LOCUS       sequence-211911-       10874 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fast anion channelrhodopsin (current half-decay time 3-10 ms 
            depending on voltage and light intensity) for time-resolved 
            inhibition of neuronal spiking.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10874)
  AUTHORS   Govorunova EG, Sineshchekov OA, Hemmati R, Janz R, Morelle O, 
            Melkonian M, Wong GK, Spudich JL
  TITLE     Extending the Time Domain of Neuronal Silencing with Cryptophyte 
            Anion Channelrhodopsins.
  JOURNAL   eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-0174-18. doi: 
            10.1523/ENEURO.0174-18.2018. eCollection 2018 May-Jun.
  PUBMED    30027111
REFERENCE   2  (bases 1 to 10874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eNeuro."; 
            date: "2018-07-10"; volume: "5(3). pii: eN-MNT-0174-18. doi"; pages:
            " 10.1523/ENEURO.0174-18.2018. eCollection 2018 May-Jun"
COMMENT     Sequence Label: pFUGW-RapACR-EYFP
FEATURES             Location/Qualifiers
     source          1..10874
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        79..1290
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1127..1146
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(2255..2257,2258..2971)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(2297..2318)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2558..2577)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2905..2926
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2996..3584
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3049..3069)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3467..3478)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3587..3603)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3588..3604)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4109..4289
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4315..4332)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4321..4545
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4591..5019
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4678..4697)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4888..4909
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5028..5048)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5033..5362
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5213..5348
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5275..5294
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5410..5457
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5476..5850
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5980..6101
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6017..6036)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6071..6090
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6150..6166)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6150..6166)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6163..6185)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6174..6190
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6198..6204,6205..6222,6223..6228))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6243..6264
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6381..6398)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6552..7140)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6632..6651)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7311..8102,8103..8171))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7934..7953
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8172..8276)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8351..8370)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8542..8921
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8923..9121
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9076..9096
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9139..9319
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9366..9491
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9984..10217
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10402..10446
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10744..10861
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gggacagcag agatccagtt tggttaatcg catgctagct agttcgaacc ttaattaacc
       61 cgtgtcggct ccagatctgg cctccgcgcc gggttttggc gcctcccgcg ggcgcccccc
      121 tcctcacggc gagcgctgcc acgtcagacg aagggcgcag cgagcgtcct gatccttccg
      181 cccggacgct caggacagcg gcccgctgct cataagactc ggccttagaa ccccagtatc
      241 agcagaagga cattttagga cgggacttgg gtgactctag ggcactggtt ttctttccag
      301 agagcggaac aggcgaggaa aagtagtccc ttctcggcga ttctgcggag ggatctccgt
      361 ggggcggtga acgccgatga ttatataagg acgcgccggg tgtggcacag ctagttccgt
      421 cgcagccggg atttgggtcg cggttcttgt ttgtggatcg ctgtgatcgt cacttggtga
      481 gtagcgggct gctgggctgg ccggggcttt cgtggccgcc gggccgctcg gtgggacgga
      541 agcgtgtgga gagaccgcca agggctgtag tctgggtccg cgagcaaggt tgccctgaac
      601 tgggggttgg ggggagcgca gcaaaatggc ggctgttccc gagtcttgaa tggaagacgc
      661 ttgtgaggcg ggctgtgagg tcgttgaaac aaggtggggg gcatggtggg cggcaagaac
      721 ccaaggtctt gaggccttcg ctaatgcggg aaagctctta ttcgggtgag atgggctggg
      781 gcaccatctg gggaccctga cgtgaagttt gtcactgact ggagaactcg gtttgtcgtc
      841 tgttgcgggg gcggcagtta tggcggtgcc gttgggcagt gcacccgtac ctttgggagc
      901 gcgcgccctc gtcgtgtcgt gacgtcaccc gttctgttgg cttataatgc agggtggggc
      961 cacctgccgg taggtgtgcg gtaggctttt ctccgtcgca ggacgcaggg ttcgggccta
     1021 gggtaggctc tcctgaatcg acaggcgccg gacctctggt gaggggaggg ataagtgagg
     1081 cgtcagtttc tttggtcggt tttatgtacc tatcttctta agtagctgaa gctccggttt
     1141 tgaactatgc gctcggggtt ggcgagtgtg ttttgtgaag ttttttaggc accttttgaa
     1201 atgtaatcat ttgggtcaat atgtaatttt cagtgttaga ctagtaaatt gtccgctaaa
     1261 ttctggccgt ttttggcttt tttgttagac gaagcttggg ctgcaggtcg actctagagg
     1321 atccatggac tacaccagcc tggacgattt tccactggag acactgtact tccggaacgg
     1381 cacagaggtg gacccctatg atcacagatg caagctgggc atccctggag gctcccaggg
     1441 catccacaca tttcagctgg tgtgggcact gctgtgcgca gcacaggccc tgtttctgtt
     1501 ctccaagtgg ccaaaagtgg gctgggaggc catgtacatg ccctgctgtg aggccctgtc
     1561 ttatggcctg ctgagctccg gctacggcta tatgaagctg cacggcgagt tccactacct
     1621 gccttggatc aggtacgccg cctggctggt gaccacacca gtgctgctga accaggtgca
     1681 gggcatgatc gacatcaaga tcggctctct gaggctggca ccaatccaga gcctggcaaa
     1741 cctgatcatg atcatcatgg gcgtgagcgc ccacctgcag gagaatcacg cctggaagtg
     1801 gctgttcttt ttcgtgggct gcgtgatgtg cgccgtgatc tactatatcg cctggctgca
     1861 gtttgccaaa gtgaatgagc tgtacgccgc caccaagaca gagatcggcg ataaggtgtg
     1921 ctttcagctg aagctgatca tgttttgttt ctatgtgggc tggtccatgt tctctgtgat
     1981 ctggctgctg tctagcgagg gcctgtgcct gatcgaggag cacatcagcg ccgccgccca
     2041 catcccaggc gacatcctga gcaagaactc ctttggcctg ctgatgtggt ataccaggtg
     2101 gatcacactg ggaggagcat gggatatcaa taagttcctg gagaagcagg ccaaggagaa
     2161 ggcagaggcc gtgctgaccc ctgccgagaa gaggaaggcc atcatgaagc aggccgaggc
     2221 ctgcttctcc atggccgatg cctgtgcggc cgccgtgagc aagggcgagg agctgttcac
     2281 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     2341 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     2401 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgca
     2461 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     2521 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     2581 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     2641 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     2701 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     2761 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     2821 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa
     2881 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     2941 cactctcggc atggacgagc tgtacaagta agaattcgat atcaagctta tcgataatca
