LOCUS       sequence-211882-        5528 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Indicator for glutamate imaging (synapsin promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5528)
  AUTHORS   Helassa N, Durst CD, Coates C, Kerruth S, Arif U, Schulze C, Wiegert
            JS, Geeves M, Oertner TG, Torok K
  TITLE     Ultrafast glutamate sensors resolve high-frequency release at 
            Schaffer collateral synapses.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 May 7. pii: 1720648115. doi: 
            10.1073/pnas.1720648115.
  PUBMED    29735711
REFERENCE   2  (bases 1 to 5528)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2018 May 7. pii: 1720648115. doi: 
            10.1073/pnas.1720648115."
COMMENT     Sequence Label: pCI syn iGluSnFR
FEATURES             Location/Qualifiers
     source          1..5528
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        61..508
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     intron          545..677
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     660..679
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        722..740
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     sig_peptide     831..890
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     primer_bind     1859..1880
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1986..2007)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2247..2266)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             2472..2501
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2505..2651
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     polyA_signal    2678..2799
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2689..2708)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2743..2762
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      2980..3435
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3067..3086)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3277..3298
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3502..3521)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3621..3643
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3681..3699)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3767..3871
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3872..3940,3941..4732)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4090..4109)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4903..5491
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5392..5411
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tttgctggcc ttttgctcac atggctcgac agatctaatc tgcagagggc cctgcgtatg
       61 agtgcaagtg ggttttaaga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga
      121 ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc ccctatcaga
      181 gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg
      241 gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc
      301 gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg
      361 cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg
      421 cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg
      481 aggagtcgtg tcgtgcctga gagcgcagct gcagctagga agttggtcgt gaggcactgg
      541 gcaggtaagt atcaaggtta caagacaggt ttaaggagac caatagaaac tgggcttgtc
      601 gagacagaga agactcttgc gtttctgata ggcacctatt ggtcttactg acatccactt
      661 tgcctttctc tccacaggtg tccactccca gttcaattac agctcttaag gctagagtac
      721 ttaatacgac tcactatagg ctagcgctac cggactcaag cttggtaccg aactcggatc
      781 cactagtaac ggccgccagt gtgctggaat tcggcttggg gatatccacc atggagacag
      841 acacactcct gctatgggta ctgctgctct gggttccagg ttccactggt gacagatctg
      901 ccgcaggcag cacgctggac aaaatcgcca aaaacggtgt gattgtcgtc ggtcaccgtg
      961 aatcttcagt gcctttctct tattacgaca atcagcaaaa agtggtgggt tactcgcagg
     1021 attactccaa cgccattgtt gaagcagtga aaaagaaact caacaaaccg gacttgcagg
     1081 taaaactgat tccgattacc tcacaaaacc gtattccact gctgcaaaac ggcactttcg
     1141 attttgaatg tggttctacc accaacaacg tcgaacgcca aaaacaggcg gctttctctg
     1201 acactatttt cgtggtcggt acgcgcctgt tgaccaaaaa gggtggcgat atcaaagatt
     1261 ttgccaacct gaaagacaaa gccgtagtcg tcacttccgg cactacctct gaagttttgc
     1321 tcaacaaact gaatgaagag caaaaaatga atatgcgcat catcagcgcc aaagatcacg
     1381 gtgactcttt ccgcaccctg gaaagcggtc gtgccgttgc ctttatgatg gatgacgtgc
     1441 tgctggccgg tgaacgtgcg aaagcgaaga aaccagacaa ctgggaaatc gtcggcaagc
     1501 cgcagtctca ggaggcctac ggttgtatgt tgcgtaaaga tgatccgcag ttcaaaaagc
     1561 tgatggatga caccatcgct caggtgcaga cctccggtga agcggaaaaa tggtttgata
     1621 agtggttcaa aaatccaatt ctggtgagcc acaacgtcta tatcatggcc gacaagcaga
     1681 ggaacggcat caaggcgaac ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc
     1741 tcgcctacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     1801 accactacct gagcacccag tccaaacttt cgaaagaccc caacgagaag cgcgatcaca
     1861 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     1921 agggcggtac cggagggagc atggtgagca agggcgagga gctgttcacc ggggtggtgc
     1981 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     2041 gtgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     2101 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
     2161 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctaca
     2221 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     2281 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     2341 acggcaacat cctggggcat aagctggagt acaactttaa caaccccctg aacatgaatt
     2401 tcgaactgtc agacgaaatg aaagcactgt tcaaagaacc gaatgacaag gcactgaaac
     2461 tgcaggtcga cgaacaaaaa ctcatctcag aagaggatct gaatgctgtg ggccaggaca
     2521 cgcaggaggt catcgtggtg ccacactcct tgccctttaa ggtggtggtg atctcagcca
     2581 tcctggccct ggtggtgctc accatcatct cccttatcat cctcatcatg ctttggcaga
     2641 agaagccacg ttaggcggcc gcttcgagca gacatgataa gatacattga tgagtttgga
     2701 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
     2761 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat
     2821 tttatgtttc aggttcaggg ggagatgtgg gaggtttttt aaagcaagta aaacctctac
     2881 aaatgtggta aaatcgataa ggatccgggc tggcgtaata gcgaagaggc ccgcaccgat
     2941 cgcccttccc aacagttgcg cagcctgaat ggcgaatgga cgcgccctgt agcggcgcat
     3001 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     3061 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     3121 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     3181 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     3241 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     3301 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     3361 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     3421 taacgcttac aatttcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     3481 ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
     3541 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     3601 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     3661 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     3721 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     3781 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     3841 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     3901 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     3961 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     4021 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     4081 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     4141 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     4201 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     4261 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     4321 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     4381 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     4441 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     4501 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     4561 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     4621 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     4681 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     4741 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     4801 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     4861 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     4921 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     4981 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     5041 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     5101 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     5161 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     5221 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     5281 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     5341 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     5401 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     5461 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     5521 cctggcct
//
