LOCUS       sequence-21190-e         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Kurosu T, Zhang F, Peterlin BM
  TITLE     Transcriptional activity and substrate recognition of cyclin T2 from
            P-TEFb.
  JOURNAL   Gene. 2004 Dec 8. 343(1):173-9.
  PUBMED    15563843
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene. 2004 
            Dec 8. 343(1):173-9."
COMMENT     Sequence Label: pEF myc Gal cycT2b (1-420) (P#1720)
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             88..105
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             115..144
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             160..600
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
ORIGIN
        1 ctctttcggt gtcgtgagga ttagcttgct tacatttgct tctgacacaa ctgtgttcac
       61 tagcaacctc aaacagacac catgccgcat caccaccatc atcacgccat gccggagcag
      121 aagctgatat ccgaggagga cctggccatg gccggatcca tgaagctact gtcttctatc
      181 gaacaagcat gcgatatttg ccgacttaaa aagctcaagt gctccaaaga aaaaccgaag
      241 tgcgccaagt gtctgaagaa caactgggag tgtcgctact ctcccaaaac caaaaggtct
      301 ccgctgacta gggcacatct gacagaagtg gaatcaaggc tagaaagact ggaacagcta
      361 tttctactga tttttcctcg agaagacctt gacatgattt tgaaaatgga ttctttacag
      421 gatataaaag cattgttaac aggattattt gtacaagata atgtgaataa agatgccgtc
      481 acagatagat tggcttcagt ggagactgat atgcctctaa cattgagaca gcatagaata
      541 agtgcgacat catcatcgga agagagtagt aacaaaggtc aaagacagtt gactgtatcg
      601 ccggaattcg tcgacatggc gtcgggccgt ggagcttctt ctcgctggtt ctttactcgg
      661 gaacagctgg agaacacgcc gagccgccgc tgcggagtgg aggcggataa agagctctcg
      721 tgccgtggcg gnngnaggng ncancctcat cnggaanggg nagcnnncng tncncccnat
      781 taaaaaacct gnaatgttgn ngccaggttt ntnncccntn ntccnaattc anaaanantt
      841 ngtcnng
//
