LOCUS       sequence-211914-       18601 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ""CoinFLP-LexGAD/Gal4"" - Expresses LexGAD or Gal4 from actin 
            promoter downsteam of transcriptional stop. Recombination by FLP 
            excises FRT-FRT pair to express LexGAD, or FRT3-FRT3 pair to express
            Gal4..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18601)
  AUTHORS   Bosch JA, Tran NH, Hariharan IK
  TITLE     CoinFLP: a system for efficient mosaic screening and for visualizing
            clonal boundaries in Drosophila.
  JOURNAL   Development. 2015 Feb 1;142(3):597-606. doi: 10.1242/dev.114603.
  PUBMED    25605786
REFERENCE   2  (bases 1 to 18601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2015-02-1- 1"; volume: "142"; 
            issue: "3"; pages: "597-606"
COMMENT     Sequence Label: pAct-FRT-FRT3-stop-FRT-LexGAD-FRT3-Gal4 attB
FEATURES             Location/Qualifiers
     source          1..18601
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     121..143
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     135..152
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     136..152
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     159..178
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        159..177
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(211..229)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(681..752,1154..1427,1502..2156,2218..2533,2737..2868,
                     2939..3553)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    4328..4361
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    8601..8634
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    8655..8688
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     protein_bind    8978..9011
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             9028..9048
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(9058..9309,9310..9663)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     protein_bind    12152..12185
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     CDS             12265..14910
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4"
                     /product="GAL4 transcriptional activator"
                     /label=GAL4
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSIDSAAHHDNSTIPLDF
                     MPRDALHGFDWSEEDDMSDGLPFLKTDPNNNGFFGDGSLLCILRSIGFKPENYTNSNVN
                     RLPTMITDRYTLASRSTTSRLLQSYLNNFHPYCPIVHSPTLMMLYNNQIEIASKDQWQI
                     LFNCILAIGAWCIEGESTDIDVFYYQNAKSHLTSKVFESGSIILVTALHLLSRYTQWRQ
                     KTNTSYNFHSFSIRMAISLGLNRDLPSSFSDSSILEQRRRIWWSVYSWEIQLSLLYGRS
                     IQLSQNTISFPSSVDDVQRTTTGPTIYHGIIETARLLQVFTKIYELDKTVTAEKSPICA
                     KKCLMICNEIEEVSRQAPKFLQMDISTTALTNLLKEHPWLSFTRFELKWKQLSLIIYVL
                     RDFFTNFTQKKSQLEQDQNDHQSYEVKRCSIMLSDAAQRTVMSVSSYMDNHNVTPYFAW
                     NCSYYLFNAVLVPIKTLLSNSKSNAENNETAQLLQQINTVLMLLKKLATFKIQTCEKYI
                     QVLEEVCAPFLLSQCAIPLPHISYNNSNGSAIKNIVGSATIAQYPTLPEENVNNISVKY
                     VSPGSVGPSPVPLKSGASFSDLVKLLSNRPPSRNSPVTIPRSTPSHRSVTPFLGQQQQL
                     QSLVPLTPSALFGGANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     intron          15000..15065
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             15195..15215
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    15487..15621
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(15524..15543)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     15578..15597
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    15661..15730
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(15949..15967)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(15988..16004)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15988..16004)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(16001..16023)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    16012..16028
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(16036..16042,16043..16060,16061..16066))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    16081..16102
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(16219..16236)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(16390..16978)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(16470..16489)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(17149..17940,17941..18009))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     17772..17791
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(18010..18114)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(18140..18595)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(18277..18298)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     18489..18508
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
       61 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
      121 cccagtcacg acgttgtaaa acgacggcca gtgaattgta atacgactca ctatagggcg
      181 aattgggtac gtaccgggcc cctagtatgt atgtaagtta ataaaaccca tttttgcgga
      241 aagtagataa aaaaaacatt tttttttttt actgcactgg atatcattga acttatctga
      301 tcagttttaa atttacttcg atccaagggt atttgatgta ccaggttctt tcgattacct
      361 ctcactcaaa atgacattcc actcaaagtc agcgctgttt gcctccttct ctgtccacag
      421 aaatatcgcc gtctctttcg ccgctgcgtc cgctatctct ttcgccaccg tttgtagcgt
      481 tacgtagcgt caatgtccgc cttcagttgc attttgtcag cggtttcgtg acgaagctcc
      541 aagcggttta cgccatcaat taaacacaaa gtgctgtgcc aaaactcctc tcgcttctta
      601 tttttgtttg ttttttgagt gattggggtg gtgattggtt ttgggtgggt aagcagggga
      661 aagtgtgaaa aatcccggca atgggccaag aggatcagga gctattaatt cgcggaggca
      721 gcaaacaccc atctgccgag catctgaaca atgtgagtag tacatgtgca tacatcttaa
      781 gttcacttga tctataggaa ctgcgattgc aacatcaaat tgtctgcggc gtgagaactg
      841 cgacccacaa aaatcccaaa ccgcaattgc acaaacaaat agtgacacga aacagattat
      901 tctggtagct gttctcgcta tataagacaa tttttgagat catatcatga tcaagacatc
      961 taaaggcatt cattttcgac tatattcttt tttacaaaaa atataacaac cagatatttt
     1021 aagctgatcc tagatgcaca aaaaataaat aaaagtataa acctacttcg taggatactt
     1081 cggggtactt tttgttcggg gttagatgag cataacgctt gtagttgata tttgagatcc
     1141 cctatcattg cagggtgaca gcggagcggc ttcgcagagc tgcattaacc agggcttcgg
