LOCUS       sequence-21189-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Kurosu T, Zhang F, Peterlin BM
  TITLE     Transcriptional activity and substrate recognition of cyclin T2 from
            P-TEFb.
  JOURNAL   Gene. 2004 Dec 8. 343(1):173-9.
  PUBMED    15563843
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene. 2004 
            Dec 8. 343(1):173-9."
COMMENT     Sequence Label: pEF myc Gal cycT2b (P#1719)
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             87..104
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             114..143
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             159..599
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
ORIGIN
        1 tctttcggtg tcgtgaggat tagcttgctt acatttgctt ctgacacaac tgtgttcact
       61 agcaacctca aacagacacc atgccgcatc accaccatca tcacgccatg ccggagcaga
      121 agctgatatc cgaggaggac ctggccatgg ccggatccat gaagctactg tcttctatcg
      181 aacaagcatg cgatatttgc cgacttaaaa agctcaagtg ctccaaagaa aaaccgaagt
      241 gcgccaagtg tctgaagaac aactgggagt gtcgctactc tcccaaaacc aaaaggtctc
      301 cgctgactag ggcacatctg acagaagtgg aatcaaggct agaaagactg gaacagctat
      361 ttctactgat ttttcctcga gaagaccttg acatgatttt gaaaatggat tctttacagg
      421 atataaaagc attgttaaca ggattatttg tacaagataa tgtgaataaa gatgccgtca
      481 cagatagatt ggcttcagtg gagactgata tgcctctaac attgagacag catagaataa
      541 gtgcgacatc atcatcggaa gagagtagta acaaaggtca aagacagttg actgtatcgc
      601 cggaattcgt cgacatggcg tcgggccgtg gagcttcttc tcgctggttc tttactcggg
      661 aacagctgga gaacacgccg agccgccgct gcggagtgga ggcggataaa gagctctcgt
      721 gccgccanca ggcgncaacc tcatccagga atgggacagc gtctcatgtc tctcagcttt
      781 gaaaacctgc atgtttngcc gtttttnncg ggtttntgcc catcctccna attcacaaaa
      841 nattcnttgc
//
