/Qualifiers
     source          1..10028
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(31..459)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(141..162)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     353..372
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     586..608
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     600..617
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     601..617
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     650..669
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        650..668
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(683..694,695..882)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     intron          2985..3117
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     3100..3119
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     3214..3233
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      3281..3290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3287..3994
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     CDS             4019..4039
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4043..4063
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4067..4087
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4109..4162
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5117..6139
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="NLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLLS
                     VCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLPR
                     PSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFLG
                     IAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVERW
                     ISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQRY
                     LAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLEDG
                     D"
     CDS             6152..7087
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             6200..7093
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     primer_bind     complement(7177..7196)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7242..7297
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7296..7315)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     misc_feature    join(7642..7779,7780..7791)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(7818..7838)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7818..7836)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7880..7896)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7880..7896)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7893..7915)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7904..7920
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7928..7934,7935..7952,7953..7958))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7973..7994
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8111..8128)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8282..8870)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8362..8381)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9041..9832,9833..9901))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9664..9683
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9902..10006)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cctaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
       61 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
      121 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
      181 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
      241 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
      301 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
      361 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
      421 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc
      481 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
      541 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
      601 gtaaaacgac ggccagtgag cgcgtaggga taacagggta atgcgcgcgt aatacgactc
      661 actatagggc gaattgggta cccagaggtg taaagtactt gagtaatttt acttgattac
      721 tgtacttaag tattattttt ggggattttt actttacttg agtacaatta aaaatcaata
      781 cttttacttt tacttaatta cattttttta gaaaaaaaag tactttttac tccttacaat
      841 tttatttaca gtcaaaaagt acttattttt tggagatcac ttgggcccgg ctttcctgca
      901 agctcactgt agaaaagaga cctgctttaa caaattgcag tttttttcct gcctctctct
      961 aaatggtagc actttttttt ttgacagagg aatgagagag tttaggtgag gggggcgaag
     1021 gagggaagag agagagcagg agggtttggg acagtgggag ggaggcagaa ggggacagaa
     1081 agtggcaagc aagggaaagg gtccctgcaa gaaaggaggg gtttggtggg atggggagag
     1141 ggcagagagg agtggagggg ctcagatttc agctctgctg ggggcagggg aggactaaga
     1201 tgtgggcata tacgttgact tcaacccaca caggagtata acttcagtac cttggcccag
     1261 agcagttagt actaagtttc tgctggggcc tggtactgga ttctccttct tttcccaaga
     1321 aatcggctgc atgaagacaa gataactatt ggagacagcc atcctccttc tccccttctg
     1381 agctcgagaa aaatgtgcaa cttgctgggg caggtcccaa tggttagaga aaaaaataaa
     1441 aattccttta cgagtccttc acatactaca gactgtaaat ccattgcact gctagtatgg
     1501 aaaaggcatc tttttctgaa tatttgccag gttttttttt attggaatac ctattctttt
