LOCUS       sequence-211563-        7860 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus rescue vector, VSVGED pseudotype, hShh.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7860)
  AUTHORS   Nacu E, Gromberg E, Oliveira CR, Drechsel D, Tanaka EM
  TITLE     FGF8 and SHH substitute for anterior-posterior tissue interactions 
            to induce limb regeneration.
  JOURNAL   Nature. 2016 May 19;533(7603):407-10. doi: 10.1038/nature17972.
  PUBMED    27120163
REFERENCE   2  (bases 1 to 7860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2016-05-19- 19"; volume: "533"; issue: "7603"; 
            pages: "407-10"
COMMENT     Sequence Label: pOEM1_pCMV:hShh-pPH:vsvged
FEATURES             Location/Qualifiers
     source          1..7860
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     3'UTR           226..598
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     enhancer        699..1002
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1003..1206
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1157..1177
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      1245..1254
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2723..2743)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3141..3152)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3387..3521
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3424..3443)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3478..3497
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(3634..3666)
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     sig_peptide     complement(3862..3921)
                     /codon_start=1
                     /gene="Autographa californica gp64"
                     /product="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
                     /label=GP64 signal sequence
                     /translation="MVSAIVLYVLLAAAAHSAFA"
     promoter        complement(3984..4075)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     complement(4042..4059)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             4161..4766
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     CDS             complement(4804..4928)
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     primer_bind     complement(5538..5557)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5539..5557)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5568..5585)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5568..5584)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5577..5599)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        5900..6004
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6005..6073,6074..6865)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6223..6242)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7036..7624
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7525..7544
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7717..7734
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 caaatttgat cgcggacgtg ttagccgaca caattaatag gcgtcgtgtg gctatggcaa
       61 aatcgtcttc ggaagcaact tctaacgacg agggttggga cgacgacgat aatcggccta
      121 ataaagctaa cacgcccgat gttaaatatg tccaagcgtt atttaacgtg tttacgtcga
      181 gtcaattgta cactaacgac agtgatgaaa gaaatacaaa agcgcataat attttgaacg
      241 acgtcgaacc tttattacaa aacaaaacac aaacgaatat cgacaaagct agattgctgc
      301 tacaagattt ggcaagtttt gtggcgttga gcgaaaatcc attagatagt ccagccatcg
      361 gttcggaaaa acaacccttg tttgaaacta atcgaaacct attttacaaa tctattgagg
      421 atttaatatt taaattcaga tataaagacg ctgaaaatca tttgattttc gctctaacat
      481 accaccctaa agattataaa tttaatgaat tattaaaata cgtacaacaa ttgtctgtaa
      541 atcaacaacg cacagaatct agcgcttaat aaatgtacta ataacaatgt atcgtgtttt
      601 attaattaag atctgcggat gcaggctccg ccatgcatta gttattaata gtaatcaatt
      661 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
      721 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
      781 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
      841 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
      901 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
      961 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     1021 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     1081 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     1141 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     1201 agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggtcgccacc atgggcgaga
     1261 tgctgctgct ggcgagatgt ctgctgctag tcctcgtctc ctcgctgctg gtatgctcgg
     1321 gactggcgtg cggaccgggc agggggttcg ggaagaggag gcaccccaaa aagctgaccc
     1381 ctttagccta caagcagttt atccccaatg tggccgagaa gaccctaggc gccagcggaa
     1441 ggtatgaagg gaagatctcc agaaactccg agcgatttaa ggaactcacc cccaattaca
     1501 accccgacat catatttaag gatgaagaaa acaccggagc ggacaggctg atgactcaga
     1561 ggtgtaagga caagttgaac gctttggcca tctcggtgat gaaccagtgg ccaggagtga
     1621 aactgcgggt gaccgagggc tgggacgaag atggccacca ctcagaggag tctctgcact
     1681 acgagggccg cgcagtggac atcaccacgt ctgaccgcga ccgcagcaag tacggcatgc
     1741 tggcccgcct ggcggtggag gccggcttcg actgggtgta ctacgagtcc aaggcacata
     1801 tccactgctc ggtgaaagca gagaactcgg tggcggccaa atcgggaggc tgcttcccgg
     1861 gctcggccac ggtgcacctg gagcagggcg gcaccaagct ggtgaaggac ctgagccccg
     1921 gggaccgcgt gctggcggcg gacgaccagg gccggctgct ctacagcgac ttcctcactt
     1981 tcctggaccg cgacgacggc gccaagaagg tcttctacgt gatcgagacg cgggagccgc
     2041 gcgagcgcct gctgctcacc gccgcgcacc tgctctttgt ggcgccgcac aacgactcgg
     2101 ccaccgggga gcccgaggcg tcctcgggct cggggccgcc ttccgggggc gcactggggc
     2161 ctcgggcgct gttcgccagc cgcgtgcgcc cgggccagcg cgtgtacgtg gtggccgagc
     2221 gtgacgggga ccgccggctc ctgcccgccg ctgtgcacag cgtgacccta agcgaggagg
     2281 ccgcgggcgc ctacgcgccg ctcacggccc agggcaccat tctcatcaac cgggtgctgg
     2341 cctcgtgcta cgcggtcatc gaggagcaca gctgggcgca ccgggccttc gcgcccttcc
     2401 gcctggcgca cgcgctcctg gctgcactgg cgcccgcgcg cacggaccgc ggcggggaca
     2461 gcggcggcgg ggaccgcggg ggcggcggcg gcagagtagc cctaaccgct ccaggtgctg
     2521 ccgacgctcc gggtgcgggg gccaccgcgg gcatccactg gtactcgcag ctgctctacc
