LOCUS       sequence-211899-        8914 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FANCI_+_89787428.23-P1P2 sgRNA in pCR1068.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8914)
  AUTHORS   Richardson CD, Kazane KR, Feng SJ, Zelin E, Bray NL, Schafer AJ, 
            Floor SN, Corn JE
  TITLE     CRISPR-Cas9 genome editing in human cells occurs via the Fanconi 
            anemia pathway.
  JOURNAL   Nat Genet. 2018 Aug;50(8):1132-1139. doi: 10.1038/s41588-018-0174-0.
            Epub 2018 Jul 27.
  PUBMED    30054595
REFERENCE   2  (bases 1 to 8914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41588-018-0174-0"; journalName: "Nat Genet"; date: 
            "2018-08"; volume: "50"; issue: "8"; pages: "1132-1139"
COMMENT     Sequence Label: pCR1272
FEATURES             Location/Qualifiers
     source          1..8914
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        21..400
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        402..600
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     555..575
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             618..798
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    845..970
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1467..1700
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1885..1929
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2227..2344
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(2605..2638)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     2642..2662
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     promoter        2915..3126
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          3127..4065
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4022..4042
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4086..4682
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4086..4105)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4582..4602
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4692..4745
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      4752..4761
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4758..4760,4761..4763,4764..5477)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4803..4824)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5064..5083)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5411..5432
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(5497..5530)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    5586..6174
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5639..5659)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6057..6068)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6177..6193)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6178..6194)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6703..6883
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(6945..7533)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7025..7044)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7704..8495,8496..8564))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8327..8346
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8565..8669)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8744..8763)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac
       61 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
      121 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
      181 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
      241 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
      301 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
      361 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
      421 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
      481 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
      541 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
      601 cgcgttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
      661 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
      721 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
      781 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
      841 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
      901 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
      961 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
     1021 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
     1081 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
     1141 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
     1201 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
     1261 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg
     1321 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
     1381 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
     1441 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
     1501 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
     1561 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     1621 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
     1681 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
     1741 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
     1801 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
     1861 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
     1921 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
     1981 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
     2041 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
     2101 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
     2161 cgattagtga acggatcggc actgcgtgcg ccaattctgc agacaaatgg cagtattcat
     2221 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     2281 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     2341 ttttcgggtt tattacaggg acagcagaga tccagtttgg ttagtaccgg gcccgccctg
     2401 caggtctaga gatccgacgc gccatctcta ggcccgcgcc ggccccctcg cacggacttg
     2461 tgggagaagc tcggctactc ccctgccccg gttaatttgc atataatatt tcctagtaac
     2521 tatagaggct taatgtgcga taaaagacag ataatctgtt ctttttaata ctagctacat
     2581 tttacatgat aggcttggat ttctataact tcgtatagca tacattatac gaagttataa
     2641 acagcacaaa aggaaactca ccctaactgt aaagtaattg tgtgttttga gactataagt
     2701 atcccttgga gaaccacctt gttgggaacc gcgaaagctg aggcgtttaa gagctaagct
     2761 ggaaacagca tagcaagttt aaataaggct agtccgttat caacttgaaa aagtggcacc
     2821 gagtcggtgc tttttttctc gagtactagg atccattagg cggccgccct gcagggtgga
     2881 taaccgtatt accgccatgc atgtgcccgt cagtgggcag agcgcacatc gcccacagtc
     2941 cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg
     3001 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
     3061 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
     3121 acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct
     3181 tgcgtgcctt gaattacttc cacctggctg cagtacgtga ttcttgatcc cgagcttcgg
     3241 gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct
     3301 tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc
     3361 gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg
     3421 acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt
     3481 tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg
     3541 aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg
     3601 cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg
     3661 gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg
     3721 agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg
     3781 aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg
     3841 tccaggcacc tcgattagtt ctcgcgcttt tggagtacgt cgtctttagg ttggggggag
     3901 gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct
     3961 tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt
     4021 ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgagctagc
     4081 ccaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg
     4141 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc
     4201 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     4261 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
     4321 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     4381 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     4441 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
     4501 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     4561 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     4621 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
     4681 ccggatcggg agagggcaga ggaagtctgc taacatgcgg tgacgtcgag gagaatcctg
     4741 gcccaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     4801 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     4861 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     4921 cggtgccctg gcccaccctc gtgaccaccc tgacccatgg cgtgcagtgc ttcagccgct
     4981 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     5041 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     5101 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     5161 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     5221 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     5281 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     5341 tgctgcccga caaccactac ctgagcaccc agtccaagct gagtaaagac cccaacgaga
     5401 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     5461 acgagctgta caagtaagaa ttcgtcgagg gacctaataa cttcgtatag catacattat
     5521 acgaagttat acatgtttaa gggttccggt tccactaggt acaattcgat atcaagctta
     5581 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     5641 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     5701 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     5761 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     5821 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     5881 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     5941 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     6001 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     6061 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     6121 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat
     6181 accgtcgacc tcgatcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca
     6241 gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca
     6301 gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     6361 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatatc
     6421 cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa ctacacacca
     6481 gggccaggga tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag
     6541 caagagaagg tagaagaagc caatgaagga gagaacaccc gcttgttaca ccctgtgagc
     6601 ctgcatggga tggatgaccc ggagagagaa gtattagagt ggaggtttga cagccgccta
     6661 gcatttcatc acatggcccg agagctgcat ccggactgta ctgggtctct ctggttagac
     6721 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     6781 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     6841 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagcatgtg agcaaaaggc
     6901 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     6961 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     7021 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     7081 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     7141 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     7201 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     7261 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     7321 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     7381 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     7441 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     7501 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     7561 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     7621 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     7681 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     7741 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     7801 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     7861 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     7921 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     7981 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     8041 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     8101 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     8161 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     8221 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     8281 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     8341 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     8401 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     8461 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     8521 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     8581 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     8641 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     8701 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
     8761 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
     8821 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
     8881 ttagggttag gcgttttgcg ctgcttcgcg atgt
//
