LOCUS       sequence-211540-        8597 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  knock in bait for Axolotl Pax7 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8597)
  AUTHORS   Fei JF, Schuez M, Knapp D, Taniguchi Y, Drechsel DN, Tanaka EM
  TITLE     Efficient gene knockin in axolotl and its use to test the role of 
            satellite cells in limb regeneration.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Nov 21;114(47):12501-12506. doi: 
            10.1073/pnas.1706855114. Epub 2017 Oct 31.
  PUBMED    29087939
REFERENCE   2  (bases 1 to 8597)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1706855114"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2017-11-21- 21"; volume: "114"; issue: "47"; pages: 
            "12501-12506"
COMMENT     Sequence Label: pGEMT-Pax7bait-P2a-GAP43-Cherry-T2a-mERt-Cre-Ert
FEATURES             Location/Qualifiers
     source          1..8597
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     22..44
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     36..53
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     37..53
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1461..1517
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1578..2285
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1723..1741)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1991..2010
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             2286..2339
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3288..4310
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="NLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLLS
                     VCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLPR
                     PSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFLG
                     IAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVERW
                     ISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQRY
                     LAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLEDG
                     D"
     CDS             4323..5258
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             4371..5264
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     primer_bind     complement(5348..5367)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5413..5468
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5467..5486)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     promoter        complement(5798..5816)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5799..5816)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5834..5850)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5834..5850)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5847..5869)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5858..5874
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5882..5888,5889..5906,5907..5912))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5927..5948
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6065..6082)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6236..6824)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6316..6335)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6995..7786,7787..7855))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7618..7637
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7856..7960)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8038..8493)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8175..8196)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8387..8406
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattgt
       61 aatacgactc actatagacg cgtatggctt gtctccccgg agctgtcccc agaatgatgc
      121 ggccaggacc agggcagaac tacccacgga ccggtttccc actagaagtg tccactcctc
      181 tgggccaggg cagagtcaac caactggggg gagtgttcat taacgggcgc ccgttgccca
      241 accacatccg ccacaagatc gtggagatgg cacaccacgg cattcggccc tgcgtgattt
      301 cacggcagct acgtgtgtcc cacggctgcg tgtccaagat cctgtgccgg taccaggaga
      361 cggggtccat ccggccagga gccatcggag gcagcaagcc caggcaggtg gccactcctg
      421 acgtggagaa gaagattgag gaatataaga gagagaaccc cgggatgttc agctgggaga
      481 tcagggacag gctcctaaag gatggacact gcgacaggag cacggtgccc tcaggtaacc
      541 ggctggacga aggttcagat gttgagtcag agccagactt gcccttgaaa cgcaagcagc
      601 gacgcagccg caccaccttc actgctgagc agctggagga gttggagaag gcctttgaga
      661 gaacgcacta cccagatatc tacacccgtg aagagttggc gcagagaacc aagctcactg
      721 aggcgagagt acaggtgtgg ttcagcaatc gtagagcaag gtggcgtaaa caggcaggag
      781 ccaatcaact ggcagctttc aatcatctcc tgcccggcgg atttccaccc acaggaatgc