     2581 aaataggcac ctggctcctg gacagcgagg ccctgcaccc gctgggcatg gcggtcaagt
     2641 ccagctgagc ggccgctaga cgcgtcgaca atcaacctct ggattacaaa atttgtgaaa
     2701 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     2761 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     2821 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     2881 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     2941 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     3001 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     3061 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     3121 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     3181 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     3241 tttgggccgc ctccccgaag cttgtcgaga agtactagag gatcataatc agccatacca
     3301 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     3361 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     3421 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     3481 gtttgtccaa actcatcaat gtatcttatc atgtctggat ctgatcactg gcgcgccgtg
     3541 atgcgtattt ctgaatcttt gtaaaatagc acacaagact ccaacgcgtt tggcgtttta
     3601 ttttcttgct cgactctagt ttattaggcc ttactttcca agtcggttca tctctatgtc
     3661 tgtataaatc tgtcttttct tggtgtgctt taatttaatg caaagatgga taccaactcg
     3721 gagaaccaag aatagtccaa tgattaaccc tatgataaag aaaaaagagg caatagagct
     3781 tttccaacta ctgaaccaac cttctacaag ctcgattgga tttttggata gcccagtatc
     3841 accccggggt acctgcagat ccgcaaaggc agaatgcgcc gccgccgcca aaagcacata
     3901 taaaacaata gcgcttacca tcttgcttgt gtgttcctta ttgaagcctt ggtgtgactg
     3961 atttactagt agcatagatc cgtatttata ggttttttta ttacaaaact gttacgaaaa
     4021 cagtaaaata cttatttatt tgcgagatgg ttatcatttt aattatctcc atgatccagc
     4081 caaccactag aactatagct agagtcctgg gcgaacaaaa tggtggcagc ttgtgatatt
     4141 agtttgtgcg tctcattaca atggctgtta tttttaacaa caaacaactg ctcgcagaca
     4201 atagtataga aaagggaggt gaactgtttt tgtttaacgg ttcgtacaac attttggaaa
     4261 gttatgttaa tccggtgctg ctaaaaaatg gtgtaattga actagaagaa gctgcgtact
     4321 atgccggcaa catattgtac aaaaccgacg atcccaaatt cattgattat ataaatttaa
     4381 taattaaagc aacacactcc gaagaactac cagaaaatag cactgttgta aattacagaa
     4441 aaactatgcg cagcggtact atacacccca ttaaaaaaga catatatatt tatgacaaca
     4501 aaaaatttac tctatacgat agatacatat atggatacga taataactat gttaattttt
     4561 atgaggagaa aaatgaaaaa gagaaggaat acgaagaaga agacgacaag gcgtctagtt
     4621 tatgtgaaaa taaaattata ttgtcgcaaa ttaactgtga atcatttgaa aatgatttta
     4681 aatattacct cagcgattat aactacgcgt tttcaattat agataatact acaaatgttc
     4741 ttgttgcgtt tggtttgtat cgttaataaa aaacaaattt agcatttata attgttttat
     4801 tattcaataa ttacaaatag gattgagacc cttgcagttg ccagcaaacg gacagagctt
     4861 gtccacagtt ttcatttttt ggcagtttgg tggtttatac acatcttggg atttgattaa
     4921 aagatgcatg acttcaccca cacacttgga gtcgtggttg tagaaatgcg tcagagcctt
     4981 gattaaacat cggttttcac acttggcaca cgaaaccacg tttgcaatgt aacagtttag
     5041 tataaacctc ttcctgtaca taccgccgcg cggtacattg ttttctttca aatcgttcaa
     5101 tatttttttt atacacggag gcatattaat cttgttgttc agcatgtcca cgacattttt
     5161 ctttccttct acgcattctt tcatacaaat gtttctcttg ctgcgttttt taaaattgga
     5221 atttttaatt tcattaatag tgggcggagc cgttaacagc atgtacaatc gcagtccgga
     5281 aattttgacc aaaaccccgc cattgtagaa taccgtgtat ggagttaggg tcaatttatc
     5341 aataaaatca tcgggatcaa ttaaatatgt agctttcttg tctaaacatg acgctctaat
     5401 gagcggactc cacacgttat acgatgcatt cgccatactt cgcggcttct cgcaccagcg
     5461 gtcctgattc gactaacggt cgattggatg gcttaatagt tttaacgtca cggccttgac
     5521 ggccttccgc caattcgccc tatagtgagt cgtattacgt cgcgctcact ggccgtcgtt
     5581 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     5641 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgaaacgcc cttcccaaca
     5701 gttgcgcagc ctgaatggcg aatgggagcg ccctgtagcg gccactcaac cctatctcgg
     5761 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     5821 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg
     5881 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     5941 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     6001 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     6061 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     6121 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     6181 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     6241 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     6301 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     6361 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     6421 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     6481 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     6541 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     6601 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     6661 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     6721 ttctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     6781 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     6841 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     6901 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     6961 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     7021 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     7081 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     7141 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     7201 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     7261 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     7321 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     7381 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     7441 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     7501 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     7561 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     7621 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     7681 ttaggcaccc caggctttac ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     7741 gagagcgccc aatacgcaag gaaacagcta tgaccatgtt aatgcagctg gcacgacagg
     7801 tttcccgact ggaaagcggg cagtgagcgg aaggcccatg aggcccaaaa agctcaacta
//
