LOCUS       sequence-211544-        9941 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A zebrafish insulin promoter driving the Brainbow (BB1.0L) cassette.
            Contains red eye marker, and is flanked with I-SceI sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9941)
  AUTHORS   Singh SP, Janjuha S, Hartmann T, Kayisoglu O, Konantz J, Birke S, 
            Murawala P, Alfar EA, Murata K, Eugster A, Tsuji N, Morrissey ER, 
            Brand M, Ninov N
  TITLE     Different developmental histories of beta-cells generate functional 
            and proliferative heterogeneity during islet growth.
  JOURNAL   Nat Commun. 2017 Sep 22;8(1):664. doi: 10.1038/s41467-017-00461-3.
  PUBMED    28939870
REFERENCE   2  (bases 1 to 9941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-09-22"; volume: "8(1):664. doi"; pages: " 
            10.1038/s41467-017-00461-3"
COMMENT     Sequence Label: ins:BB1.0L; cryaa:RFP
FEATURES             Location/Qualifiers
     source          1..9941
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    37..61
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          1256..1828
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     protein_bind    complement(1889..1922)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(1931..1964)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      1977..1986
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1983..2684
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     polyA_signal    2814..2935
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(3018..3051)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      3065..3074
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3071..3073,3074..3076,3077..3790)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    3913..4034
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(4041..4074)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      4082..4091
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4088..4090,4091..4093,4094..4807)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    4930..5051
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    5391..5512
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    5555..5762
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     5751..5768
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(5786..6496)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7080..7100)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7080..7098)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7131..7148)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7131..7147)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7140..7162)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(7288..7743)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7425..7446)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7637..7656
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7770..7874
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7875..7943,7944..8735)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8093..8112)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8906..9494
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9395..9414
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9648..9665
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9782..9803
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9818..9823,9824..9841,9842..9848)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9856..9872
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9861..9883
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9880..9896
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9880..9896
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 taatacgact cactataggg cgaattggag ctcatcacaa gtttgtacaa aaaagcaggc
       61 tccgcggccg cccccttcac cacgcgtaag ctttgtttgt accataataa gctcattata
      121 taatatttaa aatgatactg catcattaat tgatatgttt tctagttatt tttaaatatt
      181 agcaactaaa ttaggaaatt atccatagta aatttaatgt aatattattt gttatattta
      241 acttcagccc acagtctagt ttagttgttg gtcccttatg agaaaaaatt tgggcacctc
      301 tgctctagtg cttgtaaaca tttttgagta tctatagttg aacatgaaag catttttttt
      361 tttttttttt taagaaaatg aagaaagcta aaaacctgta tccactgact tccattaaaa
      421 aaagcaaata ctagggatgt ctatggtgtc ttcttttgtg ttcaacaaat aagcagaaag
      481 taactgctga cagaacatta cattttgtgt gaatcccctt atattgtgtt aaaaactgta
      541 gagaaatcat ctaataataa gaaattatca agaaataaac agaaaatgat ctaatagtat
      601 actataaaat ctttcaaaat ccaggccact accatatgac taaaaccaca catattatgc
      661 tttaaaaaaa gtcacaatga agacgtcaca aacgttcctg aagactaatg actcgtctaa
      721 ccccatctta ccttctgctg tttcactcaa agaaaagcca ccagctgtct ctgtgatctg
      781 ctcattctgt gttaacttgt gttctcaaca attaacaatt aaccaaaagc ctctggagct
      841 gcgtgagcat cacgatgtgt ttgtcttctg tgagcgtggc agtgtccacg aagtcaatgt
      901 gaattggttg gccaaacaca gctaatgtgg catgtgtgat tggacagcca cttaaaagtg
      961 ttaaagtgtg tttacgcaca ggattggttt tccgctgaat caggcaaaac cagcagaacc
     1021 gatctaaact cgagtataaa agtgagtagg agatgctatt cgtcccaaaa catctccacc