     1201 gcaggccaaa aactacggca cgctccggcc acccagtccg ccggaggact ccggttcagg
     1261 gagcggccaa ctagccgaga acctcaccta tgcctggcac aatatggaca tctttggggc
     1321 ggtcaatcag ccgggctccg gatggcggca gctggtcaac cggacacgcg gactattctg
     1381 caacgagcga cacataccgg cgcccaggaa acatttgctc aagaacggtg agtttctatt
     1441 cgcagtcggc tgatctgtgt gaaatcttaa taaagggtcc aattaccaat ttgaaactca
     1501 gtttgcggcg tggcctatcc gggcgaactt ttggccgtga tgggcagttc cggtgccgga
     1561 aagacgaccc tgctgaatgc ccttgccttt cgatcgccgc agggcatcca agtatcgcca
     1621 tccgggatgc gactgctcaa tggccaacct gtggacgcca aggagatgca ggccaggtgc
     1681 gcctatgtcc agcaggatga cctctttatc ggctccctaa cggccaggga acacctgatt
     1741 ttccaagcca tggtgcggat gccacgacat ctgacctatc ggcagcgagt ggcccgcgtg
     1801 gatcaggtga tccaggagct ttcgctcagc aaatgtcagc acacgatcat cggtgtgccc
     1861 ggcagggtga aaggtctgtc cggcggagaa aggaagcgtc tggcattcgc ctccgaggct
     1921 ctaaccgatc cgccgcttct gatctgcgat gagcccacct ccggactgga ctcctttacc
     1981 gcccacagcg tcgtccaggt gctgaagaag ctgtcgcaga agggcaagac cgtcatcctg
     2041 accattcatc agccgtcttc cgagctgttt gagctctttg acaagatcct tctgatggcc
     2101 gagggcaggg tagctttctt gggcactccc agcgaagccg tcgacttctt ttcctagtga
     2161 gttcgatgtg tttattaagg gtatctagta ttacataaca tctcaactcc tatccagcgt
     2221 gggtgcccag tgtcctacca actacaatcc ggcggacttt tacgtacagg tgttggccgt
     2281 tgtgcccgga cgggagatcg agtcccgtga tcggatcgcc aagatatgcg acaattttgc
     2341 cattagcaaa gtagcccggg atatggagca gttgttggcc accaaaaatc tggagaagcc
     2401 actggagcag ccggagaatg ggtacaccta caaggccacc tggttcatgc agttccgggc
     2461 ggtcctgtgg cgatcctggc tgtcggtgct caaggaacca ctcctcgtaa aagtgcgact
     2521 tattcagaca acggtgagtg gttccagtgg aaacaaatga tataacgctt acaattcttg
     2581 gaaacaaatt cgctagattt tagatagaat tgcctgattc cacacccttc ttagtttttt
     2641 tcaatgagat gtatagttta tagttttgca gaagataaat aaatttcatt taactcgcga
     2701 atattaatga gatgcgagta acattttaat ttgcagatgg ttgccatctt gattggcctc
     2761 atctttttgg gccaacaact cacgcaagtg ggtgtgatga atatcaacgg agccatcttc
     2821 ctcttcctga ccaacatgac ctttcaaaac gtctttgcca cgataaatgt aagtcatgtt
     2881 tagaatacat ttgcatttca ataatttact aactttctaa tgaatcgatt cgatttaggt
     2941 gttcacctca gagctgccag tttttatgag ggaggcccga agtcgacttt atcgctgtga
     3001 cacatacttt ctgggcaaaa cgattgccga attgccgctt tttctcacag tgccactggt
     3061 cttcacggcg attgcctatc cgatgatcgg actgcgggcc ggagtgctgc acttcttcaa
     3121 ctgcctggcg ctggtcactc tggtggccaa tgtgtcaacg tccttcggat atctaatatc
     3181 ctgcgccagc tcctcgacct cgatggcgct gtctgtgggt ccgccggtta tcataccatt
     3241 cctgctcttt ggcggcttct tcttgaactc gggctcggtg ccagtatacc tcaaatggtt
     3301 gtcgtacctc tcatggttcc gttacgccaa cgagggtctg ctgattaacc aatgggcgga
     3361 cgtggagccg ggcgaaatta gctgcacatc gtcgaacacc acgtgcccca gttcgggcaa
     3421 ggtcatcctg gagacgctta acttctccgc cgccgatctg ccgctggact acgtgggtct
     3481 ggccattctc atcgtgagct tccgggtgct cgcatatctg gctctaagac ttcgggcccg
     3541 acgcaaggag tagccgacat atatccgaaa taactgcttg tttttttttt ttaccattat
     3601 taccatcgtg tttactgttt attgccccct caaaaagcta atgtaattat atttgtgcca
