LOCUS       sequence-211557-        8968 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  miR-E (miR-30 variant)-based RNAi .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8968)
  AUTHORS   Fellmann C, Hoffmann T, Sridhar V, Hopfgartner B, Muhar M, Roth M, 
            Lai DY, Barbosa IA, Kwon JS, Guan Y, Sinha N, Zuber J
  TITLE     An optimized microRNA backbone for effective single-copy RNAi.
  JOURNAL   Cell Rep. 2013 Dec 26;5(6):1704-13. doi: 
            10.1016/j.celrep.2013.11.020. Epub 2013 Dec 12.
  PUBMED    24332856
REFERENCE   2  (bases 1 to 8968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2013.11.020"; journalName: "Cell Rep"; date: 
            "2013-12-26- 26"; volume: "5"; issue: "6"; pages: "1704-13"
COMMENT     Sequence Label: SGEP
FEATURES             Location/Qualifiers
     source          1..8968
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    304..421
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        459..865
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             join(888..890,891..893,894..1607)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(933..954)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1194..1213)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1541..1562
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     ncRNA           1801..1815
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        1994..2493
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2029..2052)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2426..2445
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2514..3113
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2514..2533)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3010..3030
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3194..3782
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3247..3267)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3665..3676)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3989..4222
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4294..4415
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4331..4350)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4385..4404
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4455..4590
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4610..4629)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4611..4629)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4639..4656)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4639..4655)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4648..4670)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4797..5252
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4884..4903)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5094..5115
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5278..5382
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5383..5451,5452..6243)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5601..5620)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6414..7002
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6903..6922
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7156..7173
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7290..7311
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7326..7331,7332..7349,7350..7356)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7364..7380
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7369..7391
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7388..7404
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7388..7404
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7423..7443
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7425..7443
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7471..7697
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7698..7878
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7925..8050
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8543..8776
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             join(8961..8968,1..37)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
       61 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
      121 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
      181 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
      241 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
      301 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
      361 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
      421 tatcgatcac gagactagcc tcgatcgagc tagctgcagt aacgccattt tgcaaggcat
      481 ggaaaaatac caaaccaaga atagagaagt tcagatcaag ggcgggtaca tgaaaatagc
      541 taacgttggg ccaaacagga tatctgcggt gagcagtttc ggccccggcc cggggccaag
      601 aacagatggt caccgcagtt tcggccccgg cccgaggcca agaacagatg gtccccagat
      661 atggcccaac cctcagcagt ttcttaagac ccatcagatg tttccaggct cccccaagga
      721 cctgaaatga ccctgcgcct tatttgaatt aaccaatcag cctgcttctc gcttctgttc
      781 gcgcgcttct gcttcccgag ctctataaaa gagctcacaa cccctcactc ggcgcgccag
      841 tcctccgaca gactgagtcg gccggtggat ctaccggtgg atccaccatg gtgagcaagg
      901 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
      961 gccacaagtt cagcgtgtct ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     1021 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     1081 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     1141 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     1201 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     1261 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     1321 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggcga
     1381 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     1441 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     1501 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     1561 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaatga cgtacggcgg
     1621 ccgcctcgac tagggataac agggtaattg tttgaatgag gcttcagtac tttacagaat
     1681 cgttgcctgc acatcttgga aacacttgct gggattactt cgacttctta acccaacaga
     1741 aggctcgaga aggtatattg ctgttgacag tgagcgcagg aattataatg cttatctata
     1801 gtgaagccac agatgtatag ataagcatta taattcctat gcctactgcc tcggacttca
     1861 aggggctaga attcgagcaa ttatcttgtt tactaaaact gaataccttg ctatctcttt
     1921 gatacatttt tacaaagctg aattaaaatg gtataaatta aatcactttt ttcaattgac
     1981 gcgtaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta
     2041 gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca
     2101 tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact
     2161 cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa
     2221 atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag
     2281 cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag
     2341 aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc
     2401 gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg
     2461 ctgttctcct cttcctcatc tccgggcctt tcgacctgca gcccaagctt accatgaccg
     2521 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
     2581 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca
     2641 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
     2701 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
     2761 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
     2821 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
     2881 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
     2941 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
     3001 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
     3061 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc
     3121 cccacgaccc gcagcgcccg accgaaagga gcgcacgacc ccatgcatgt cgacttcgaa
     3181 acgcgtctgg aacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     3241 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     3301 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     3361 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     3421 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     3481 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     3541 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
     3601 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     3661 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     3721 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     3781 gcctggaatt aattctgcag tcgagaccta gaaaaacatg gagcaatcac aagtagcaat
     3841 acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt
     3901 tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     3961 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     4021 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     4081 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     4141 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     4201 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     4261 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     4321 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     4381 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     4441 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     4501 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     4561 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga
     4621 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     4681 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     4741 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
     4801 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     4861 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     4921 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     4981 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     5041 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     5101 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     5161 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     5221 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
     5281 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     5341 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     5401 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     5461 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     5521 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     5581 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     5641 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     5701 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     5761 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     5821 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     5881 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     5941 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     6001 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     6061 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     6121 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     6181 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     6241 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     6301 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     6361 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     6421 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     6481 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     6541 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     6601 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     6661 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     6721 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     6781 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     6841 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     6901 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     6961 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     7021 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     7081 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     7141 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     7201 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     7261 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     7321 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     7381 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
     7441 agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt
     7501 gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat
     7561 gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc
     7621 tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc
     7681 tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     7741 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     7801 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     7861 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
     7921 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
     7981 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
     8041 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
     8101 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
     8161 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
     8221 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
     8281 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
     8341 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct
     8401 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
     8461 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
     8521 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
     8581 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
     8641 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
     8701 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
     8761 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
     8821 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
     8881 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
     8941 aatcgcaaaa ccagcaagaa aagaatga
//
