LOCUS       sequence-211572-        7653 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian vector for expressing effector domains  fused to dCas9..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7653)
  AUTHORS   Goubert D, Koncz M, Kiss A, Rots MG
  TITLE     Establishment of Cell Lines Stably Expressing dCas9-Fusions to 
            Address Kinetics of Epigenetic Editing.
  JOURNAL   Methods Mol Biol. 2018;1767:395-415. doi: 
            10.1007/978-1-4939-7774-1_22.
  PUBMED    29524148
REFERENCE   2  (bases 1 to 7653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2018"; volume: "1767:395-415. doi"; pages: " 
            10.1007/978-1-4939-7774-1_22"
COMMENT     Sequence Label: pdCas9-NED
FEATURES             Location/Qualifiers
     source          1..7653
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(36..827,828..896))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     659..678
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(897..1001)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1080..1099)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1271..1650
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1651..1854
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1805..1825
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1896..1915
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1896..1914
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1922..1931
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1928..5329,5330..5332,5333..5929,5930,5931..5933,
                     5934..5935,5936..5938,5939..6031)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(1928..1954,1955..1957,1958..4444,4445..4447,
                     4448..6031)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6038..6058
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6065..6130
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6187..6204
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6227..6244)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6233..6457
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6528..6544)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6528..6544)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6541..6563)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6552..6568
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6576..6582,6583..6600,6601..6606))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6621..6642
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6759..6776)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6930..7518)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7010..7029)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
       61 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
      121 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
      181 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
      241 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
      301 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
      361 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
      421 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
      481 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
      541 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
      601 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
      661 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
      721 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
      781 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
      841 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
      901 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
      961 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     1021 ccacctgacg tcgacggatc gggagatcga tctcccgatc ccctagggtc gactctcagt
     1081 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
     1141 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
     1201 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga
     1261 tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     1321 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     1381 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     1441 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     1501 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     1561 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1621 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     1681 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     1741 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     1801 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta
     1861 gtgaaccgtc agatccgcta gagatccgcg gccgctaata cgactcacta tagggagagc
     1921 cgccaccatg gataaaaagt attctattgg tttagccatc ggcactaatt ccgttggatg
     1981 ggctgtcata accgatgaat acaaagtacc ttcaaagaaa tttaaggtgt tggggaacac
     2041 agaccgtcat tcgattaaaa agaatcttat cggtgccctc ctattcgata gtggcgaaac
     2101 ggcagaggcg actcgcctga aacgaaccgc tcggagaagg tatacacgtc gcaagaaccg
     2161 aatatgttac ttacaagaaa tttttagcaa tgagatggcc aaagttgacg attctttctt
     2221 tcaccgtttg gaagagtcct tccttgtcga agaggacaag aaacatgaac ggcaccccat
     2281 ctttggaaac atagtagatg aggtggcata tcatgaaaag tacccaacga tttatcacct
     2341 cagaaaaaag ctagttgact caactgataa agcggacctg aggttaatct acttggctct
     2401 tgcccatatg ataaagttcc gtgggcactt tctcattgag ggtgatctaa atccggacaa
     2461 ctcggatgtc gacaaactgt tcatccagtt agtacaaacc tataatcagt tgtttgaaga
     2521 gaaccctata aatgcaagtg gcgtggatgc gaaggctatt cttagcgccc gcctctctaa
     2581 atcccgacgg ctagaaaacc tgatcgcaca attacccgga gagaagaaaa atgggttgtt
     2641 cggtaacctt atagcgctct cactaggcct gacaccaaat tttaagtcga acttcgactt
     2701 agctgaagat gccaaattgc agcttagtaa ggacacgtac gatgacgatc tcgacaatct
     2761 actggcacaa attggagatc agtatgcgga cttatttttg gctgccaaaa accttagcga
