LOCUS       sequence-211900-        8063 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human WT KCNB1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8063)
  AUTHORS   Cotella D, Hernandez-Enriquez B, Wu X, Li R, Pan Z, Leveille J, Link
            CD, Oddo S, Sesti F
  TITLE     Toxic role of K+ channel oxidation in mammalian brain.
  JOURNAL   J Neurosci. 2012 Mar 21;32(12):4133-44. doi: 
            10.1523/JNEUROSCI.6153-11.2012.
  PUBMED    22442077
REFERENCE   2  (bases 1 to 8063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.6153-11"; journalName: "J Neurosci"; date: 
            "2012-03-21- 21"; volume: "32"; issue: "12"; pages: "4133-44"
COMMENT     Sequence Label: hKv2.1-HA
FEATURES             Location/Qualifiers
     source          1..8063
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     80..100
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     126..150
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          290..422
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     405..424
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        467..485
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3091..3117
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    3167..3288
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3178..3197)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3232..3251
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      3474..3929
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3561..3580)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3771..3792
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4046..4403
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4046..4237
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4069..4089)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4254..4389
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4454..5248
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4508..4527)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5118..5137
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5312..5360
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(5401..5420)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5520..5542
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5580..5598)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5666..5770
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5771..5839,5840..6631)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5989..6008)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6802..7390
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7291..7310
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        7601..7980
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        join(7981..8063,1..129)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
       61 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      121 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      181 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      241 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      301 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      361 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
      421 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
      481 ataggctagc ctcgagagct gaattccacc atgccggcgg gcatgacgaa gcatggctcc
      541 cgctccacca gctcgctgcc gcccgagccc atggagatcg tgcgcagcaa ggcgtgctct
      601 cggcgggtcc gcctcaacgt cggggggctg gcgcacgagg tactctggcg taccctggac
      661 cgcctgcccc gcacgcggct gggcaagctc cgcgactgca acacgcacga ctcgctgctg
      721 gaggtgtgcg atgactacag cctcgacgac aacgagtact tctttgaccg ccacccgggc
      781 gccttcacct ccatcctcaa cttctaccgc actgggcgac tgcacatgat ggaggagatg
      841 tgcgcgctca gcttcagcca agagctcgac tactggggca tcgacgagat ctacccggag
      901 tcctgctgcc aggcccgcta ccaccagaag aaagagcaga tgaacgagga gctcaagcgt
      961 gaggccgaga ccctacggga gcgggaaggc gaggagttcg ataacacgtg ctgcgcagag
     1021 aagaggaaaa aactctggga cctactggag aagcccaatt cctctgtggc tgccaagatc
     1081 cttgccataa tttccatcat gttcatcgtc ctctccacca ttgccctgtc cctcaacacg
     1141 ctgcctgagc tacagagcct cgatgagttc ggccagtcca cagacaaccc ccagctggcc
     1201 cacgtggagg ccgtgtgcat cgcatggttc accatggagt acctgctgag gttcctctcc
     1261 tcgcccaaga agtggaagtt cttcaagggc ccactcaatg ccattgactt gttggccatt
     1321 ctgccatact atgtcaccat tttcctcacc gaatccaaca agagcgtgct gcaattccag
     1381 aatgtccgcc gcgtggtcca gatcttccgc atcatgcgca ttctccgcat ccttaagctt
     1441 gcacgccact ccactggcct ccagtctctg ggcttcactt tgcggaggag ctacaatgag
     1501 ttgggcttgc tcatcctctt ccttgccatg ggcattatga tcttctccag ccttgtcttc
     1561 tttgctgaga aggatgagga cgacaccaag ttcaaaagca tcccagcctc tttctggtgg
     1621 gccaccatca ccatgactac tgttgggtat ggagacatct accccaagac tctcctgggg
     1681 aaaattgttg ggggactctg ctgcattgca ggagtcctgg tgattgctct tcccatcccc
     1741 atcatcgtca ataacttctc tgagttctat aaggagcaga agagacagga gaaagcaatc
     1801 aaacggcgag aggctctgga gagagccaag aggaatggca gcatcgtatc catgaacatg
     1861 aaggatgctt ttgcccggag cattgagatg atggacattg tggttgagaa aaatggggag
     1921 aatatgggta agaaagacaa agtacaagat aaccacttgt ctcctaacaa atggaaatgg
     1981 acaaagagga cactgtctga aaccagctca agtaagtcct ttgaaaccaa ggaacaggga
     2041 tcccctgaaa aagccagatc gtcttctagt cctcagcacc tgaacgttca gcagttggaa
     2101 gacatgtaca ataagatggc caagacccaa tcccaaccca tcctcaatac caaggagtca
     2161 gcagcacaga gcaaaccaaa ggaagaactt gaaatggaga gtatccccag ccccgtagcc
     2221 cctctgccca ctcgcacaga aggggtcatt gacatgcgaa gtatgtcaag cattgatagt
     2281 ttcattagct gtgccacaga cttccctgag gccaccagat tctcccacag ccctttgaca
     2341 tcactcccca gcaagactgg gggcagcaca gccccagaag tgggctggcg gggagctctg
     2401 ggtgccagtg gtggtaggtt tgtggaggcc aaccccagcc ctgatgccag ccagcactct
     2461 agtttcttca tcgagagccc caagagttcc atgaaaacta acaacccttt gaagctccga
     2521 gcacttaaag tcaacttcat ggagggtgac cccagtccac tcctccccgt tctagggatg
     2581 taccatgacc ctctcaggaa ccgggggagt gctgcggctg ctgtcgctgg actggagtgt
