LOCUS       sequence-21181-e         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Kurosu T, Peterlin BM
  TITLE     VP16 and ubiquitin; binding of P-TEFb via its activation domain and 
            ubiquitin facilitates elongation of transcription of target genes.
  JOURNAL   Curr Biol. 2004 Jun 22. 14(12):1112-6.
  PUBMED    15203006
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            2004 Jun 22. 14(12):1112-6."
COMMENT     Sequence Label: pCMV Ub Lex HA (P#1707)
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             95..316
                     /codon_start=1
                     /product="Saccharomyces cerevisiae ubiquitin"
                     /label=ubiquitin
                     /translation="QIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFA
                     GKQLEDGRTLSDYNIQKESTLHLVLRLRG"
     CDS             335..367
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             374..628
                     /codon_start=1
                     /gene="lexA (truncated)"
                     /product="DNA-binding domain of E. coli LexA"
                     /label=LexA BD
                     /note="binds the 16-bp palindromic sequence 
                     5'-CTGTATATATATACAG-3'"
                     /translation="MAKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAA"
ORIGIN
        1 cgtgggnntt agcgactctc tggctactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tttgcagatc tttgtgaaga ccttgaccgg
      121 taaaaccata acattggaag ttgaatcttc cgataccatc gacaacgtta agtcgaaaat
      181 tcaagacaag gaaggtatcc ctccagatca acaaagattg atctttgccg gtaagcagct
      241 agaagacggt agaacgctgt ctgattacaa cattcagaag gagtccacct tacatcttgt
      301 gctaaggctg agaggtgtcg tcggtaagaa cggtatggct agcatgactg gtggacagca
      361 aatgggtaag gccatggcaa aagcgttaac ggccaggcaa caagaggtgt ttgatctcat
      421 ccgtgatcac atcagccaga caggtatgcc gccgacgcgt gcggaaatcg cgcagcgttt
      481 ggggttccgt tccccaaacg cggctgaaga acatctgaag gcgctggcac gcaaaggcgt
      541 tattgaaatt gtttccggcg catcacgcgg gattcgtctg ttgcaggaag aggaagaagg
      601 gttgccgctg gtaggtcgtg tggctgccgg tgaaccactt ctggcgcaac agcatattga
      661 aggtcattat caggtcgatc cttccttatt tcaagccgaa tgctgatttc cggctgccgt
      721 cagcggctgt cnatnaaaaa tatcgncntt atggnatggt gactngctgg cagtgcataa
      781 aactcaggat gtacttacgg tcaggtcgtt gt
//