     3661 ataaaaacaa gatatgacct atagaataca agtatttccc cttcgaacat ccccacaagt
     3721 agactttgga tttgtcttct aaccaaaaga cttacacacc tgcatacctt acatcaaaaa
     3781 ctcgtttatc gctacataaa acaccgggat atatttttta tatacatact tttcaaatcg
     3841 cgcgccctct tcataattca cctccaccac accacgtttc gtagttgctc tttcgctgtc
     3901 tcccacccgc tctccgcaac acattcacct tttgttcgac gaccttggag cgactgtcgt
     3961 tagttccgcg cgattcggtt cgctcaaatg gttccgagtg gttcatttcg tctcaataga
     4021 aattagtaat aaatatttgt atgtacaatt tatttgctcc aatatatttg tatatatttc
     4081 cctcacagct atatttattc taatttaata ttatgacttt ttaaggtaat tttttgtgac
     4141 ctgttcggag tgattagcgt tacaatttga actgaaagtg acatccagtg tttgttcctt
     4201 gtgtagatgc atctcaaaaa aatggtgggc ataatagtgt tgtttatata tatcaaaaat
     4261 aacaactata ataataagaa tacatttaat ttagaaaatg cttggatttc actggaacta
     4321 ggctagcata acttcgtata atgtatgcta tacgaagtta tgctagcgga tctcgctgcc
     4381 tgttatgtgg cccacaaacc aagacacgtt ttatggccat taaagctggc tgatcgtcgc
     4441 caaacaccaa atacataatg aatatgtaca cattcgagaa agaagcgatc aaagaagcgt
     4501 cttcgggcgg agtaggagaa tgcggaggag aaggagaacg agctgatcta gtatctctcc
     4561 acaatccaat gccaactgac caactggcca tattcggagc aatttgaagc caatttccat
     4621 cgcctggcga tcgctccatt cttggctata tgtttttcac cgttacccgg ggccattttc
     4681 aaagactcgt cggcaagata agattgtgtc actcgctgtc tctcttcatt tgtcgaagaa
     4741 tgctgaggaa tttcgcgatg acgtcggcga gtattttgaa gaatgagaat aatttgtatt
     4801 tatacgaaaa tcagttagtg gaattttcta caaaaacatg ttatctatag ataattttgt
     4861 tgcaaaatat gttgactatg acaaagattg tatgtatata cctttaatgt attctcattt
     4921 tcttatgtat ttataatggc aatgatgata ctgatgatat tttaagatga tgccagacca
     4981 aaaggcttga atttctgcgt cttttgccga acgcagtgca tgtgcaattg ttgttttttg
     5041 gaatattcaa ttttcggact gtccgctttg atttcagttt cttggcttat tcaaaaagca
     5101 aagtaaagcc aaaaaagcga gatggcaata ccaaatgcgg aaaacggtag tggaaggaaa
     5161 ggggtgcggg gcagcggaag gaagggtggg gcggggcgtg gcggggtctg tggctgggcg
     5221 cgacgtcacc gacgttggag ccactccttt gaccatgtgt gcgtgtgtgt attattcgtg
     5281 tctcgccact cgccggttgt ttttttcttt ttatgctgcg ctctctctag cgccatctcg
     5341 cttacgcatg ctcaacgcac cgcatgttgc cgtttccttt tatgcgtcat tttggctcga
     5401 aataggcaat tatttaaaca aagattagtc aacgaaaacg ctaaaataaa taagtctaca
     5461 atatggttac ttattgccat gtgtgtgcag ccaacgatag caacaaaagc aacaacacag
     5521 gtggctttcc ctctttcact ttttgtttgc aagccgcgtg cgagcaagac ggcacgaccg
     5581 gcaaacgcaa ttacgctgac aaagagcaga cgaagttttg gcgaaaaaca tcaaggcgcc
     5641 tgatacgaat gcatttgcaa taacaattgc gatatttaat attgtttatg aagctgtttg
     5701 acttcaaaac acacaaaaaa aaaaataaaa caaattattt gaaagagaat taggaatcgg
     5761 acgcttatcg ttagggtaac aacaagaaat gcttactgag tcacagcctc tggaaaactg
     5821 ccgcaagcca gagagagaga gaaaaagagg gagagcagct tagaccgcat gtgcttgtgt
     5881 gtgaggcgtc tctctcttcg tctctgttgc gcaaacgcat agactgcact gaaaaaatcg
     5941 attacctatt ttttatgaat gaatatttgc actattacta ttcaaaacta ttaagatagc
     6001 aatcacattc aatagccaaa tactatacca cctgagcgat gcaacgaaat gatcaatttg
     6061 agcaaaaatg ctgcatattt aggacggcat cattatagaa atgcttcttg ctgtgtactt
     6121 ttctctcgtc tggcagctgt ttcgccgtta ttgttaaaac cggcttaagt taggtgtgtt