      841 caagtttgcc accctatcag cttccagact ccggctatgc aagtgcccta gcacaagacg
      901 ggggaagcac agtgcacaga cctcagcccc tgccaccctc ctccatgcac cagggtggat
      961 tgtcagctgc tgatgccagt tcagcctacg gaccccgaca cagcttcacc agctattcgg
     1021 acagcttcat gaacccaggc gggccaacta accacatgaa tccggtcagc aatgggctgt
     1081 ctccacaggt gatgagcatc ctcagcaacc ctagtggagt gcagtcccag tcccaggatt
     1141 tctccatctc ccctctgcat ggtggcttgg acacttcgaa ctccatctcg gcaagctgca
     1201 gccagcggag cgactccatc aagtcagtcg atagcctcgc cacctcgcag tcctactgtc
     1261 ctccgaccta cagcaccacc ggctacagcg tggatcctgt ggccagctac caatatgggc
     1321 aatacgggca aactgctgta gactacttgg ctaagaacgt gagcttgtcg acacagagga
     1381 ggatgaagct cggggaccac tcagccgtgt tgggtttgct tcctgtggag acgggacaag
     1441 catacgcatg cggaagcgga gctactaact tcagcctgct gaagcaggct ggagacgtgg
     1501 aggagaaccc tggacctatg ctgtgctgta tgagaagaac caaacaggtt gaaaagaatg
     1561 atgaggacca aaagatcatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg
     1621 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg
     1681 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
     1741 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca
     1801 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg
     1861 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg
     1921 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc
     1981 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga
     2041 tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg
     2101 gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
     2161 ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca
     2221 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt
     2281 acaaggaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat cccggcccta
     2341 tggccggcga tatgagagcc gctaacctgt ggccctcccc actgatgatc aagagaagca
     2401 agaagaactc cctggccctg agcctgaccg ctgatcagat ggtgtccgcc ctgctggacg
     2461 ccgagccccc tatcctgtac agcgagtacg accccaccag gcccttcagc gaggccagca
     2521 tgatgggact gctgacaaac ctggccgatc gcgaactggt gcatatgatc aattgggcca
     2581 agcgcgtgcc cggattcgtg gacctgacac tgcacgacca ggtgcacctg ctggaatgcg
     2641 cttggctgga aattctgatg atcggcctcg tgtggcggag catggaacat cctgtgaagc
     2701 tgctgttcgc ccccaatctg ctgctggacc gcaaccaggg caaatgcgtg gaagggatgg
     2761 tcgaaatctt tgatatgctg ctcgccacca gcagcaggtt cagaatgatg aacctccagg
     2821 gcgaagagtt cgtctgcctg aagtccatca tcctgctgaa cagcggcgtg tacaccttcc
     2881 tgtcctctac cctgaagtcc ctcgaggaaa aggatcacat ccacagggtg ctggataaga
     2941 tcaccgatac cctgatccat ctcatggcta aggccggact cacactccag cagcagcatc
     3001 agagactcgc tcagctgctg ctgatcctga gccacatccg ccacatgtcc aacaagggaa
     3061 tggaacacct ctactctatg aagtgtaaaa acgtcgtccc cctgtacgat ctgctgctcg
     3121 aggccgccga tgcccacaga ctgcacgccc ctacaagcag aggcggcgct agcgtggaag
     3181 agacagacca gtcccacctg gccaccgccg gcagcacaag cagccacagc ctgcagaagt
     3241 actatatcac cggcgaggcc gagggcttcc ccgctacagc tgtcgacaat ttactgaccg
     3301 tacaccaaaa tttgcctgca ttaccggtcg atgcaacgag tgatgaggtt cgcaagaacc
     3361 tgatggacat gttcagggat cgccaggcgt tttctgagca tacctggaaa atgcttctgt
     3421 ccgtttgccg gtcgtgggcg gcatggtgca agttgaataa ccggaaatgg tttcccgcag
     3481 aacctgaaga tgttcgcgat tatcttctat atcttcaggc gcgcggtctg gcagtaaaaa
     3541 ctatccagca acatttgggc cagctaaaca tgcttcatcg tcggtccggg ctgccacgac
     3601 caagtgacag caatgctgtt tcactggtta tgcggcggat ccgaaaagaa aacgttgatg
     3661 ccggtgaacg tgcaaaacag gctctagcgt tcgaacgcac tgatttcgac caggttcgtt
     3721 cactcatgga aaatagcgat cgctgccagg atatacgtaa tctggcattt ctggggattg
     3781 cttataacac cctgttacgt atagccgaaa ttgccaggat cagggttaaa gatatctcac
     3841 gtactgacgg tgggagaatg ttaatccata ttggcagaac gaaaacgctg gttagcaccg
     3901 caggtgtaga gaaggcactt agcctggggg taactaaact ggtcgagcga tggatttccg
     3961 tctctggtgt agctgatgat ccgaataact acctgttttg ccgggtcaga aaaaatggtg
     4021 ttgccgcgcc atctgccacc agccagctat caactcgcgc cctggaaggg atttttgaag
     4081 caactcatcg attgatttac ggcgctaagg atgactccgg tcagagatac ctggcctggt
     4141 ctggacacag tgcccgtgtc ggagccgcgc gagatatggc ccgcgctgga gtttcaatac
     4201 cggagatcat gcaagctggt ggctggacca atgtaaatat tgtcatgaac tatatccgta
     4261 acctggatag tgaaacaggg gcaatggtgc gcctgctgga agatggcgat ctcgagccat
     4321 ctgctggaga catgagagct gccaaccttt ggccaagccc gctcatgatc aaacgctcta
     4381 agaagaacag cctggccttg tccctgacgg ccgaccagat ggtcagtgcc ttgttggatg
     4441 ctgagccccc catactctat tccgagtatg atcctaccag acccttcagt gaagcttcga
