LOCUS       sequence-211891-        8914 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FANCD2_-_10068140.23-P1P2 sgRNA in pCR1068.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8914)
  AUTHORS   Richardson CD, Kazane KR, Feng SJ, Zelin E, Bray NL, Schafer AJ, 
            Floor SN, Corn JE
  TITLE     CRISPR-Cas9 genome editing in human cells occurs via the Fanconi 
            anemia pathway.
  JOURNAL   Nat Genet. 2018 Aug;50(8):1132-1139. doi: 10.1038/s41588-018-0174-0.
            Epub 2018 Jul 27.
  PUBMED    30054595
REFERENCE   2  (bases 1 to 8914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41588-018-0174-0"; journalName: "Nat Genet"; date: 
            "2018-08"; volume: "50"; issue: "8"; pages: "1132-1139"
COMMENT     Sequence Label: pCR1164
FEATURES             Location/Qualifiers
     source          1..8914
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        26..405
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        407..605
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     560..580
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             623..803
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    850..975
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1472..1705
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1890..1934
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2232..2349
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(2610..2643)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     2647..2667
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     promoter        2920..3131
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          3132..4070
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4027..4047
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4091..4687
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4091..4110)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4587..4607
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4697..4750
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      4757..4766
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4763..4765,4766..4768,4769..5482)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4808..4829)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5069..5088)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5416..5437
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(5502..5535)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    5591..6179
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5644..5664)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6062..6073)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6182..6198)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6183..6199)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6708..6888
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(6950..7538)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7030..7049)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7709..8500,8501..8569))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8332..8351
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8570..8674)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8749..8768)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca
       61 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
      121 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
      181 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
      241 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
      301 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
      361 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
      421 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
      481 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
      541 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      601 agcagcgcgt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct
      661 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
      721 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
      781 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
      841 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
      901 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
      961 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
     1021 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     1081 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     1141 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     1201 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     1261 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccggc
     1321 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     1381 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     1441 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     1501 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     1561 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     1621 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     1681 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     1741 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     1801 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     1861 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     1921 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     1981 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     2041 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     2101 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     2161 ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca aatggcagta
     2221 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     2281 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     2341 caaaattttc gggtttatta cagggacagc agagatccag tttggttagt accgggcccg
     2401 ccctgcaggt ctagagatcc gacgcgccat ctctaggccc gcgccggccc cctcgcacgg
     2461 acttgtggga gaagctcggc tactcccctg ccccggttaa tttgcatata atatttccta
     2521 gtaactatag aggcttaatg tgcgataaaa gacagataat ctgttctttt taatactagc
     2581 tacattttac atgataggct tggatttcta taacttcgta tagcatacat tatacgaagt
     2641 tataaacagc acaaaaggaa actcacccta actgtaaagt aattgtgtgt tttgagacta
     2701 taagtatccc ttggagaacc accttgttgg cgggaaagtc gaaaactacg tttaagagct
     2761 aagctggaaa cagcatagca agtttaaata aggctagtcc gttatcaact tgaaaaagtg
     2821 gcaccgagtc ggtgcttttt ttctcgagta ctaggatcca ttaggcggcc gccctgcagg
     2881 gtggataacc gtattaccgc catgcatgtg cccgtcagtg ggcagagcgc acatcgccca
     2941 cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc
     3001 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
     3061 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
     3121 ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg
     3181 gcccttgcgt gccttgaatt acttccacct ggctgcagta cgtgattctt gatcccgagc
     3241 ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc
     3301 gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc
     3361 ttcgcgcctg tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg
     3421 ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg
     3481 gtatttcggt ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt
     3541 cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg
     3601 gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa
     3661 ggctggcccg gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg
     3721 cagggagctc aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac
     3781 aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg
     3841 cgccgtccag gcacctcgat tagttctcgc gcttttggag tacgtcgtct ttaggttggg
     3901 gggaggggtt ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc
     3961 cagcttggca cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt
     4021 tcattctcaa gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgag
     4081 ctagcccacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     4141 ccgggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     4201 cgacccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     4261 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     4321 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     4381 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     4441 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     4501 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     4561 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     4621 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     4681 cggtgccgga tcgggagagg gcagaggaag tctgctaaca tgcggtgacg tcgaggagaa
     4741 tcctggccca ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
     4801 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     4861 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     4921 gctgccggtg ccctggccca ccctcgtgac caccctgacc catggcgtgc agtgcttcag
     4981 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     5041 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     5101 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     5161 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     5221 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     5281 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     5341 cgtgctgctg cccgacaacc actacctgag cacccagtcc aagctgagta aagaccccaa
     5401 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     5461 catggacgag ctgtacaagt aagaattcgt cgagggacct aataacttcg tatagcatac
     5521 attatacgaa gttatacatg tttaagggtt ccggttccac taggtacaat tcgatatcaa
     5581 gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     5641 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     5701 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     5761 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     5821 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     5881 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     5941 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     6001 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     6061 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     6121 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
     6181 tcgataccgt cgacctcgat cgagacctag aaaaacatgg agcaatcaca agtagcaata
     6241 cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt
     6301 ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct gtagatctta
     6361 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     6421 atatccttga tctgtggatc taccacacac aaggctactt ccctgattgg cagaactaca
     6481 caccagggcc agggatcaga tatccactga cctttggatg gtgctacaag ctagtaccag
     6541 ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa cacccgcttg ttacaccctg
     6601 tgagcctgca tgggatggat gacccggaga gagaagtatt agagtggagg tttgacagcc
     6661 gcctagcatt tcatcacatg gcccgagagc tgcatccgga ctgtactggg tctctctggt
     6721 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     6781 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     6841 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagc atgtgagcaa
     6901 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     6961 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     7021 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     7081 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     7141 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     7201 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     7261 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     7321 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     7381 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     7441 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     7501 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     7561 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     7621 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     7681 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     7741 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     7801 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     7861 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     7921 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     7981 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     8041 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     8101 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     8161 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     8221 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     8281 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     8341 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     8401 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     8461 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     8521 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     8581 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     8641 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     8701 acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc
     8761 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
     8821 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
     8881 tctgcttagg gttaggcgtt ttgcgctgct tcgc
//
