LOCUS       sequence-211569-        7691 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus rescue vector, VSVGED pseudotype, hShh.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7691)
  AUTHORS   Wagner I, Wang H, Weissert PM, Straube WL, Shevchenko A, Gentzel M, 
            Brito G, Tazaki A, Oliveira C, Sugiura T, Shevchenko A, Simon A, 
            Drechsel DN, Tanaka EM
  TITLE     Serum Proteases Potentiate BMP-Induced Cell Cycle Re-entry of 
            Dedifferentiating Muscle Cells during Newt Limb Regeneration.
  JOURNAL   Dev Cell. 2017 Mar 27;40(6):608-617.e6. doi: 
            10.1016/j.devcel.2017.03.002.
  PUBMED    28350991
REFERENCE   2  (bases 1 to 7691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2017.03"; journalName: "Dev Cell"; date: 
            "2017-03-27- 27"; volume: "40"; issue: "6"; pages: "608-617.e6"
COMMENT     Sequence Label: pOEM1_pCMV:bmp4-pPH:vsvged
FEATURES             Location/Qualifiers
     source          1..7691
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..600
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     501..520
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     693..710
                     /label=L4440
                     /note="L4440 vector, forward primer"
     3'UTR           1062..1434
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     enhancer        1535..1838
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1839..2042
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1993..2013
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(3390..3410)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3808..3819)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4054..4188
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4091..4110)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4145..4164
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(4301..4333)
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     sig_peptide     complement(4529..4588)
                     /codon_start=1
                     /gene="Autographa californica gp64"
                     /product="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
                     /label=GP64 signal sequence
                     /translation="MVSAIVLYVLLAAAAHSAFA"
     promoter        complement(4651..4742)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     complement(4709..4726)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             4828..5433
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     CDS             complement(5471..5595)
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     primer_bind     complement(6205..6224)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6206..6224)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6235..6252)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6235..6251)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6244..6266)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        6567..6671
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6672..6740,6741..7532)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6890..6909)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
       61 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
      121 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
      181 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
      241 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
      301 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
      361 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
      421 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
      481 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
      541 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
      601 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcattag
      661 gcaccccagg ctttacccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaga
      721 gcgcccaata cgcaaggaaa cagctatgac catgttaatg cagctggcac gacaggtttc
      781 ccgactggaa agcgggcagt gagcggaagg cccatgaggc ccaaaaagct caactacaaa
      841 tttgatcgcg gacgtgttag ccgacacaat taataggcgt cgtgtggcta tggcaaaatc
      901 gtcttcggaa gcaacttcta acgacgaggg ttgggacgac gacgataatc ggcctaataa
      961 agctaacacg cccgatgtta aatatgtcca agcgttattt aacgtgttta cgtcgagtca
     1021 attgtacact aacgacagtg atgaaagaaa tacaaaagcg cataatattt tgaacgacgt
     1081 cgaaccttta ttacaaaaca aaacacaaac gaatatcgac aaagctagat tgctgctaca
     1141 agatttggca agttttgtgg cgttgagcga aaatccatta gatagtccag ccatcggttc
     1201 ggaaaaacaa cccttgtttg aaactaatcg aaacctattt tacaaatcta ttgaggattt
     1261 aatatttaaa ttcagatata aagacgctga aaatcatttg attttcgctc taacatacca
     1321 ccctaaagat tataaattta atgaattatt aaaatacgta caacaattgt ctgtaaatca
     1381 acaacgcaca gaatctagcg cttaataaat gtactaataa caatgtatcg tgttttatta
     1441 attaagatct gcggatgcag gctccgccat gcattagtta ttaatagtaa tcaattacgg
     1501 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     1561 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     1621 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     1681 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     1741 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     1801 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     1861 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     1921 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     1981 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
     2041 ctggtttagt gaaccgtcag atccgctagc gctaccggtg ccgccaccat gattcctggt
     2101 aaccgaatgc tgatggtcgt tttattatgc caagtcctgc taggaggcgc gagccatgct
     2161 agtttgatac ctgagacggg gaagaaaaaa gtcgccgaga ttcagggcca cgcgggagga
     2221 cgccgctcag ggcagagcca tgagctcctg cgggacttcg aggcgacact tctgcagatg
     2281 tttgggctgc gccgccgccc gcagcctagc aagagtgccg tcattccgga ctacatgcgg
     2341 gatctttacc ggcttcagtc tggggaggag gaggaagagc agatccacag cactggtctt
     2401 gagtatcctg agcgcccggc cagccgggcc aacaccgtga ggagcttcca ccacgaagaa
     2461 catctggaga acatcccagg gaccagtgaa aactctgctt ttcgtttcct ctttaacctc