     1561 ccataaaacg ttcttaatct gaactgcaca tctgtagaaa ggggaatatg tgtgaaatgc
     1621 tcaagtagca ggctggaatc tctctcagct gatctactgt ctgtatagtt tccagtcaaa
     1681 gattgctttt catagcttaa atcagggctt tcagagggtt ctgggagttg tagttttaag
     1741 atagctgaaa agttacagcc ccctgcctta aatcatatac ttgctaaata catagtttac
     1801 cattaatacg attactggaa aatagacatg cttacctatg gcctttgaac tgttgttgaa
     1861 ctacaatccc caccagtcct tggctgtcga ggaatgctgg gagttgtagt ttattagcta
     1921 gaggacagaa ggttagacat ctctgatgca gaaaatagat tttgggtggc acctgtcaac
     1981 ttgagggttt gcatgttata taattccccc ttgaggagaa agcaataata aaggaaaaca
     2041 tactcatatg ggaattctat aaactcatag aggaaacctt ataagtatgt tattgttgaa
     2101 gactgctctg ggatatgagg aaatgctcaa aactttttaa tgtacgcata ttaagtctag
     2161 aggtccatcc gctgtactta taaaggtgat taaaatggaa gggccttgct taaaggcttc
     2221 aattcagtat tgtgatggtt tatttgcact gggaggattg gcagcaatgg tttccagatg
     2281 gagttgaaat gctgcccatg tttatttaaa ctggcccctg acagtgaaat gtgaattgag
     2341 acctctgtgt attttacaaa aaggcccatt cattaagggt cctctgggct acccaagcat
     2401 gggccatgga cccaacggga gtgatgcagg gtctaatcaa gatcactgct tgggtcacaa
     2461 tggattaata tgacatcctt tgttatctaa tagcctttat taacagaatc tcatcctacc
     2521 tttcaacaaa tgtgagaact agttggtatg taccattctt cccagtgttt gatcttagca
     2581 aacattcttg ataagtacag atttttcatc cagtagctgc attttattca attatgctcc
     2641 tttttagcct ttactatttc ctattaattt gctttatgag aaacagtgaa gacaccttag
     2701 ggagtctaat ttcaaaagca gattctctag cttttaatga cctcagggag actgttatta
     2761 actattcacc atagttcatg ttgtaatatt atactacaac ttcctaaact ctcagccaat
     2821 atatactgga tgaatgcccc cagctactca caccctcctt acaagactcc ctgcgctagc
     2881 tttacccaga tcacctttgc cacgcgtgaa ttcgctgagg gcgtttaaaa tcctcagcaa
     2941 ttcgaatttt aagctgcaga agttggtcgt gaggcactgg gcaggtaagt atcaaggtta
     3001 caagacaggt ttaaggagac caatagaaac tgggcttgtc gagacagaga agactcttgc
     3061 gtttctgata ggcacctatt ggtcttactg acatccactt tgcctttctc tccacaggtg
     3121 tccactccca gttcaattac agctcttaaa attccgccac tagagcctct gctaaccatg
     3181 ttcatgcctt cttctctttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc
     3241 atcattttgg caaagaatta ttccgctagc gctaccggtc gccaccatgg tgagcaaggg
     3301 cgaggagacc acaatgggcg taatcaagcc cgacatgaag atcaagctga agatggaggg
     3361 caacgtgaat ggccacgcct tcgtgatcga gggcgagggc gagggcaagc cctacgacgg
     3421 caccaacacc atcaacctgg aggtgaagga gggagccccc ctgcccttct cctacgacat
     3481 tctgaccacc gcgttcgcct acggcaacag ggccttcacc aagtaccccg acgacatccc
     3541 caactacttc aagcagtcct tccccgaggg ctactcttgg gagcgcacca tgaccttcga
     3601 ggacaagggc atcgtgaagg tgaagtccga catctccatg gaggaggact ccttcatcta
     3661 cgagatacac ctcaagggcg agaacttccc ccccaacggc cccgtgatgc agaagaagac
     3721 caccggctgg gacgcctcca ccgagaggat gtacgtgcgc gacggcgtgc tgaagggcga
     3781 cgtcaagcac aagctgctgc tggagggcgg cggccaccac cgcgttgact tcaagaccat
     3841 ctacagggcc aagaaggcgg tgaagctgcc cgactatcac tttgtggacc accgcatcga
     3901 gatcctgaac cacgacaagg actacaacaa ggtgaccgtt tacgagagcg ccgtggcccg
     3961 caattccacc gacggcatgg acgagctgta caagtccgga ctcagatctc gagctgatcc
     4021 aaaaaagaag agaaaggtag atccaaaaaa gaagagaaag gtagatccaa aaaagaagag
     4081 aaaggtagga tccaccggat ctagacccga gggcagagga agtcttctaa catgcggtga
     4141 cgtggaggag aatcccggcc cactagtcat ggccggcgat atgagagccg ctaacctgtg
     4201 gccctcccca ctgatgatca agagaagcaa gaagaactcc ctggccctga gcctgaccgc
     4261 tgatcagatg gtgtccgccc tgctggacgc cgagccccct atcctgtaca gcgagtacga
     4321 ccccaccagg cccttcagcg aggccagcat gatgggactg ctgacaaacc tggccgatcg
     4381 cgaactggtg catatgatca attgggccaa gcgcgtgccc ggattcgtgg acctgacact
     4441 gcacgaccag gtgcacctgc tggaatgcgc ttggctggaa attctgatga tcggcctcgt
     4501 gtggcggagc atggaacatc ctgtgaagct gctgttcgcc cccaatctgc tgctggaccg
     4561 caaccagggc aaatgcgtgg aagggatggt cgaaatcttt gatatgctgc tcgccaccag
     4621 cagcaggttc agaatgatga acctccaggg cgaagagttc gtctgcctga agtccatcat
     4681 cctgctgaac agcggcgtgt acaccttcct gtcctctacc ctgaagtccc tcgaggaaaa
     4741 ggatcacatc cacagggtgc tggataagat caccgatacc ctgatccatc tcatggctaa
     4801 ggccggactc acactccagc agcagcatca gagactcgct cagctgctgc tgatcctgag
     4861 ccacatccgc cacatgtcca acaagggaat ggaacacctc tactctatga agtgtaaaaa
     4921 cgtcgtcccc ctgtacgatc tgctgctcga ggccgccgat gcccacagac tgcacgcccc
     4981 tacaagcaga ggcggcgcta gcgtggaaga gacagaccag tcccacctgg ccaccgccgg
     5041 cagcacaagc agccacagcc tgcagaagta ctatatcacc ggcgaggccg agggcttccc
     5101 cgctacagct gtcgacaatt tactgaccgt acaccaaaat ttgcctgcat taccggtcga
     5161 tgcaacgagt gatgaggttc gcaagaacct gatggacatg ttcagggatc gccaggcgtt
     5221 ttctgagcat acctggaaaa tgcttctgtc cgtttgccgg tcgtgggcgg catggtgcaa
     5281 gttgaataac cggaaatggt ttcccgcaga acctgaagat gttcgcgatt atcttctata
     5341 tcttcaggcg cgcggtctgg cagtaaaaac tatccagcaa catttgggcc agctaaacat
     5401 gcttcatcgt cggtccgggc tgccacgacc aagtgacagc aatgctgttt cactggttat
     5461 gcggcggatc cgaaaagaaa acgttgatgc cggtgaacgt gcaaaacagg ctctagcgtt
     5521 cgaacgcact gatttcgacc aggttcgttc actcatggaa aatagcgatc gctgccagga
