LOCUS       sequence-21171-e         742 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Sharma N, Low SH, Misra S, Pallavi B, Weimbs T
  TITLE     Apical targeting of syntaxin 3 is essential for epithelial cell 
            polarity.
  JOURNAL   J Cell Biol. 2006 Jun 19. 173(6):937-48.
  PUBMED    16785322
REFERENCE   2  (bases 1 to 742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2006 Jun 19. 173(6):937-48."
COMMENT     Sequence Label: Syn3D146
FEATURES             Location/Qualifiers
     source          1..742
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             259..270
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             559..588
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             598..627
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             643..660
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(683..700)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccggactc tagcgtttaa
       61 acttaagctt ggtaccgagc tcggatccac tagtccagtg tggtggaatt cagcatgggg
      121 cgaatccagc ggcagctcga aattactggc aaaaagacaa ccgatgagga gctggaggag
      181 atgttggaga gtggcaaccc ggccatcttc acttctggga tcattgactc acagatttcc
      241 aagcaagccc tcagtgagat tgagggacga cacaaggaca ttgtgaggct ggagagcagc
      301 atcaaggagc ttcacgacat gtttatggac atcgccatgc tggtggagaa tcagggtgag
      361 atgttagata acatagagtt gaatgtcatg cacacagtgg accacgtgga gaaggcacga
      421 gatgaaacga aaaaagctgt gaaataccag agtcaggccc ggaagaaatt gataattatc
      481 attgtgctag tagttgtgtt gctgggcatt ttagcattga ttattggact ttccgttggg
      541 ctgaatgcgg ccgctcgaga acaaaaactc atctcagaag aggatctggg gcccttcgaa
      601 caaaaactca tctcagaaga ggatctgaat atgcataccg gtcatcatca ccatcaccat
      661 tgagtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
      721 tgcccctccc ccgtgccttc ct
//
