LOCUS       sequence-211906-        8937 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses HELQ_-_84376979.23-P1P2 sgRNA in pCR1068.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8937)
  AUTHORS   Richardson CD, Kazane KR, Feng SJ, Zelin E, Bray NL, Schafer AJ, 
            Floor SN, Corn JE
  TITLE     CRISPR-Cas9 genome editing in human cells occurs via the Fanconi 
            anemia pathway.
  JOURNAL   Nat Genet. 2018 Aug;50(8):1132-1139. doi: 10.1038/s41588-018-0174-0.
            Epub 2018 Jul 27.
  PUBMED    30054595
REFERENCE   2  (bases 1 to 8937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41588-018-0174-0"; journalName: "Nat Genet"; date: 
            "2018-08"; volume: "50"; issue: "8"; pages: "1132-1139"
COMMENT     Sequence Label: pCR1165
FEATURES             Location/Qualifiers
     source          1..8937
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        41..420
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        422..620
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     575..595
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             638..818
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    865..990
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1487..1720
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1905..1949
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2247..2364
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(2625..2658)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     2662..2682
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     promoter        2958..3169
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          3170..4108
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4065..4085
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4129..4725
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4129..4148)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4625..4645
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4735..4788
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      4795..4804
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4801..4803,4804..4806,4807..5520)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4846..4867)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5107..5126)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5454..5475
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(5540..5573)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    5629..6217
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5682..5702)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6100..6111)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6220..6236)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6221..6237)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6746..6926
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(6988..7576)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7068..7087)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7747..8538,8539..8607))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8370..8389
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8608..8712)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8787..8806)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt
       61 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
      121 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
      181 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
      241 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
      301 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
      361 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
      421 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
      481 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      541 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      601 tgggaggtct atataagcag cgcgttttgc ctgtactggg tctctctggt tagaccagat
      661 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
      721 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
      781 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
      841 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
      901 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
      961 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     1021 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     1081 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     1141 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     1201 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     1261 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     1321 cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     1381 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     1441 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     1501 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     1561 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     1621 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     1681 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     1741 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     1801 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     1861 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     1921 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     1981 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     2041 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     2101 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     2161 gagacagaga cagatccatt cgattagtga acggatcggc actgcgtgcg ccaattctgc
     2221 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
     2281 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     2341 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg
     2401 ttagtaccgg gcccgccctg caggtctaga gatccgacgc gccatctcta ggcccgcgcc
     2461 ggccccctcg cacggacttg tgggagaagc tcggctactc ccctgccccg gttaatttgc
     2521 atataatatt tcctagtaac tatagaggct taatgtgcga taaaagacag ataatctgtt
     2581 ctttttaata ctagctacat tttacatgat aggcttggat ttctataact tcgtatagca
     2641 tacattatac gaagttataa acagcacaaa aggaaactca ccctaactgt aaagtaattg
     2701 tgtgttttga gactataagt atcccttgga gaaccacctt gttgggaccc caagttagct
     2761 ctcagtttaa gagctagtta gctctcagtt taagagctaa gctggaaaca gcatagcaag
     2821 tttaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttt
     2881 ctcgagtact aggatccatt aggcggccgc cctgcagggt ggataaccgt attaccgcca
     2941 tgcatgtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
     3001 aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
     3061 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
     3121 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt
     3181 gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc cttgaattac
     3241 ttccacctgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa gtgggtggga
     3301 gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc
     3361 tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt
     3421 cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca
     3481 agatagtctt gtaaatgcgg gccaagatct gcacactggt atttcggttt ttggggccgc
     3541 gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc
     3601 gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg
     3661 cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt
     3721 tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa aatggaggac
     3781 gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg cctttccgtc
     3841 ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta
     3901 gttctcgcgc ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga
     3961 gtttccccac actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt
     4021 ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt
     4081 ggttcaaagt ttttttcttc catttcaggt gtcgtgagct agcccaccat gaccgagtac
     4141 aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg caccctcgcc
     4201 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg acccggaccg ccacatcgag
     4261 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     4321 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     4381 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     4441 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     4501 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     4561 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     4621 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     4681 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgccggatc gggagagggc
     4741 agaggaagtc tgctaacatg cggtgacgtc gaggagaatc ctggcccacc ggtcgccacc
     4801 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     4861 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     4921 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgccggtgcc ctggcccacc
     4981 ctcgtgacca ccctgaccca tggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     5041 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     5101 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     5161 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     5221 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     5281 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     5341 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     5401 tacctgagca cccagtccaa gctgagtaaa gaccccaacg agaagcgcga tcacatggtc
     5461 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
     5521 gaattcgtcg agggacctaa taacttcgta tagcatacat tatacgaagt tatacatgtt
     5581 taagggttcc ggttccacta ggtacaattc gatatcaagc ttatcgataa tcaacctctg
     5641 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     5701 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     5761 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     5821 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     5881 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     5941 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     6001 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     6061 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     6121 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     6181 cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgatcg
     6241 agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg
     6301 tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc
     6361 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     6421 gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta
     6481 ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag ggatcagata
     6541 tccactgacc tttggatggt gctacaagct agtaccagtt gagcaagaga aggtagaaga
     6601 agccaatgaa ggagagaaca cccgcttgtt acaccctgtg agcctgcatg ggatggatga
     6661 cccggagaga gaagtattag agtggaggtt tgacagccgc ctagcatttc atcacatggc
     6721 ccgagagctg catccggact gtactgggtc tctctggtta gaccagatct gagcctggga
     6781 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     6841 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     6901 ttagtcagtg tggaaaatct ctagcagcat gtgagcaaaa ggccagcaaa aggccaggaa
     6961 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     7021 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     7081 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     7141 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     7201 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     7261 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     7321 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     7381 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     7441 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     7501 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     7561 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     7621 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     7681 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     7741 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     7801 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     7861 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     7921 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     7981 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     8041 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     8101 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     8161 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     8221 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     8281 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     8341 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     8401 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     8461 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     8521 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     8581 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     8641 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     8701 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct
     8761 cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     8821 tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct
     8881 acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgt
//
