LOCUS       sequence-211613-        6622 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mouse LRRC33 in 293T cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6622)
  AUTHORS   Qin Y, Garrison BS, Ma W, Wang R, Jiang A, Li J, Mistry M, Bronson 
            RT, Santoro D, Franco C, Robinton DA, Stevens B, Rossi DJ, Lu C, 
            Springer TA
  TITLE     A Milieu Molecule for TGF-beta Required for Microglia Function in 
            the Nervous System.
  JOURNAL   Cell. 2018 Jun 28;174(1):156-171.e16. doi: 
            10.1016/j.cell.2018.05.027. Epub 2018 Jun 14.
  PUBMED    29909984
REFERENCE   2  (bases 1 to 6622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.05.027"; journalName: "Cell"; date: 
            "2018-06-28- 28"; volume: "174"; issue: "1"; pages: "156-171.e16"
COMMENT     Sequence Label: plexm-mouse LRRC33
FEATURES             Location/Qualifiers
     source          1..6622
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        73..376
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        378..655
                     /label=chicken beta-actin promoter
     intron          656..1672
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1596..1618
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1680..1699
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1817..1840
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(3984..4003)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4049..4104
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4103..4122)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4465..4481)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4465..4481)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4478..4500)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4489..4505
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4513..4519,4520..4537,4538..4543))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4558..4579
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4708..4725)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4879..5467)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4959..4978)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5638..6429,6430..6498))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6261..6280
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6499..6603)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
       61 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      121 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      181 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      241 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      301 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      361 catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc
      421 cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg
      481 ggcggggggg gggggggggc gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg
      541 ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt
      601 tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcgggagt
      661 cgctgcgcgc tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg
      721 gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg
      781 ctgtaattag cgcttggttt aatgacggct tgtttctttt ctgtggctgc gtgaaagcct
      841 tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg cgtgcgtgtg
      901 tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg agcgctgcgg
      961 gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg ccgggggcgg
     1021 tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt
     1081 gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc tgcacccccc
     1141 tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg ggcgtggcgc
     1201 ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc
     1261 gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg ccggcggctg
     1321 tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg
     1381 acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc
     1441 tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct
     1501 tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct gtccgcgggg
     1561 ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg
     1621 cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg
     1681 caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgcggc cgtctcaggc
     1741 cgaattcgcc accatgtggt ggcgcctgtg gtggctgctg ctgctgctgc tgctgctgtg
     1801 gcccatggtg tgggccgact acaaggacga cgacgacaag ctcgagtgga ggagcggacc
     1861 tgggacagcc actgcagctt cccaaggagg ctgcaaggtg gtcgacggag tcgctgactg
     1921 ccggggtctg aaccttgctt cggtacccag cagcctccca ccccattccc ggatgctcat
     1981 tctggatgct aaccctctca aggacctgtg gaaccattcc ctccaggcct acccacgcct
     2041 ggagaacctc agcctgcata gctgtcacct ggaccgtatc agccactacg ccttccgaga
     2101 gcaaggccac ttacgcaacc tggttctggc agacaaccgc ctctcggaga actacaagga
     2161 gtcagccgca gcactccaca ccctgctggg acttcggaga ctggacttgt ctggaaactc
     2221 cctgaccgaa gacatggcgg ctctcatgct tcagaacctc tcctcgttgg aggttgtgtc
     2281 cttggcaagg aataccctca tgaggctcga cgactctatc tttgagggcc tggaacacct
     2341 tgtagagcta gatttgcaga gaaactacat ctttgagatc gagggcgggg cgtttgacgg
     2401 cttgactgag ctgaggcgtc tcaaccttgc ctataacaac ctcccctgca ttgtggactt
     2461 cagcctcacc cagttacggt tcctcaacgt cagttataac atcctagagt ggttcctggc
     2521 agccagggag gaggtggcct tcgaattgga gatactggac ctgtctcaca accagctgct
     2581 cttcttccca ctcttgcctc agtgcggcaa gctgcacacc ctcctgctcc aggacaacaa
     2641 catgggcttc tacagggagc tttacaacac ctcctccccg caggagatgg tggcccagtt
     2701 ccttcttgtg gacggcaatg tgactaacat cacaactgtc aacctctggg aggagttttc
     2761 ctccagcgac ctgtcagctc ttcgcttcct ggacatgagc caaaaccagt tccggcactt
     2821 gccggatggt ttcctgaaga agacaccttc cctttcccac ctgaacctca accagaactg
     2881 cctgaagatg ctccacatcc gggagcacga gccgccggga gcactcaccg agctggatct
     2941 gagccacaac cagttggcag agctgcacct ggctccgggg ctcacgggct ccctcaggaa
     3001 cctccgggtg ttcaacctga gttccaacca gctcctgggg gtccccactg gccttttcga
     3061 caatgccagc agcatcacta caatcgacat gagtcacaat cagatctcac tttgtcctca
     3121 gatggtgccc gtagactggg aggggcctcc cagttgtgtg gatttcagaa acatgggctc
     3181 tttgaggagc ctttctctgg acggctgtgg gctgaaggca ttacaagact gcccgttcca
     3241 ggggacctcc ctcactcatt tagacctgtc tagcaactgg ggggttctga atgggagcat
     3301 cagccctctc tgggctgttg ccccgacatt acaggtcctg tctctcaggg acgtgggcct
     3361 tggttctggc gctgcagaga tggacttctc tgcgtttggg aatctgcggg cgttggatct
     3421 gtcgggaaac tccctgacca gcttcccaaa gttcaagggc agtttggccc ttcggactct
     3481 cgacctccgc agaaactctc tcacggccct ccctcagagg gttgtgtccg agcagcctct
     3541 gaggggtctg cagaccatct acctcagcca gaacccttat gactgctgtg gggtggaagg
     3601 atggggggcc ctgcagcagc acttcaagac tgttgcggac ttgtccatgg tcacttgcaa
     3661 cctctcttcc aagatcgtcc gtgtggtgga gctgcccgaa ggcctgcctc agggctgtaa
     3721 gtgggaacag gtggacactg gtctcttcta cctcgtgctc atcctgccca gctgcctcac
     3781 cctgctggtg gcctgtactg tcgtcttcct cacttttaag aagcctttgc ttcaggtcat
     3841 caagagccgc tgccactggt cctccatata caccggttaa agtcgagact agtatcgcga
     3901 taattcactc ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc
     3961 ctggctcaca aataccactg agatcttttt ccctctgcca aaaattatgg ggacatcatg
     4021 aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg
     4081 tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa
     4141 catcagaatg agtatttggt ttagagtttg gcaacatatg cccatatgct ggctgccatg
     4201 aacaaaggtt ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc
     4261 ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta
     4321 tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc
     4381 ctctcctgac tactcccagt catagctgtc cctcttctct tatggagatc cctcgacctg
     4441 cagcccaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     4501 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     4561 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     4621 gtcgtgccag cggatcgatc cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     4681 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     4741 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     4801 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     4861 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     4921 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     4981 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     5041 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     5101 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     5161 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     5221 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     5281 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     5341 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     5401 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     5461 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     5521 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     5581 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     5641 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     5701 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     5761 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     5821 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     5881 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     5941 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     6001 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     6061 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     6121 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     6181 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     6241 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     6301 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     6361 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     6421 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     6481 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     6541 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     6601 gcgcacattt ccccgaaaag tg
//