     6181 ttctacgact agtgaatgcc ctactagaag atgtgtgttg cacaaaatgt ccctggaata
     6241 accaatttga agtgcagata gcagtaaacg taagctaata tgaatattat ttaactgtaa
     6301 tgttttaata tcgctggaca ttactaataa acccactata aacacatgta catatgtatg
     6361 ttttggcata caatgagtag ttggggaaaa aatgtgtaaa agcaccgtga ccatcacagc
     6421 ataaagataa ccagctgaag tatcgaatat gagtaacccc caaattgaat cacatgccgc
     6481 aactgatagg acccatggaa gtacactctt catggcgata tacaagacac acacaagcac
     6541 gaacacccag ttgcggagga aattctccgt aaatgaaaac ccaatcggcg aacaattcat
     6601 acccatatat ggtaaaagtt ttgaacgcga cttgagagcg gagagcattg cggctgataa
     6661 ggttttagcg ctaagcgggc tttataaaac gggctgcggg accagttttc atatcactac
     6721 cgtttgagtt cttgtgctgt gtggatactc ctcccgacac aaagccgctc catcagccag
     6781 cagtcgtcta atccagagac accaaaccga aagacttaat ttatatttat ttaattaatt
     6841 ttaataaaac acaccaaatg taagtagctt tccccttccc aacaacaaaa caccatcgaa
     6901 ccactcccac caagaaaaag caataatcga gaaaagccgc ggaaaatgtg tgattttttt
     6961 tgtaaacaaa acattttttt atgtgccagt gctgaaagtg atcaaaaaat actagccacg
     7021 agctaaagag ttattgtatt gaccaaaact ccaaaaatac ccaagtttgg ccctaaattg
     7081 tcaatcaaaa taccaatagg tcgaaagaca tcaaaattaa caaaaccagg gtttcaaata
     7141 ccataactca agaatcagga ttacaactgc agatttcagg atatatacat acaaattata
     7201 cgaaattata aaaaccaaag caattcaata gccccaactc aaatgttagg atctaatata
     7261 gtgtttaaag ccaagctcgc tgatgtgggc gtgtcacgat ttcacccaaa gatatgccaa
     7321 attacgaatt gcaaatcaat tcgccaacac tttctttttt tcccacgccc taaaacacca
     7381 gatcatccat aaatgtacat acatacagta tatgcatatt ataatctgta aaactagatc
     7441 aggttcttga aaatagtgac gtaggcagcc gttttggctg aagcagaaat ttttgccggt
     7501 ttttcaaagt tgtagttgca aaaatggaga aaaccttcga gcattcgttc atatacacac
     7561 actcacgcgc aaaataacga gagagagtgt atgtgtgtgt gagagagcga aagccagacg
     7621 acggtttgct tttcgcctcg aaacatgacc atatatggtc acaaaacttg gccgccgcaa
     7681 ttcaacacac cagcgctctc cttcgcaccc atagcgacca tggcgcggag cgagcgagat
     7741 ggcgagagcg agcgacgcct atggcgacgt cgacgcaggc agcgattgaa aaacgcagtt
     7801 aactggcatt caacattcac cagccacttt cagtcggttt attccagtca ttcctttcaa
     7861 accgtgcggt cgcttagctc agcctcgcca cttgcgttta cagtagtttt cacgccttga
     7921 atttgttaaa tcgaacaaaa aggtaaagtt taactagctt tgaaaagttt cgtggctctt
     7981 aattgttaaa ttttctagag tgcgtttagt gttttttttt ttttttattt tgtaatgtta
     8041 atttcgggtt ccaattcgag ttttaggcag ccgccatttt aagggcgcat acacacaggc
     8101 aactgtgctc tctttgcggc tttcttttgc accggcattc gttaagtgtc gtctagaagc
     8161 ttctcccctc ccttttcggc atattcgtat tgtggtttta atttttcggg gcggggcctt
     8221 ctattttgta actgttcttt taatttctta ttacaattcg atcgcaagtg aaaatcagtt
     8281 ttcaatcgga aaagtatttt tttatgaaat ttttttttgt ccaagattaa aattttgtac
     8341 taaaaaaacg tacattgcat tgagtgattt ttaattgtac acgaaaaaca agttagtttg
     8401 ttatgacaat tgtactttgg tagaccagcg cagtccaagg aaaccacgca aattctcagt
     8461 tttttttttg ccatttctac attaccaaat aaggtaacca aaaactaatg ggaaatccgc
     8521 attctttcca ttgcagctta caggatcgat ccccgggtac ggctagtgga tcagcttgca
     8581 tgcctgcagg tcccctcgag gaagttccta ttctctagaa agtataggaa cttccccgcg
