LOCUS       sequence-211887-         972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses channel rhodopsin in astrocytes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Octeau JC, Gangwani MR, Allam SL, Tran D, Huang S, Hoang-Trong TM, 
            Golshani P, Rumbell TH, Kozloski JR, Khakh BS
  TITLE     Transient, Consequential Increases in Extracellular Potassium Ions 
            Accompany Channelrhodopsin2 Excitation.
  JOURNAL   Cell Rep. 2019 May 21;27(8):2249-2261.e7. doi: 
            10.1016/j.celrep.2019.04.078.
  PUBMED    31116972
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.04"; journalName: "Cell Rep"; date: 
            "2019-05-21- 21"; volume: "27"; issue: "8"; pages: "2249-2261.e7"
COMMENT     Sequence Label: pZac2.1 GfaABC1D ChR2(H134R) mCherry SV40
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             166..873
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(308..326)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     576..595
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(924..943)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tgttgggggt tgttgggaca ctacctgcgc gtcctgatcc acgagcacat attgattcac
       61 ggagatatcc gcaaaaccac caaactgaac atcggcggaa cggagatcga ggtcgagact
      121 ctcgtcgaag acgaagccga ggccggagcc gtgccagcgg ccgccgtgag caagggcgag
      181 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
      241 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
      301 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
      361 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
      421 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
      481 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
      541 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
      601 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
      661 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac
      721 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc
      781 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
      841 tccaccggcg gcatggacga gctgtacaag taatctagag tcgacccggg cggccgcttc
      901 gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
      961 aaaaatgctt ta
//