     4501 tgatgggctt actgaccaac ctggcagaca gggagctggt tcacatgatc aactgggcga
     4561 agagggtgcc aggctttgtg gatttgaccc tccatgatca ggtccacctt ctagaatgtg
     4621 cctggctaga gatcctgatg attggtctcg tctggcgctc catggagcac ccagtgaagc
     4681 tactgtttgc tcctaacttg ctcttggaca ggaaccaggg aaaatgtgta gagggcatgg
     4741 tggagatctt cgacatgctg ctggctacat catctcggtt ccgcatgatg aatctgcagg
     4801 gagaggagtt tgtgtgcctc aaatctatta ttttgcttaa ttctggagtg tacacatttc
     4861 tgtccagcac cctgaagtct ctggaagaga aggaccatat ccaccgagtc ctggacaaga
     4921 tcacagacac tttgatccac ctgatggcca aggcaggcct gaccctgcag cagcagcacc
     4981 agcggctggc ccagctcctc ctcatcctct cccacatcag gcacatgagt aacaaaggca
     5041 tggagcatct gtacagcatg aagtgcaaga acgtggtgcc cctctatgac ctgctgctgg
     5101 aggcggcgga cgcccaccgc ctacatgcgc ccactagccg tggaggggca tccgtggagg
     5161 agacggacca aagccacttg gccactgcgg gctctacttc atcgcattcc ttgcaaaagt
     5221 attacatcac gggggaggca gagggtttcc ctgccacagc ttaagatatc actcctcagg
     5281 tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa tgccctggct cacaaatacc
     5341 actgagatct ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc
     5401 tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt
     5461 gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagcatt
     5521 tggtttagag tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat
     5581 aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag
     5641 ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat
     5701 ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc
     5761 cagtcatagc tgtccctctt ctcttatgaa gatctattct atagtgtcac ctaaatagct
     5821 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     5881 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     5941 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     6001 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     6061 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     6121 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     6181 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     6241 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     6301 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     6361 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     6421 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     6481 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     6541 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     6601 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     6661 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     6721 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     6781 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     6841 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     6901 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     6961 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     7021 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     7081 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     7141 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     7201 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     7261 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     7321 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     7381 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     7441 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     7501 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     7561 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     7621 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     7681 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     7741 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     7801 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     7861 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     7921 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     7981 cacctgatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggaaa
     8041 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     8101 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     8161 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     8221 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     8281 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     8341 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     8401 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     8461 ccgccgcgct taatgcgccg ctacagggcg cgtccattcg ccattcaggc tgcgcaactg
     8521 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
     8581 tgctgcaagg cgattaa
//