     1081 accatctcca ccattcctcg cctctgcttc gagaacaggt gagtgtcgag cgggatggta
     1141 aatctacaga gaatgcggag tgtagcttgt gtacatgttt ttgattaaca gagattgtat
     1201 gtgtgtgttt gtgtcagtgt gaaagggtgg gcgcgccgat cctgagaact tccgggtgag
     1261 tttggggacc cttgattgtt ctttcttttt cgctattgta aaattcatgt tatatggagg
     1321 gggcaaagtt ttcagggtgt tgtttagaat gggaagatgt cccttgtatc accatggacc
     1381 ctcatgataa ttttgtttct ttcactttct actctgttga caaccattgt ctcctcttat
     1441 tttcttttca ttttctgtaa ctttttcgtt aaactttagc ttgcatttgt aacgaatttt
     1501 taaattcact tttgtttatt tgtcagattg taagtacttt ctctaatcac ttttttttca
     1561 aggcaatcag ggtatattat attgtacttc agcacagttt tagagaacaa ttgttataat
     1621 taaatgataa ggtagaatat ttctgcatat aaattctggc tggcgtggaa atattcttat
     1681 tggtagaaac aactacaccc tggtcatcat cctgcctttc tctttatggt tacaatgata
     1741 tacactgttt gagatgagga taaaatactc tgagtccaaa ccgggcccct ctgctaacca
     1801 tgttcatgcc ttcttctctt tcctacagct cctgggcaac gtgctggttg ttgtgctgtc
     1861 tcatcatttt ggcaaagaat tcgatctcat aacttcgtat aggatacttt atacgaagtt
     1921 ataactcgag ataacttcgt atagcataca ttatacgaag ttataccggc agatctgcca
     1981 ccatggtgag caagggcgag gaggtcatca aagagttcat gcgcttcaag gtgcgcatgg
     2041 agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     2101 agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg
     2161 acatcctgtc cccccagttc atgtacggct ccaaggcgta cgtgaagcac cccgccgaca
     2221 tccccgatta caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     2281 tcgaggacgg cggtctggtg accgtgaccc aggactcctc cctgcaggac ggcacgctga
     2341 tctacaaggt gaagatgcgc ggcaccaact tcccccccga cggccccgta atgcagaaga
     2401 agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg
     2461 gcgagatcca ccaggccctg aagctgaagg acggcggcca ctacctggtg gagttcaaga
     2521 ccatctacat ggccaagaag cccgtgcaac tgcccggcta ctactacgtg gacaccaagc
     2581 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag cgctccgagg
     2641 gccgccacca cctgttcctg tacggcatgg acgagctgta caagtaaagt gtgcggccgc
     2701 gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     2761 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     2821 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     2881 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaggcg
     2941 taaattgtaa gcgttaatgc tccgagggcc gccaccacct gttcctgtag gaattggccg
     3001 cgactctagg atctaggata acttcgtata ggatacttta tacgaagtta ttgatccacc
     3061 ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     3121 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     3181 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     3241 ctggcccacc ctcgtgacca ccctgacctg gggcgtgcag tgcttcgccc gctaccccga
     3301 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     3361 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     3421 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     3481 cctggggcac aagctggagt acaacgccat cagcgacaac gtctatatca ccgccgacaa
     3541 gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcagcgt
     3601 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     3661 cgacaaccac tacctgagca cccagtccaa gctgagcaaa gaccccaacg agaagcgcga
     3721 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     3781 gtacaagtaa agcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt
     3841 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     3901 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     3961 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     4021 atcaatgtat cttaatcgag ataacttcgt atagcataca ttatacgaag ttataccggt
     4081 cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     4141 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     4201 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     4261 gcccaccctc gtgaccacct tcggctacgg cctgcagtgc ttcgcccgct accccgacca
     4321 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     4381 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     4441 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     4501 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     4561 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     4621 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     4681 caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     4741 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     4801 caagtaaagc ggccgcgact ctagatcata atcagccata ccacatttgt agaggtttta
     4861 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     4921 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     4981 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     5041 aatgtatctt aaggcgtaaa ttgtaagcgt taattcgagg tccttcctct gcagaggtct
     5101 tgcttctccc ggtcagctga ctccctcccc aagtccttca aatatctcag aacatgggga
     5161 gaaacgggga ccttgtccct cctaaggaac cccagtgctg catgccatca tcccccccac
     5221 cctcgccccc acccccgcca cttctccctc catgcatgtc gacttaatta aggccgcgac
     5281 tctagatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     5341 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     5401 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     5461 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct taatcgaggg