     2821 tgcaatcctc ctatctgaca tactgagagt taatactgag attaccaagg cgccgttatc
     2881 cgcttcaatg atcaaaaggt acgatgaaca tcaccaagac ttgacacttc tcaaggccct
     2941 agtccgtcag caactgcctg agaaatataa ggaaatattc tttgatcagt cgaaaaacgg
     3001 gtacgcaggt tatattgacg gcggagcgag tcaagaggaa ttctacaagt ttatcaaacc
     3061 catattagag aagatggatg ggacggaaga gttgcttgta aaactcaatc gcgaagatct
     3121 actgcgaaag cagcggactt tcgacaacgg tagcattcca catcaaatcc acttaggcga
     3181 attgcatgct atacttagaa ggcaggagga tttttatccg ttcctcaaag acaatcgtga
     3241 aaagattgag aaaatcctaa cctttcgcat accttactat gtgggacccc tggcccgagg
     3301 gaactctcgg ttcgcatgga tgacaagaaa gtccgaagaa acgattactc catggaattt
     3361 tgaggaagtt gtcgataaag gtgcgtcagc tcaatcgttc atcgagagga tgaccaactt
     3421 tgacaagaat ttaccgaacg aaaaagtatt gcctaagcac agtttacttt acgagtattt
     3481 cacagtgtac aatgaactca cgaaagttaa gtatgtcact gagggcatgc gtaaacccgc
     3541 ctttctaagc ggagaacaga agaaagcaat agtagatctg ttattcaaga ccaaccgcaa
     3601 agtgacagtt aagcaattga aagaggacta ctttaagaaa attgaatgct tcgattctgt
     3661 cgagatctcc ggggtagaag atcgatttaa tgcgtcactt ggtacgtatc atgacctcct
     3721 aaagataatt aaagataagg acttcctgga taacgaagag aatgaagata tcttagaaga
     3781 tatagtgttg actcttaccc tctttgaaga tcgggaaatg attgaggaaa gactaaaaac
     3841 atacgctcac ctgttcgacg ataaggttat gaaacagtta aagaggcgtc gctatacggg
     3901 ctggggacga ttgtcgcgga aacttatcaa cgggataaga gacaagcaaa gtggtaaaac
     3961 tattctcgat tttctaaaga gcgacggctt cgccaatagg aactttatgc agctgatcca
     4021 tgatgactct ttaaccttca aagaggatat acaaaaggca caggtttccg gacaagggga
     4081 ctcattgcac gaacatattg cgaatcttgc tggttcgcca gccatcaaaa agggcatact
     4141 ccagacagtc aaagtagtgg atgagctagt taaggtcatg ggacgtcaca aaccggaaaa
     4201 cattgtaatc gagatggcac gcgaaaatca aacgactcag aaggggcaaa aaaacagtcg
     4261 agagcggatg aagagaatag aagagggtat taaagaactg ggcagccaga tcttaaagga
     4321 gcatcctgtg gaaaataccc aattgcagaa cgagaaactt tacctctatt acctacaaaa
     4381 tggaagggac atgtatgttg atcaggaact ggacataaac cgtttatctg attacgacgt
     4441 cgatgccatt gtaccccaat cctttttgaa ggacgattca atcgacaata aagtgcttac
     4501 acgctcggat aagaaccgag ggaaaagtga caatgttcca agcgaggaag tcgtaaagaa
     4561 aatgaagaac tattggcggc agctcctaaa tgcgaaactg ataacgcaaa gaaagttcga
     4621 taacttaact aaagctgaga ggggtggctt gtctgaactt gacaaggccg gatttattaa
     4681 acgtcagctc gtggaaaccc gccaaatcac aaagcatgtt gcacagatac tagattcccg
     4741 aatgaatacg aaatacgacg agaacgataa gctgattcgg gaagtcaaag taatcacttt
     4801 aaagtcaaaa ttggtgtcgg acttcagaaa ggattttcaa ttctataaag ttagggagat
     4861 aaataactac caccatgcgc acgacgctta tcttaatgcc gtcgtaggga ccgcactcat
     4921 taagaaatac ccgaagctag aaagtgagtt tgtgtatggt gattacaaag tttatgacgt
     4981 ccgtaagatg atcgcgaaaa gcgaacagga gataggcaag gctacagcca aatacttctt
     5041 ttattctaac attatgaatt tctttaagac ggaaatcact ctggcaaacg gagagatacg
     5101 caaacgacct ttaattgaaa ccaatgggga gacaggtgaa atcgtatggg ataagggccg
     5161 ggacttcgcg acggtgagaa aagttttgtc catgccccaa gtcaacatag taaagaaaac
     5221 tgaggtgcag accggagggt tttcaaagga atcgattctt ccaaaaagga atagtgataa
     5281 gctcatcgct cgtaaaaagg actgggaccc gaaaaagtac ggtggcttcg atagccctac
     5341 agttgcctat tctgtcctag tagtggcaaa agttgagaag ggaaaatcca agaaactgaa
     5401 gtcagtcaaa gaattattgg ggataacgat tatggagcgc tcgtcttttg aaaagaaccc
     5461 catcgacttc cttgaggcga aaggttacaa ggaagtaaaa aaggatctca taattaaact
     5521 accaaagtat agtctgtttg agttagaaaa tggccgaaaa cggatgttgg ctagcgccgg
     5581 agagcttcaa aaggggaacg aactcgcact accgtctaaa tacgtgaatt tcctgtattt
     5641 agcgtcccat tacgagaagt tgaaaggttc acctgaagat aacgaacaga agcaactttt
     5701 tgttgagcag cacaaacatt atctcgacga aatcatagag caaatttcgg aattcagtaa
     5761 gagagtcatc ctagctgatg ccaatctgga caaagtatta agcgcataca acaagcacag
     5821 ggataaaccc atacgtgagc aggcggaaaa tattatccat ttgtttactc ttaccaacct
     5881 cggcgctcca gccgcattca agtattttga cacaacgata gatcgcaaac gatacacttc
     5941 taccaaggag gtgctagacg cgacactgat tcaccaatcc atcacgggat tatatgaaac
     6001 tcggatagat ttgtcacagc ttgggggtga cggatccccc aagaagaaga ggaaagtctc
     6061 gagcgactac aaagaccatg acggtgatta taaagatcat gacatcgatt acaaggatga
     6121 cgatgacaag gctgcaggag gcggtggaag cgggcgcgcc catatgttaa ttaacaatta
     6181 accggtcatc atcaccatca ccattgagtt taaacccgct gatcagcctc gactgtgcct
     6241 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     6301 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     6361 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     6421 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     6481 tggggctcga taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     6541 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     6601 tgtaaagcct agggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     6661 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     6721 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     6781 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     6841 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     6901 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     6961 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     7021 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     7081 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     7141 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     7201 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     7261 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     7321 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     7381 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     7441 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     7501 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     7561 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     7621 atccttttaa attaaaaatg aagttttaaa tca
//
