LOCUS       sequence-211603-        9319 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression plasmid of sgRNA with GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9319)
  AUTHORS   Tarumoto Y, Lu B, Somerville TDD, Huang YH, Milazzo JP, Wu XS, 
            Klingbeil O, El Demerdash O, Shi J, Vakoc CR
  TITLE     LKB1, Salt-Inducible Kinases, and MEF2C Are Linked Dependencies in 
            Acute Myeloid Leukemia.
  JOURNAL   Mol Cell. 2018 Feb 28. pii: S1097-2765(18)30109-6. doi: 
            10.1016/j.molcel.2018.02.011.
  PUBMED    29526696
REFERENCE   2  (bases 1 to 9319)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2018 Feb 28. pii: S1097-2765(18)30109-6. doi: 
            10.1016/j.molcel.2018.02.011."
COMMENT     Sequence Label: LRG2.1
FEATURES             Location/Qualifiers
     source          1..9319
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        7..233
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             234..414
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    461..586
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1079..1312
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1497..1541
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1719..1967
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1719..1739
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     1890..1909
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    3993..4110
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4179..4390
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      4410..4419
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4419..4442
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(4443..4445,4446..4448,4449..5162)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4443..4445,4446..4448,4449..5159)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4488..4509)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4749..4768)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5096..5117
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5178..5766
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5231..5251)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5649..5660)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5838..6071
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6149..6270
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6186..6205)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6240..6259
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6310..6445
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6465..6484)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6466..6484)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6494..6511)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6494..6510)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6503..6525)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6652..7107
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6739..6758)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6949..6970
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7133..7237
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7238..7306,7307..8098)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7456..7475)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8269..8857
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8758..8777
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9011..9028
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9145..9166
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9181..9186,9187..9204,9205..9211)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9219..9235
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9224..9246
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9243..9259
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9243..9259
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9278..9298
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9280..9298
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc
       61 aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt
      121 acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg
      181 aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
      481 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
      541 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
      601 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
      661 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
      721 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
      781 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
      841 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
      901 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
      961 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     1021 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     1081 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     1141 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     1201 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     1261 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     1321 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     1381 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     1441 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     1501 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     1561 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     1621 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     1681 cgtttcagac ccacctccca accccgaggg gacccagaga gggcctattt cccatgattc
     1741 cttcatattt gcatatacga tacaaggctg ttagagagat aattagaatt aatttgactg
     1801 taaacacaaa gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt
     1861 ttgcagtttt aaaattatgt tttaaaatgg actatcatat gcttaccgta acttgaaagt
     1921 atttcgattt cttggcttta tatatcttgt ggaaaggacg aaacaccgga gacggttgta
     1981 aatgagcaca caaaatacac atgctaaaat attatattct atgaccttta taaaatcaac
     2041 caaaatcttc tttttaataa ctttagtatc aataattaga atttttatgt tcctttttgc
     2101 aaacttttaa taaaaatgag caaaataaaa aaacgctagt tttagtaact cgcgttgttt
     2161 tcttcacctt taataatagc tactccacca cttgttccta agcggtcagc tcctgcttca
     2221 atcatttttt gagcatcttc aaatgttcta actccaccag ctgctttaac taaagcattg
     2281 tctttaacaa ctgacttcat tagtttaaca tcttcaaatg ttgcacctga ttttgaaaat
     2341 cctgttgatg ttttaacaaa ttctaatcca gcttcaacag ctatttcaca agctttcatg
     2401 atttcttctt ttgttaataa acaattttcc ataatacatt taacaacatg tgatccagct
     2461 gcttttttta cagctttcat gtcttctaaa actaattcat aatttttgtc ttttaatgca
     2521 ccaatattta ataccatatc aatttctgtt gcaccatctt taattgcttc agaaacttcg
     2581 aatgcttttg tagctgttgt gcatgcacct agaggaaaac ctacaacatt tgttattcct
     2641 acatttgtgc cttttaataa ttctttacaa tagcttgttc aatatgaatt aacacaaact
     2701 gttgcaaaat caaattcaat tgcttcatca cataattgtt taatttcagc tttcgtagca
     2761 tcttgtttta ataatgtgtg atctatatat ttgtttagtt tcattttttc tcctatatat
     2821 tcatttttaa ttttaattct ttaataattt cgtctacttt aactttagcg ttttgaacag
     2881 attcaccaac acctataaaa taaattttta gtttaggttc agttccactt gggcgaacag