     3001 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     3061 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     3121 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     3181 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     3241 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     3301 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     3361 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     3421 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     3481 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     3541 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc
     3601 tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga
     3661 ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt
     3721 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     3781 ggggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat
     3841 ctaccacaca caaggctact tccctgattg gcagaactac acaccagggc cagggatcag
     3901 atatccactg acctttggat ggtgctacaa gctagtacca gttgagcaag agaaggtaga
     3961 agaagccaat gaaggagaga acacccgctt gttacaccct gtgagcctgc atgggatgga
     4021 tgacccggag agagaagtat tagagtggag gtttgacagc cgcctagcat ttcatcacat
     4081 ggcccgagag ctgcatccgg actgtactgg gtctctctgg ttagaccaga tctgagcctg
     4141 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     4201 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     4261 cttttagtca gtgtggaaaa tctctagcag ggcccgttta aacccgctga tcagcctcga
     4321 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     4381 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     4441 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     4501 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     4561 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
     4621 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     4681 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     4741 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     4801 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     4861 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     4921 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     4981 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
     5041 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     5101 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     5161 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     5221 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     5281 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     5341 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     5401 tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag
     5461 gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac cgcgcgcgac
     5521 gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag
     5581 gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac
     5641 caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac
     5701 gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc
     5761 gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc
     5821 gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga ttccaccgcc
     5881 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     5941 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
     6001 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     6061 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
     6121 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     6181 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     6241 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     6301 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     6361 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     6421 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     6481 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     6541 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     6601 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     6661 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     6721 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     6781 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     6841 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     6901 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     6961 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     7021 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     7081 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     7141 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     7201 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     7261 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     7321 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     7381 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     7441 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     7501 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     7561 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     7621 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     7681 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     7741 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     7801 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     7861 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     7921 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     7981 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     8041 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     8101 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     8161 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     8221 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     8281 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt
     8341 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
     8401 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
     8461 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
     8521 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
     8581 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     8641 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     8701 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     8761 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
     8821 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
     8881 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
     8941 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
     9001 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
     9061 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
     9121 gcgcgttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     9181 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     9241 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     9301 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
     9361 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
     9421 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
     9481 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
     9541 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
     9601 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
     9661 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
     9721 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     9781 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc
     9841 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
     9901 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     9961 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
    10021 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
    10081 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
    10141 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
    10201 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
    10261 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
    10321 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
    10381 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
    10441 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
    10501 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
    10561 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
    10621 gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag atccattcga
    10681 ttagtgaacg gatcggcact gcgtgcgcca attctgcaga caaatggcag tattcatcca
    10741 caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
    10801 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
    10861 tcgggtttat taca
//