     2521 agcagcatcc ctgagaacga ggcgatctcc tctgcagagc ttcggctctt ccgggagcag
     2581 gtggaccagg gccctgattg ggaaaggggc ttccaccgta taaacattta tgaggttatg
     2641 aagcccccag cagaagtggt gcctgggcac ctcatcacac gactactgga cacgagactg
     2701 gtccaccaca atgtgacacg gtgggaaact tttgatgtga gccctgcggt ccttcgctgg
     2761 acccgggaga agcagccaaa ctatgggcta gccattgagg tgactcacct ccatcagact
     2821 cggacccacc agggccagca tgtcaggatt agccgatcgt tacctcaagg gagtgggaat
     2881 tgggcccagc tccggcccct cctggtcacc tttggccatg atggccgggg ccatgccttg
     2941 acccgacgcc ggagggccaa gcgtagccct aagcatcact cacagcgggc caggaagaag
     3001 aataagaact gccggcgcca ctcgctctat gtggacttca gcgatgtggg ctggaatgac
     3061 tggattgtgg ccccaccagg ctaccaggcc ttctactgcc atggggactg cccctttcca
     3121 ctggctgacc acctcaactc aaccaaccat gccattgtgc agaccctggt caattctgtc
     3181 aattccagta tccccaaagc ctgttgtgtg cccactgaac tgagtgccat ctccatgctg
     3241 tacctggatg agtatgataa ggtggtactg aaaaattatc aggagatggt agtagaggga
     3301 tgtgggtgcc gctgagcggc cgctagacgc gtcgacaatc aacctctgga ttacaaaatt
     3361 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     3421 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     3481 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     3541 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     3601 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     3661 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     3721 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
     3781 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     3841 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     3901 atctcccttt gggccgcctc cccgaagctt gtcgagaagt actagaggat cataatcagc
     3961 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     4021 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     4081 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     4141 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatctg atcactggcg
     4201 cgccgtgatg cgtatttctg aatctttgta aaatagcaca caagactcca acgcgtttgg
     4261 cgttttattt tcttgctcga ctctagttta ttaggcctta ctttccaagt cggttcatct
     4321 ctatgtctgt ataaatctgt cttttcttgg tgtgctttaa tttaatgcaa agatggatac
     4381 caactcggag aaccaagaat agtccaatga ttaaccctat gataaagaaa aaagaggcaa
     4441 tagagctttt ccaactactg aaccaacctt ctacaagctc gattggattt ttggatagcc
     4501 cagtatcacc ccggggtacc tgcagatccg caaaggcaga atgcgccgcc gccgccaaaa
     4561 gcacatataa aacaatagcg cttaccatct tgcttgtgtg ttccttattg aagccttggt
     4621 gtgactgatt tactagtagc atagatccgt atttataggt ttttttatta caaaactgtt
     4681 acgaaaacag taaaatactt atttatttgc gagatggtta tcattttaat tatctccatg
     4741 atccagccaa ccactagaac tatagctaga gtcctgggcg aacaaaatgg tggcagcttg
     4801 tgatattagt ttgtgcgtct cattacaatg gctgttattt ttaacaacaa acaactgctc
     4861 gcagacaata gtatagaaaa gggaggtgaa ctgtttttgt ttaacggttc gtacaacatt
     4921 ttggaaagtt atgttaatcc ggtgctgcta aaaaatggtg taattgaact agaagaagct
     4981 gcgtactatg ccggcaacat attgtacaaa accgacgatc ccaaattcat tgattatata
     5041 aatttaataa ttaaagcaac acactccgaa gaactaccag aaaatagcac tgttgtaaat
     5101 tacagaaaaa ctatgcgcag cggtactata caccccatta aaaaagacat atatatttat
     5161 gacaacaaaa aatttactct atacgataga tacatatatg gatacgataa taactatgtt
     5221 aatttttatg aggagaaaaa tgaaaaagag aaggaatacg aagaagaaga cgacaaggcg
     5281 tctagtttat gtgaaaataa aattatattg tcgcaaatta actgtgaatc atttgaaaat
     5341 gattttaaat attacctcag cgattataac tacgcgtttt caattataga taatactaca
     5401 aatgttcttg ttgcgtttgg tttgtatcgt taataaaaaa caaatttagc atttataatt
     5461 gttttattat tcaataatta caaataggat tgagaccctt gcagttgcca gcaaacggac
     5521 agagcttgtc cacagttttc attttttggc agtttggtgg tttatacaca tcttgggatt
     5581 tgattaaaag atgcatgact tcacccacac acttggagtc gtggttgtag aaatgcgtca
     5641 gagccttgat taaacatcgg ttttcacact tggcacacga aaccacgttt gcaatgtaac
     5701 agtttagtat aaacctcttc ctgtacatac cgccgcgcgg tacattgttt tctttcaaat
     5761 cgttcaatat tttttttata cacggaggca tattaatctt gttgttcagc atgtccacga
     5821 catttttctt tccttctacg cattctttca tacaaatgtt tctcttgctg cgttttttaa
     5881 aattggaatt tttaatttca ttaatagtgg gcggagccgt taacagcatg tacaatcgca
     5941 gtccggaaat tttgaccaaa accccgccat tgtagaatac cgtgtatgga gttagggtca
     6001 atttatcaat aaaatcatcg ggatcaatta aatatgtagc tttcttgtct aaacatgacg
     6061 ctctaatgag cggactccac acgttatacg atgcattcgc catacttcgc ggcttctcgc
     6121 accagcggtc ctgattcgac taacggtcga ttggatggct taatagtttt aacgtcacgg
     6181 ccttgacggc cttccgccaa ttcgccctat agtgagtcgt attacgtcgc gctcactggc
     6241 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     6301 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg aaacgccctt
     6361 cccaacagtt gcgcagcctg aatggcgaat gggagcgccc tgtagcggcc actcaaccct
     6421 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     6481 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     6541 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     6601 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     6661 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     6721 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     6781 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     6841 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     6901 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     6961 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     7021 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     7081 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     7141 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     7201 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     7261 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     7321 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     7381 agcgtggttc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     7441 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     7501 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     7561 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     7621 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     7681 tagaaaagat c
//