     2941 caaatcatga cttatcttct aaataaaatt ttagtaagtc ttgtcctggc atattataca
     3001 ttccatcgat gtagtcttca acattaacaa ctttaagtcc agcaatttga gttaagggtg
     3061 ttgctctcaa tgatttcatt aatggttcaa tttttaattt cttttcttct ggtttaaaat
     3121 tcaagtttaa agtgaaagtg taatatgcac ccatttcttt aaataaatct tctaaatagt
     3181 ctactaatgt tttattttgt tttttataaa atcaagcagc ctctgctatt aatatagaag
     3241 cttgtattcc atctttatct ctagctgagt catcaattac atatccataa ctttcttcat
     3301 aagcaaaaac aaaatttaat ccgttatctt cttctttagc aatttctcta cccattcatt
     3361 taaatccagt taaagttttt acaatattaa ctccatattt ttcatgagcg attctatcac
     3421 ccaaatcact tgttacaaaa cttgaatata gagccggatt ttttggaatg ctatttaagc
     3481 gttttagatt tgataatttt caatcaatta aaattggtcc tgtttgattt ccatctaatc
     3541 ttacaaaatg accatcatgt tttattgcca ttccaaatct gtcagcatct gggtcattca
     3601 taataataat atctgcatca tgtttaatac catattcaag cggtattttt catgcaggat
     3661 caaattctgg atttggattt acaacatttt taaatgtttc atcttcaaat gcatgctctt
     3721 caacctcaat aacgttatat cctgattcac gtaatatttt tggggtaaat ttagttcctg
     3781 ttccattaac tgcgctaaaa ataattttta aatctttttt agcttcttgc tcttttttgt
     3841 acgtctctgt ttaagagcta tgctggaaac agcatagcaa gtttaaataa ggctagtccg
     3901 ttatcaactt tgctggaaac agcaaagtgg caccgagtcg gtgctttttt tgaattctag
     3961 atcttgagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     4021 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     4081 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     4141 ctttggcgcc ggctcgagtg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
     4201 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg
     4261 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
     4321 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
     4381 cagaacacag gtgtcgtgac gcgggatccg ccaccatgga ttacaaagac gatgacgata
     4441 agatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     4501 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     4561 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     4621 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     4681 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     4741 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     4801 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     4861 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     4921 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     4981 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     5041 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     5101 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     5161 agacgcgtta agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     5221 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     5281 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     5341 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     5401 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     5461 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     5521 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     5581 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     5641 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     5701 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     5761 ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta gccacttttt
     5821 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     5881 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     5941 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     6001 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     6061 aatctctagc agtacgtata gtagttcatg tcatcttatt attcagtatt tataacttgc
     6121 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     6181 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     6241 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     6301 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     6361 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     6421 tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt
     6481 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     6541 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     6601 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct
     6661 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     6721 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     6781 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     6841 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     6901 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     6961 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     7021 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     7081 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac
     7141 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     7201 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     7261 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     7321 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     7381 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     7441 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     7501 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     7561 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     7621 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     7681 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     7741 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     7801 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     7861 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     7921 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     7981 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     8041 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     8101 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     8161 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     8221 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     8281 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     8341 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     8401 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     8461 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     8521 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     8581 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     8641 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     8701 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     8761 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     8821 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     8881 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     8941 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     9001 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     9061 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     9121 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     9181 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     9241 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga
     9301 acaaaagctg gagctgcaa
//
