LOCUS       sequence-211720-        5067 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR donor plasmid to tag human transcription factor TEAD1 with 
            GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5067)
  TITLE     White lab ENCODE plasmids to tag human transcription factors with 
            GFP
REFERENCE   2  (bases 1 to 5067)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pTEAD1-donor
FEATURES             Location/Qualifiers
     source          1..5067
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      31..619
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     520..539
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1675..1695
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1708..1752
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             1792..1815
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             join(1843..1845,1846..1848,1849..2562)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLK
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1888..1909)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2149..2168)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    join(2600..3173,3174..3176,3177..3185)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2766..2783)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2993..3012
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3267..4070
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3330..3349)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3940..3959
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
       61 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
      121 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
      181 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
      241 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
      301 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
      361 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      421 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      481 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      541 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      601 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacgggaaa ggagtcaggg
      661 tctgtgtggt gtggagggag ctgcaatgat ttgggctgga gaactcaaaa tacgagggat
      721 aattagaggg attcaagaga ctggagggga agcatgagta tttcacaata cattttgaaa
      781 gataaagccc atgtctatgg aaagaatgtc attccacagg attctaccta tcccactgtt
      841 ccttgattca gcagatactt ttctgggtct aggcactact ggttaacata gtggtgaggg
      901 aggcactcct gcagcttctc tgtccttggc tattgtgaaa caagatttag cagaagttta
      961 agactaggat gggaaaaaga gtggatttta aatgttctca tcacaaagaa gtacgtgaag
     1021 tgcttggtat gttagcctga tgtaatcgtc ccacaaggta tatacatcta tcatcacatt
     1081 gtagcccata aatatatgta ctgcaagact ttattctttt aggtagggac tggaattatt
     1141 acttgtcaat ttaaaaattc agataaaaaa taagacaatt tcttagagta aatgattgcc
     1201 ttgcgggaca gaattaaaga gaattctttg ttgtaaagaa atcagccccc taaaaaacaa
     1261 aaacacaaat gtgctaattt ttgtggtcct ttcttgggca tagattatga gtgaccctat
     1321 ctagaaggta tttcttactt atgataacca cgtttagatg ggcttcaggt catagttgta
     1381 gtgttctgaa tctgtgttag aggacacaga gtgaggctga gcaaatctct cttggactta
     1441 agacttgttc ttctccaatt aagtcatagt cgtcatacac agatggaata tgttttcatc
     1501 cttttggtat tatatacttt ttttttatct taacaggtgg taacaaacag ggatacacaa
     1561 gaaactctac tctgcatggc ctgtgtgttt gaagtttcaa atagtgaaca cggagcacaa
     1621 catcatattt acaggcttgt aaaggacgat tatgatattc caactactgc aagcgagaat
     1681 ctttattttc agggcgccgc caaattcaaa gaaaccgctg ctgctaaatt cgaacgccag
     1741 cacatggaca gcggaggtgg aggttcgagc ggtccctcgg gttcgtcgag cctggaagtt
     1801 ctgttccagg ggcccctgtc gtcgagcggt ccctcgggtt cgatggtgag caaaggcgag
     1861 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     1921 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     1981 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
     2041 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     2101 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     2161 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     2221 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     2281 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     2341 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     2401 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     2461 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tccttaagga gttcgtgacc
     2521 gccgccggga tcactctcgg catggacgag ctgtacaagt aatgatctag agtcgagtta
     2581 attaagaatt ccgccccccc ccctctccct ccccccccct aacgttactg gccgaagccg
     2641 cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt
     2701 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct
     2761 ttcccctctc gccaaaggaa tgcagggtct gttgaatgtc gtgaaggaag cagttcctct
     2821 ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc
     2881 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc
     2941 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc
     3001 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc
     3061 tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct
     3121 aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca
     3181 caacctgtta cattgcacaa gataaaaata tatcatcacg aacagtaaaa ctgtctgctt
     3241 acataaacag taatacaagg ggtgttatgg gatcggccat tgaacaagat ggattgcacg
     3301 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     3361 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     3421 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     3481 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     3541 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     3601 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     3661 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     3721 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     3781 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
     3841 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     3901 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     3961 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     4021 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga acatggttat
     4081 ttatatatat agatatctgt atatacacac acacatatgt gcacacacac actctctctc
     4141 cattatcgaa cgactgactg taaacctcac cacacagggt ggtgccctgg ccccgaggtc
     4201 accccgactt ttctaaatct tgtttgagtg aagtcatttt ttcatgtgtt catactatca
     4261 ttgtagctgt gaagttctgg tacagttgta aaaagagaaa ttgagttgtt tctctatgtt
     4321 cttcagatgt gcagcccaca attcctcggg aaaggtgaac ctgaacaacc caagtctctc
     4381 tctgcagagc cctgtttcta attgtggtag aaaatattga gacagagcat ttgccatggg
     4441 acatttacag cctttataca aatgtattta gttctctttt ttccaacata aaattcttgt
     4501 tttaagatac aagtaaaatt aatctttaaa tataaatgta aattagtaca caaaactaag
     4561 aatctttaga cttatctttg taactaatta gggtggaagt tatgaaagaa tgtaattcac
     4621 taaattattt tttaaatgaa accttttttt ttctttttga aaccaaatgt taaactatag
     4681 ccttaagaaa tgcttggtag aagtgtccta atgagacaaa tttgtacttt tatcctcaag
     4741 gttaacacta atctcctaat ccattaaact cttgaacagg tattacaaag gaagaaaact
     4801 tcacccctta tccttaacat atatagtata tttaaaaaat ataaaattgt attgtactaa
     4861 tgtgatgatg gattatttaa tgaaaaagaa aaaatggctc tttttgcaat aagtagatac
     4921 atactgaaaa aatctaaact tacaatgttt atagtcttgt gtgtgcagtt atattttata
     4981 tggacgacca aattttttat taagatgagt aaatatttga accactgaat tttaataaca
     5041 aaattttaaa attggcatga atacgga
//