     5521 ggggcccggt acccaccgcg gtggcggcga tctctcccca gcatgcctgc tattgtcttc
     5581 ccaatcctcc cccttgctgt cctgccccaa cccacccccc agaatagaat gacacctact
     5641 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
     5701 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
     5761 agtcgaggct gatcagcggg ccgctttact tgtacagctc gtccatgccg ccggtggagt
     5821 ggcggccctc ggcgcgttcg tactgttcca cgatggtgta gtcctcgttg tgggaggtga
     5881 tgtccaactt gatgttgacg ttgtaggcgc cgggcagctg cacgggcttc ttggccttgt
     5941 aggtggtctt gacctcagcg tcgtagtggc cgccgtcctt cagcttcagc ctctgcttga
     6001 tctcgccctt cagggcgccg tcctcggggt acatccgctc ggaggaggcc tcccagccca
     6061 tggtcttctt ctgcattacg gggccgtcgg aggggaagtt ggtgccgcgc agcttcacct
     6121 tgtagatgaa ctcgccgtcc tgcagggagg agtcctgggt cacggtcacc acgccgccgt
     6181 cctcgaagtt catcacgcgc tcccacttga agccctcggg gaaggacagc ttcaagtagt
     6241 cggggatgtc ggcggggtgc ttcacgtagg ccttggagcc gtacatgaac tgaggggaca
     6301 ggatgtccca ggcgaagggc agggggccac ccttggtcac cttcagcttg gcggtctggg
     6361 tgccctcgta ggggcggccc tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg
     6421 agccctccat gtgcaccttg aagcgcatga actccttgat gatggccatg ttatcctcct
     6481 cgcccttgct caccatgttg tcagacctgg taactcctta ctgtaactta ttgcacctga
     6541 tgaacaaaag atgtgaactg aacgcaagta ggaggctaat agaggctagt acagtacaaa
     6601 gatccccaaa tgatgtgtgc cggccttagt ggaggcagac ccctggttat atacctgtgc
     6661 tgcagaaaga ggggcttaca aatggatctc tctggaatgg cattatctca tggtgggaac
     6721 agtgttgtca gcagaatcag cggggtggac tctgacctga gaatgacagt ctagcactga
     6781 atggctcaga caatactctt gtggccaact atcaccggaa aataagtgcc tgccaaaaca
     6841 aaccagacca gatgaagcta catggctgta gaacgtcagt atttaatgtg ttgtggccac
     6901 ttggttcacc agatttaaga gctaattttt ttaaatatca cagataatca atcgcttttt
     6961 ttttttcttg actgaaaagc ttagactgga aaattctttg aaaaagtttg cactacagaa
     7021 aataaataca taacatcctg cactgaatgc acactattaa tggtacccag cttttgttcc
     7081 ctttagtgag ggttaattgc gcgctaggga taacagggta atacgcgctc actggccgtc
     7141 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     7201 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     7261 cagttgcgca gcctgaatgg cgaatggaaa ttgtaagcgt taatattttg ttaaaattcg
     7321 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     7381 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     7441 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     7501 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     7561 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     7621 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     7681 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     7741 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     7801 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     7861 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     7921 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     7981 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     8041 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     8101 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     8161 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     8221 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     8281 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     8341 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     8401 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     8461 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     8521 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     8581 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     8641 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     8701 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     8761 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     8821 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     8881 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     8941 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     9001 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     9061 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     9121 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     9181 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     9241 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     9301 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     9361 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     9421 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     9481 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     9541 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     9601 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     9661 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     9721 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     9781 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     9841 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     9901 attacgccaa gcgcgttagg gataacaggg taatgcgcgc g
//
