LOCUS       sequence-211910-        8914 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses RAD51C_+_56770199.23-P1P2 sgRNA in pCR1068.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8914)
  AUTHORS   Richardson CD, Kazane KR, Feng SJ, Zelin E, Bray NL, Schafer AJ, 
            Floor SN, Corn JE
  TITLE     CRISPR-Cas9 genome editing in human cells occurs via the Fanconi 
            anemia pathway.
  JOURNAL   Nat Genet. 2018 Aug;50(8):1132-1139. doi: 10.1038/s41588-018-0174-0.
            Epub 2018 Jul 27.
  PUBMED    30054595
REFERENCE   2  (bases 1 to 8914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41588-018-0174-0"; journalName: "Nat Genet"; date: 
            "2018-08"; volume: "50"; issue: "8"; pages: "1132-1139"
COMMENT     Sequence Label: pCR1275
FEATURES             Location/Qualifiers
     source          1..8914
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        19..398
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        400..598
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     553..573
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             616..796
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    843..968
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1465..1698
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1883..1927
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2225..2342
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(2603..2636)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     2640..2660
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     promoter        2913..3124
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          3125..4063
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4020..4040
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4084..4680
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4084..4103)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4580..4600
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4690..4743
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      4750..4759
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4756..4758,4759..4761,4762..5475)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4801..4822)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5062..5081)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5409..5430
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(5495..5528)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    5584..6172
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5637..5657)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6055..6066)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6175..6191)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6176..6192)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6701..6881
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(6943..7531)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7023..7042)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7702..8493,8494..8562))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8325..8344
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8563..8667)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8742..8761)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
       61 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
      121 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      181 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      241 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
      301 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
      361 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
      421 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
      481 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
      541 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagcg
      601 cgttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
      661 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
      721 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
      781 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
      841 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
      901 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
      961 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
     1021 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
     1081 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     1141 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     1201 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     1261 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat
     1321 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     1381 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     1441 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     1501 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     1561 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     1621 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     1681 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     1741 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     1801 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     1861 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     1921 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     1981 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     2041 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     2101 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
     2161 attagtgaac ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc
     2221 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     2281 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     2341 ttcgggttta ttacagggac agcagagatc cagtttggtt agtaccgggc ccgccctgca
     2401 ggtctagaga tccgacgcgc catctctagg cccgcgccgg ccccctcgca cggacttgtg
     2461 ggagaagctc ggctactccc ctgccccggt taatttgcat ataatatttc ctagtaacta
     2521 tagaggctta atgtgcgata aaagacagat aatctgttct ttttaatact agctacattt
     2581 tacatgatag gcttggattt ctataacttc gtatagcata cattatacga agttataaac
     2641 agcacaaaag gaaactcacc ctaactgtaa agtaattgtg tgttttgaga ctataagtat
     2701 cccttggaga accaccttgt tggggcgaga gaacgaagac tggtttaaga gctaagctgg
     2761 aaacagcata gcaagtttaa ataaggctag tccgttatca acttgaaaaa gtggcaccga
     2821 gtcggtgctt tttttctcga gtactaggat ccattaggcg gccgccctgc agggtggata
     2881 accgtattac cgccatgcat gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc
     2941 cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt
     3001 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc
     3061 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac
     3121 acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg
     3181 cgtgccttga attacttcca cctggctgca gtacgtgatt cttgatcccg agcttcgggt
     3241 tggaagtggg tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg
     3301 agttgaggcc tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc
     3361 ctgtctcgct gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac
     3421 gctttttttc tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc
     3481 ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag
     3541 gcggggcctg cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc
     3601 tgctctggtg cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc
     3661 ccggtcggca ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag
     3721 ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa
     3781 aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc
     3841 caggcacctc gattagttct cgcgcttttg gagtacgtcg tctttaggtt ggggggaggg
     3901 gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg
     3961 gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct
     4021 caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt gagctagccc
     4081 accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc
     4141 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgacccg
     4201 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
     4261 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
     4321 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
     4381 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
     4441 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
     4501 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
     4561 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
     4621 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
     4681 ggatcgggag agggcagagg aagtctgcta acatgcggtg acgtcgagga gaatcctggc
     4741 ccaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     4801 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     4861 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccg
     4921 gtgccctggc ccaccctcgt gaccaccctg acccatggcg tgcagtgctt cagccgctac
     4981 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     5041 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     5101 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     5161 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     5221 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     5281 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     5341 ctgcccgaca accactacct gagcacccag tccaagctga gtaaagaccc caacgagaag
     5401 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     5461 gagctgtaca agtaagaatt cgtcgaggga cctaataact tcgtatagca tacattatac
     5521 gaagttatac atgtttaagg gttccggttc cactaggtac aattcgatat caagcttatc
     5581 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     5641 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     5701 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     5761 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     5821 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     5881 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     5941 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     6001 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     6061 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     6121 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac
     6181 cgtcgacctc gatcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc
     6241 taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt
     6301 cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     6361 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatatcct
     6421 tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact acacaccagg
     6481 gccagggatc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagca
     6541 agagaaggta gaagaagcca atgaaggaga gaacacccgc ttgttacacc ctgtgagcct
     6601 gcatgggatg gatgacccgg agagagaagt attagagtgg aggtttgaca gccgcctagc
     6661 atttcatcac atggcccgag agctgcatcc ggactgtact gggtctctct ggttagacca
     6721 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     6781 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     6841 atccctcaga cccttttagt cagtgtggaa aatctctagc agcatgtgag caaaaggcca
     6901 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     6961 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     7021 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     7081 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     7141 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     7201 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     7261 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     7321 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     7381 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     7441 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     7501 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     7561 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     7621 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     7681 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     7741 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     7801 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     7861 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     7921 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     7981 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     8041 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     8101 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     8161 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     8221 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     8281 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     8341 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     8401 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     8461 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     8521 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     8581 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     8641 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga
     8701 cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc
     8761 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
     8821 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
     8881 agggttaggc gttttgcgct gcttcgcgat gtac
//
