LOCUS       sequence-211612-        6594 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express mouse LRRC33 in 293T cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6594)
  AUTHORS   Qin Y, Garrison BS, Ma W, Wang R, Jiang A, Li J, Mistry M, Bronson 
            RT, Santoro D, Franco C, Robinton DA, Stevens B, Rossi DJ, Lu C, 
            Springer TA
  TITLE     A Milieu Molecule for TGF-beta Required for Microglia Function in 
            the Nervous System.
  JOURNAL   Cell. 2018 Jun 28;174(1):156-171.e16. doi: 
            10.1016/j.cell.2018.05.027. Epub 2018 Jun 14.
  PUBMED    29909984
REFERENCE   2  (bases 1 to 6594)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.05.027"; journalName: "Cell"; date: 
            "2018-06-28- 28"; volume: "174"; issue: "1"; pages: "156-171.e16"
COMMENT     Sequence Label: plexm-mouse LRRC33
FEATURES             Location/Qualifiers
     source          1..6594
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          133..1149
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1073..1095
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1157..1176
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1294..1317
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(3461..3480)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3526..3581
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3580..3599)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3942..3958)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3942..3958)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3955..3977)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3966..3982
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3990..3996,3997..4014,4015..4020))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4035..4056
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4185..4202)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4356..4944)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4436..4455)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5115..5906,5907..5975))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5738..5757
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5976..6080)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        6111..6490
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ggcggggggn ngggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg
       61 ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc
      121 gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc
      181 tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac
      241 ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg
      301 tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg
      361 gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc
      421 ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg
      481 agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc
      541 gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa
      601 ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc
      661 cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg
      721 ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc
      781 cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg
      841 tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg
      901 cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa
      961 atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct
     1021 cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc
     1081 ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt
     1141 ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa
     1201 ttgcggccgt ctcaggccga attcgccacc atgtggtggc gcctgtggtg gctgctgctg
     1261 ctgctgctgc tgctgtggcc catggtgtgg gccgactaca aggacgacga cgacaagctc
     1321 gagtggagga gcggacctgg gacagccact gcagcttccc aaggaggctg caaggtggtc
     1381 gacggagtcg ctgactgccg gggtctgaac cttgcttcgg tacccagcag cctcccaccc
     1441 cattcccgga tgctcattct ggatgctaac cctctcaagg acctgtggaa ccattccctc
     1501 caggcctacc cacgcctgga gaacctcagc ctgcatagct gtcacctgga ccgtatcagc
     1561 cactacgcct tccgagagca aggccactta cgcaacctgg ttctggcaga caaccgcctc
     1621 tcggagaact acaaggagtc agccgcagca ctccacaccc tgctgggact tcggagactg
     1681 gacttgtctg gaaactccct gaccgaagac atggcggctc tcatgcttca gaacctctcc
     1741 tcgttggagg ttgtgtcctt ggcaaggaat accctcatga ggctcgacga ctctatcttt
     1801 gagggcctgg aacaccttgt agagctagat ttgcagagaa actacatctt tgagatcgag
     1861 ggcggggcgt ttgacggctt gactgagctg aggcgtctca accttgccta taacaacctc
     1921 ccctgcattg tggacttcag cctcacccag ttacggttcc tcaacgtcag ttataacatc
     1981 ctagagtggt tcctggcagc cagggaggag gtggccttcg aattggagat actggacctg
     2041 tctcacaacc agctgctctt cttcccactc ttgcctcagt gcggcaagct gcacaccctc
     2101 ctgctccagg acaacaacat gggcttctac agggagcttt acaacacctc ctccccgcag
     2161 gagatggtgg cccagttcct tcttgtggac ggcaatgtga ctaacatcac aactgtcaac
     2221 ctctgggagg agttttcctc cagcgacctg tcagctcttc gcttcctgga catgagccaa
     2281 aaccagttcc ggcacttgcc ggatggtttc ctgaagaaga caccttccct ttcccacctg
     2341 aacctcaacc agaactgcct gaagatgctc cacatccggg agcacgagcc gccgggagca
     2401 ctcaccgagc tggatctgag ccacaaccag ttggcagagc tgcacctggc tccggggctc
     2461 acgggctccc tcaggaacct ccgggtgttc aacctgagtt ccaaccagct cctgggggtc
     2521 cccactggcc ttttcgacaa tgccagcagc atcactacaa tcgacatgag tcacaatcag
     2581 atctcacttt gtcctcagat ggtgcccgta gactgggagg ggcctcccag ttgtgtggat
     2641 ttcagaaaca tgggctcttt gaggagcctt tctctggacg gctgtgggct gaaggcatta
     2701 caagactgcc cgttccaggg gacctccctc actcatttag acctgtctag caactggggg
     2761 gttctgaatg ggagcatcag ccctctctgg gctgttgccc cgacattaca ggtcctgtct
     2821 ctcagggacg tgggccttgg ttctggcgct gcagagatgg acttctctgc gtttgggaat
     2881 ctgcgggcgt tggatctgtc gggaaactcc ctgaccagct tcccaaagtt caagggcagt
     2941 ttggcccttc ggactctcga cctccgcaga aactctctca cggccctccc tcagagggtt
     3001 gtgtccgagc agcctctgag gggtctgcag accatctacc tcagccagaa cccttatgac
     3061 tgctgtgggg tggaaggatg gggggccctg cagcagcact tcaagactgt tgcggacttg
     3121 tccatggtca cttgcaacct ctcttccaag atcgtccgtg tggtggagct gcccgaaggc
     3181 ctgcctcagg gctgtaagtg ggaacaggtg gacactggtc tcttctacct cgtgctcatc
     3241 ctgcccagct gcctcaccct gctggtggcc tgtactgtcg tcttcctcac ttttaagaag
     3301 cctttgcttc aggtcatcaa gagccgctgc cactggtcct ccatatacac cggttaaagt
     3361 cgagactagt atcgcgataa ttcactcctc aggtgcaggc tgcctatcag aaggtggtgg
     3421 ctggtgtggc caatgccctg gctcacaaat accactgaga tctttttccc tctgccaaaa
     3481 attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta
     3541 ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga
     3601 gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc
     3661 atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat atgaaacagc
     3721 cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta
     3781 tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac
     3841 tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat
     3901 ggagatccct cgacctgcag cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg
     3961 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     4021 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     4081 ttccagtcgg gaaacctgtc gtgccagcgg atcgatccgc tgcattaatg aatcggccaa
     4141 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     4201 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     4261 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     4321 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     4381 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     4441 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     4501 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     4561 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     4621 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     4681 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     4741 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     4801 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     4861 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     4921 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     4981 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     5041 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     5101 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     5161 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     5221 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     5281 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     5341 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     5401 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     5461 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     5521 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     5581 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     5641 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     5701 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     5761 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     5821 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     5881 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     5941 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     6001 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     6061 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgggtc gacattgatt
     6121 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     6181 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     6241 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     6301 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     6361 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     6421 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     6481 tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc
     6541 cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga tggg
//
