LOCUS       sequence-211615-        6642 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express human LRRC33 in 393T cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6642)
  AUTHORS   Qin Y, Garrison BS, Ma W, Wang R, Jiang A, Li J, Mistry M, Bronson 
            RT, Santoro D, Franco C, Robinton DA, Stevens B, Rossi DJ, Lu C, 
            Springer TA
  TITLE     A Milieu Molecule for TGF-beta Required for Microglia Function in 
            the Nervous System.
  JOURNAL   Cell. 2018 Jun 28;174(1):156-171.e16. doi: 
            10.1016/j.cell.2018.05.027. Epub 2018 Jun 14.
  PUBMED    29909984
REFERENCE   2  (bases 1 to 6642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.05.027"; journalName: "Cell"; date: 
            "2018-06-28- 28"; volume: "174"; issue: "1"; pages: "156-171.e16"
COMMENT     Sequence Label: plexm-human LRRC33
FEATURES             Location/Qualifiers
     source          1..6642
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        12..391
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        393..670
                     /label=chicken beta-actin promoter
     intron          671..1687
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1611..1633
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1695..1714
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1832..1855
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(4004..4023)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4069..4124
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4123..4142)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4485..4501)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4485..4501)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4498..4520)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4509..4525
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4533..4539,4540..4557,4558..4563))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4578..4599
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4728..4745)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4899..5487)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4979..4998)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5658..6449,6450..6518))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6281..6300
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6519..6623)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctgggt cgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
       61 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
      121 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
      181 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
      241 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
      301 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
      361 catctacgta ttagtcatcg ctattaccat ggtcgaggtg agccccacgt tctgcttcac
      421 tctccccatc tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt
      481 ttgtgcagcg atgggggcgg gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg
      541 cgaggggcgg ggcggggcga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct
      601 ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc
      661 gcggcgggcg ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc
      721 gcgccgcccg ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg
      781 cccttctcct ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg
      841 gctgcgtgaa agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg
      901 gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg
      961 ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag
     1021 cgcggccggg ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt
     1081 gcggggtgtg tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc
     1141 ccccctgcac ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg
     1201 tacggggcgt ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc
     1261 gggcggggcg gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggcccccg
     1321 gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg
     1381 cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg
     1441 ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat
     1501 gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg
     1561 gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt
     1621 ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt
     1681 cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt
     1741 gcggccgtct caggccgaat tcgccaccat gtggtggcgc ctgtggtggc tgctgctgct
     1801 gctgctgctg ctgtggccca tggtgtgggc cgactacaag gacgacgacg acaagctcga
     1861 ctggaggaac agaagcggaa cagccacagc agcctcccaa ggagtctgca agttggtggg
     1921 tggagccgct gactgccgag ggcagagcct cgcttcggtg cccagcagcc tcccgcccca
     1981 cgcccggatg ctcaccctgg atgccaaccc tctcaagacc ctgtggaatc actccctcca
     2041 gccttaccct ctcctggaga gcctcagcct gcacagctgc cacctggagc gcatcagccg
     2101 cggcgccttc caggagcaag gtcacctgcg cagcctggta ctgggggaca actgcctctc
     2161 agagaactac gaagagacgg cagccgccct ccacgccctg ccgggcctgc ggaggctgga
     2221 cttgtcagga aacgccctga cggaggacat ggcagcgctc atgctccaga acctctcctc
     2281 gctgcggtcc gtgtccctgg cggggaacac catcatgcgg ctggacgact ccgtcttcga
     2341 gggcctggag cgtctccggg agctggatct gcagaggaac tacatcttcg agatcgaggg
     2401 cggcgctttc gacggcctgg ctgagctgag gcacctcaac ctggccttca acaacctccc
     2461 ctgcatcgtg gacttcgggc tcacgcggct gcgggtcctc aacgtcagct acaacgtcct
     2521 ggagtggttc ctcgcgaccg ggggagaggc tgccttcgag ctggagacgc tggacctgtc
     2581 tcacaaccag ctgctgttct tcccgctgct gccccagtac agcaagttgc ggaccctcct
     2641 gctgcgcgac aacaacatgg gcttctaccg ggacctgtac aacacctcgt cgccgaggga
     2701 gatggtggcc cagttcctcc tcgtggacgg caacgtgacc aacatcacca ccgtcagcct
     2761 ctgggaagaa ttctcctcca gcgacctcgc agatctccgc ttcctggaca tgagccagaa
     2821 ccagttccag tacctgccag acggcttcct gaggaaaatg ccttccctct cccacctgaa
     2881 cctccaccag aattgcctga tgacgcttca cattcgggag cacgagcccc ccggagcgct
     2941 caccgagctg gacctgagcc acaaccagct gtcggagctg cacctggctc cggggctggc
     3001 cagctgcctg ggcagcctgc gcttgttcaa cctgagctcc aaccagctcc tgggcgtccc
     3061 ccctggcctc ttcgccaatg ctaggaacat cactacactt gacatgagcc acaatcagat
     3121 ctcactttgt cccctgccag ctgcctcgga ccgggtgggc ccccctagct gtgtggattt
     3181 caggaatatg gcatctttaa ggagcctgtc tctggagggc tgtggcctgg gggcattgcc
     3241 agactgccca ttccaaggga cctccctgac ctacttagac ctctcaagca actggggggt
     3301 tctgaatggg agcctcgccc cactccagga tgttgccccc atgttacagg tcctgtctct
     3361 caggaacatg ggcctccact ccagctttat ggcgttggac ttctctgggt ttgggaatct
     3421 cagggactta gatctgtcgg ggaattgctt gaccaccttc ccaaggtttg ggggcagcct
     3481 ggccctggag accctggatc tccgtagaaa ctcgctcaca gcccttcccc agaaggctgt
     3541 gtctgagcag ctctcgagag gtctgcggac catctacctc agtcagaatc catatgactg
     3601 ctgtggggtg gatggctggg gggccctgca gcatgggcag acggtggccg actgggccat
     3661 ggtcacctgc aacctctcct ccaagatcat ccgcgtgacg gagctgcccg gaggtgtgcc
     3721 tcgggactgc aagtgggagc ggctggacct gggcctgctc tacctcgtgc tcatcctccc
     3781 cagctgcctc accctgctgg tggcctgcac tgtcatcgtc ctcactttta agaagcctct
     3841 gcttcaggtc atcaagagcc gctgccactg gtcctccgtt tactgacctg gaccggttaa
     3901 agtcgagact agtatcgcga taattcactc ctcaggtgca ggctgcctat cagaaggtgg
     3961 tggctggtgt ggccaatgcc ctggctcaca aataccactg agatcttttt ccctctgcca
     4021 aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat
     4081 ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg
     4141 ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg
     4201 cccatatgct ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac
     4261 agccccctgc tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt
     4321 ttatattttg ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt
     4381 tactagccag atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct
     4441 tatggagatc cctcgacctg cagcccaagc ttggcgtaat catggtcata gctgtttcct
     4501 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     4561 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     4621 gctttccagt cgggaaacct gtcgtgccag cggatcgatc cgctgcatta atgaatcggc
     4681 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     4741 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     4801 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     4861 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     4921 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     4981 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     5041 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     5101 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     5161 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     5221 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     5281 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     5341 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     5401 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     5461 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     5521 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     5581 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     5641 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     5701 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     5761 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     5821 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     5881 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     5941 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     6001 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     6061 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     6121 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     6181 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     6241 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     6301 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     6361 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     6421 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     6481 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     6541 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     6601 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tg
//