     2641 gccacgcttt tggacaaggc tgtgctgagc ccagagtcct ccatctacac cacagcaagt
     2701 gctaagacac ccccccggtc tcctgagaaa cacacagcaa tagcgttcaa ctttgaggcg
     2761 ggtgtccacc agtacattga cgcagacaca gatgatgagg gacagctgct ctacagtgtg
     2821 gactccagcc cccccaaaag cctccctggg agcaccagtc cgaagttcag cacggggaca
     2881 agatcggaga aaaaccactt tgaaagctcc cctttaccca cctcccctaa gttcttaagg
     2941 cagaactgta tttactccac agaagcattg actggaaaag gccccagtgg tcaggaaaag
     3001 tgcaaacttg agaaccacat ctcccctgac gtccgtgtgt tgccaggggg aggagcccat
     3061 ggaagcacac gagatcagag catcgaattc tacccatacg atgttccaga ttacgcttag
     3121 gcggccgctt ccctttagtg agggttaatg cttcgagcag acatgataag atacattgat
     3181 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     3241 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     3301 tgcattcatt ttatgtttca ggttcagggg gagatgtggg aggtttttta aagcaagtaa
     3361 aacctctaca aatgtggtaa aatccgataa ggatcgatcc gggctggcgt aatagcgaag
     3421 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggacgcgcc
     3481 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     3541 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     3601 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     3661 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     3721 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     3781 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     3841 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     3901 ttttaacaaa atattaacgc ttacaatttc ctgatgcggt attttctcct tacgcatctg
     3961 tgcggtattt cacaccgcat acgcggatct gcgcagcacc atggcctgaa ataacctctg
     4021 aaagaggaac ttggttaggt accttctgag gcggaaagaa ccagctgtgg aatgtgtgtc
     4081 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     4141 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     4201 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     4261 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     4321 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     4381 ttggaggcct aggcttttgc aaaaagcttg attcttctga cacaacagtc tcgaacttaa
     4441 ggctagagcc accatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     4501 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     4561 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     4621 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     4681 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     4741 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     4801 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     4861 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     4921 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     4981 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     5041 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     5101 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     5161 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     5221 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
     5281 gcgacgccca acctgccatc acgatggccg caataaaata tctttatttt cattacatct
     5341 gtgtgttggt tttttgtgtg aatcgatagc gataaggatc cgcgtatggt gcactctcag
     5401 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     5461 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     5521 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     5581 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     5641 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     5701 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     5761 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     5821 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     5881 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     5941 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     6001 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     6061 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     6121 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     6181 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     6241 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     6301 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     6361 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     6421 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     6481 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     6541 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     6601 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     6661 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     6721 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     6781 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     6841 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     6901 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     6961 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     7021 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     7081 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     7141 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     7201 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     7261 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     7321 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     7381 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     7441 tgctcacatg gctcgacaga tcttcaatat tggccattag ccatattatt cattggttat
     7501 atagcataaa tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg
     7561 tacatttata ttggctcatg tccaatatga ccgccatgtt ggcattgatt attgactagt
     7621 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     7681 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     7741 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     7801 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     7861 ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     7921 accttacggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     7981 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
     8041 ccaagtctcc accccattga cgt
//
