LOCUS       sequence-211897-        8914 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FANCF_-_22647359.23-P1P2 sgRNA in pCR1068.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8914)
  AUTHORS   Richardson CD, Kazane KR, Feng SJ, Zelin E, Bray NL, Schafer AJ, 
            Floor SN, Corn JE
  TITLE     CRISPR-Cas9 genome editing in human cells occurs via the Fanconi 
            anemia pathway.
  JOURNAL   Nat Genet. 2018 Aug;50(8):1132-1139. doi: 10.1038/s41588-018-0174-0.
            Epub 2018 Jul 27.
  PUBMED    30054595
REFERENCE   2  (bases 1 to 8914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41588-018-0174-0"; journalName: "Nat Genet"; date: 
            "2018-08"; volume: "50"; issue: "8"; pages: "1132-1139"
COMMENT     Sequence Label: pCR1224
FEATURES             Location/Qualifiers
     source          1..8914
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        16..395
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        397..595
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     550..570
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             613..793
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    840..965
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1462..1695
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1880..1924
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2222..2339
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(2600..2633)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     2637..2657
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     promoter        2910..3121
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          3122..4060
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4017..4037
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4081..4677
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4081..4100)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4577..4597
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4687..4740
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      4747..4756
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4753..4755,4756..4758,4759..5472)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4798..4819)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5059..5078)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5406..5427
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(5492..5525)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    5581..6169
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5634..5654)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6052..6063)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6172..6188)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6173..6189)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6698..6878
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(6940..7528)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7020..7039)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7699..8490,8491..8559))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8322..8341
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8560..8664)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8739..8758)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca attacggggt
       61 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
      121 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
      181 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
      241 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
      301 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
      361 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
      421 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
      481 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
      541 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagcgcgt
      601 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
      661 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
      721 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
      781 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
      841 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
      901 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
      961 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
     1021 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
     1081 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
     1141 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
     1201 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     1261 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccggc cgctgatctt
     1321 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     1381 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     1441 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     1501 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     1561 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     1621 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     1681 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     1741 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     1801 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     1861 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     1921 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     1981 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     2041 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga
     2101 caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt
     2161 agtgaacgga tcggcactgc gtgcgccaat tctgcagaca aatggcagta ttcatccaca
     2221 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     2281 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
     2341 gggtttatta cagggacagc agagatccag tttggttagt accgggcccg ccctgcaggt
     2401 ctagagatcc gacgcgccat ctctaggccc gcgccggccc cctcgcacgg acttgtggga
     2461 gaagctcggc tactcccctg ccccggttaa tttgcatata atatttccta gtaactatag
     2521 aggcttaatg tgcgataaaa gacagataat ctgttctttt taatactagc tacattttac
     2581 atgataggct tggatttcta taacttcgta tagcatacat tatacgaagt tataaacagc
     2641 acaaaaggaa actcacccta actgtaaagt aattgtgtgt tttgagacta taagtatccc
     2701 ttggagaacc accttgttgg ctgcagaagg gattccatgg tttaagagct aagctggaaa
     2761 cagcatagca agtttaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc
     2821 ggtgcttttt ttctcgagta ctaggatcca ttaggcggcc gccctgcagg gtggataacc
     2881 gtattaccgc catgcatgtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga
     2941 gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa
     3001 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta
     3061 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca
     3121 ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt
     3181 gccttgaatt acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg
     3241 aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt
     3301 tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg
     3361 tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct
     3421 ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt
     3481 ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg
     3541 gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc
     3601 tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg
     3661 gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc
     3721 aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag
     3781 ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag
     3841 gcacctcgat tagttctcgc gcttttggag tacgtcgtct ttaggttggg gggaggggtt
     3901 ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca
     3961 cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa
     4021 gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgag ctagcccacc
     4081 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta
     4141 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgacccggac
     4201 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     4261 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     4321 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     4381 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     4441 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     4501 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     4561 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     4621 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgccgga
     4681 tcgggagagg gcagaggaag tctgctaaca tgcggtgacg tcgaggagaa tcctggccca
     4741 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     4801 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     4861 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgccggtg
     4921 ccctggccca ccctcgtgac caccctgacc catggcgtgc agtgcttcag ccgctacccc
     4981 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     5041 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     5101 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     5161 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     5221 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     5281 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     5341 cccgacaacc actacctgag cacccagtcc aagctgagta aagaccccaa cgagaagcgc
     5401 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     5461 ctgtacaagt aagaattcgt cgagggacct aataacttcg tatagcatac attatacgaa
     5521 gttatacatg tttaagggtt ccggttccac taggtacaat tcgatatcaa gcttatcgat
     5581 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     5641 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     5701 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     5761 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     5821 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     5881 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     5941 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     6001 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     6061 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     6121 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt
     6181 cgacctcgat cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac
     6241 caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac
     6301 acctcaggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     6361 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga
     6421 tctgtggatc taccacacac aaggctactt ccctgattgg cagaactaca caccagggcc
     6481 agggatcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagcaaga
     6541 gaaggtagaa gaagccaatg aaggagagaa cacccgcttg ttacaccctg tgagcctgca
     6601 tgggatggat gacccggaga gagaagtatt agagtggagg tttgacagcc gcctagcatt
     6661 tcatcacatg gcccgagagc tgcatccgga ctgtactggg tctctctggt tagaccagat
     6721 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     6781 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     6841 cctcagaccc ttttagtcag tgtggaaaat ctctagcagc atgtgagcaa aaggccagca
     6901 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     6961 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     7021 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     7081 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     7141 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     7201 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     7261 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     7321 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     7381 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     7441 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     7501 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     7561 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     7621 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     7681 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     7741 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     7801 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     7861 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     7921 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     7981 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     8041 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     8101 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     8161 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     8221 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     8281 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     8341 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     8401 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     8461 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     8521 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     8581 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     8641 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
     8701 atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca
     8761 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc
     8821 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
     8881 gttaggcgtt ttgcgctgct tcgcgatgta cggg
//