     5581 tatacgtaat ctggcatttc tggggattgc ttataacacc ctgttacgta tagccgaaat
     5641 tgccaggatc agggttaaag atatctcacg tactgacggt gggagaatgt taatccatat
     5701 tggcagaacg aaaacgctgg ttagcaccgc aggtgtagag aaggcactta gcctgggggt
     5761 aactaaactg gtcgagcgat ggatttccgt ctctggtgta gctgatgatc cgaataacta
     5821 cctgttttgc cgggtcagaa aaaatggtgt tgccgcgcca tctgccacca gccagctatc
     5881 aactcgcgcc ctggaaggga tttttgaagc aactcatcga ttgatttacg gcgctaagga
     5941 tgactccggt cagagatacc tggcctggtc tggacacagt gcccgtgtcg gagccgcgcg
     6001 agatatggcc cgcgctggag tttcaatacc ggagatcatg caagctggtg gctggaccaa
     6061 tgtaaatatt gtcatgaact atatccgtaa cctggatagt gaaacagggg caatggtgcg
     6121 cctgctggaa gatggcgatc tcgagccatc tgctggagac atgagagctg ccaacctttg
     6181 gccaagcccg ctcatgatca aacgctctaa gaagaacagc ctggccttgt ccctgacggc
     6241 cgaccagatg gtcagtgcct tgttggatgc tgagcccccc atactctatt ccgagtatga
     6301 tcctaccaga cccttcagtg aagcttcgat gatgggctta ctgaccaacc tggcagacag
     6361 ggagctggtt cacatgatca actgggcgaa gagggtgcca ggctttgtgg atttgaccct
     6421 ccatgatcag gtccaccttc tagaatgtgc ctggctagag atcctgatga ttggtctcgt
     6481 ctggcgctcc atggagcacc cagtgaagct actgtttgct cctaacttgc tcttggacag
     6541 gaaccaggga aaatgtgtag agggcatggt ggagatcttc gacatgctgc tggctacatc
     6601 atctcggttc cgcatgatga atctgcaggg agaggagttt gtgtgcctca aatctattat
     6661 tttgcttaat tctggagtgt acacatttct gtccagcacc ctgaagtctc tggaagagaa
     6721 ggaccatatc caccgagtcc tggacaagat cacagacact ttgatccacc tgatggccaa
     6781 ggcaggcctg accctgcagc agcagcacca gcggctggcc cagctcctcc tcatcctctc
     6841 ccacatcagg cacatgagta acaaaggcat ggagcatctg tacagcatga agtgcaagaa
     6901 cgtggtgccc ctctatgacc tgctgctgga ggcggcggac gcccaccgcc tacatgcgcc
     6961 cactagccgt ggaggggcat ccgtggagga gacggaccaa agccacttgg ccactgcggg
     7021 ctctacttca tcgcattcct tgcaaaagta ttacatcacg ggggaggcag agggtttccc
     7081 tgccacagct taagatatca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg
     7141 tgtggccaat gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta
     7201 tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt
     7261 cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc
     7321 aaatcattta aaacatcaga atgagcattt ggtttagagt ttggcaacat atgcccatat
     7381 gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc
     7441 tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt
     7501 ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc
     7561 cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc tcttatgaag
     7621 atccctcgac cagcggccgc taatactcaa gtacaatttt aatggagtac ttttttactt
     7681 ttactcaagt aagattctag ccagatactt ttacttttaa ttgagtaaaa ttttccctaa
     7741 gtacttgtac tttcacttga gtaaaatttt tgagtacttt ttacacctct gccgcggtgg
     7801 agctccagct tttgttccct ttagtgaggg ttaattgcgc gcattaccct gttatcccta
     7861 cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     7921 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     7981 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     8041 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     8101 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     8161 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     8221 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     8281 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     8341 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     8401 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     8461 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     8521 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     8581 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     8641 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     8701 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     8761 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     8821 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     8881 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     8941 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     9001 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     9061 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     9121 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     9181 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     9241 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     9301 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     9361 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     9421 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     9481 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     9541 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     9601 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     9661 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     9721 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     9781 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     9841 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     9901 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     9961 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    10021 aagtgcca
//