     8641 taccaccaga cagcgaagtt cctattcttc aaatagtata ggaacttcga attgactaaa
     8701 gccaaataga aaattattca gttcctggct taagttttta aaagtgatat tatttatttg
     8761 gttgtaacca accaaaagaa tgtaaataac taatacataa ttatgttagt tttaagttag
     8821 caacaaattg attttagcta tattagctac ttggttaata aatagaatat atttatttaa
     8881 agataattgc gtttttattg tcagggagtg agtttgctta aaaactcgtt tagatccact
     8941 agttctagtt gcggccggcc gcaactagag gatcccggaa gttcctattc tctagaaagt
     9001 ataggaactt cgaattccaa aatgccaccc aagaagaagc gaaaagtaga agatccaatg
     9061 aaggctctca cggcccgaca acaggaagtt tttgatttga tacgggatca tatatcccaa
     9121 acgggtatgc ctccgacccg cgcagagata gcacagcgac tgggctttcg atcgcctaac
     9181 gccgcggagg agcacttgaa ggcactggcc cgcaagggtg tcattgaaat cgtgtccggt
     9241 gcgagccgcg gaatccggct gttgcaggag gaggaagagg gcctgccact ggtgggacgc
     9301 gtggccgctg gcgagccgct gctggcccag caacacatag agggacacta tcaggtggac
     9361 ccctccttgt ttaagccaaa tgctgatttc ctgttgcggg tgtcgggaat gtccatgaag
     9421 gacatcggta ttatggatgg tgacctcctc gccgtccata agacacagga tgtcaggaac
     9481 ggccaggtag tcgttgccag gatagacgat gaggtcactg tgaaacgtct caagaagcaa
     9541 ggcaataagg tcgagctgct gccggagaat agcgagttca agccgatcgt ggtggatctg
     9601 cgacagcagt cctttactat cgagggcttg gccgtgggtg tgatccgcaa cggagattgg
     9661 ctgggatcca tcgacagtgc ggcccaccac gacaacagca ccattccact ggacttcatg
     9721 cctcgtgacg cactgcatgg atttgactgg agcgaggagg atgatatgag cgacggactc
     9781 ccattcctga agaccgaccc caataacaat ggtttctttg gcgacggcag cctgctgtgt
     9841 atcctgcgaa gtattggttt taagccggaa aattacacga acagtaatgt caatcgtctt
     9901 cccacaatga tcaccgaccg ttacacgctg gccagtcgca gtactacaag ccgcctgctg
     9961 caaagctatc ttaacaactt ccacccctac tgccccatag tgcatagtcc tactctcatg
    10021 atgctttaca acaatcagat tgagatagca tcgaaggacc agtggcagat cctcttcaac
    10081 tgcattctcg ccatcggcgc ttggtgtatt gaaggcgaga gcacagatat cgacgtgttt
    10141 tactatcaaa acgccaagtc gcacctcacc tcgaaggtct tcgaaagtgg aagcattatc
    10201 ctcgtgacag ccctccatct gctgagtcgt tatacccagt ggcgtcagaa aactaataca
    10261 tcctacaact ttcattcgtt cagcattcgg atggcgatta gcctgggact taatcgtgac
    10321 ctcccaagta gcttcagcga ttcgtcgatc ctggaacagc gccggaggat ttggtggagt
    10381 gtgtacagct gggagatcca gctgtccctc ttgtacggac gcagcattca gctgagtcag
    10441 aacacgattt ccttcccgtc cagcgtggac gacgttcaac ggacaactac gggcccgacc
    10501 atctaccacg gtatcattga gacggcccgc ttgcttcaag tgtttacgaa gatttatgag
    10561 ctggataaga cagtgaccgc ggagaagagt ccgatctgcg ccaagaaatg cctgatgatc
    10621 tgcaatgaga tagaggaggt gagccgccag gcacccaagt tcctgcagat ggacataagc
    10681 actacggcgc tgactaatct cctcaaagaa cacccctggc tgagtttcac ccgcttcgag
    10741 ctgaagtgga aacagctgag tctgattata tacgttttgc gggacttttt cacaaatttt
    10801 acgcagaaga agtcgcagct tgagcaagat cagaacgacc atcagtccta cgaggtaaag
    10861 aggtgcagca taatgctctc cgacgcagcc cagcgcaccg tgatgtcggt gtcgtcctat
    10921 atggacaacc ataatgtgac cccttatttc gcctggaatt gctcctacta tttgttcaac
    10981 gccgtgttgg tcccaatcaa gactcttctg agcaactcca aatcgaacgc cgagaataac
    11041 gagacagcac agctcttgca gcaaattaac accgtgttga tgctgttgaa gaaactcgct
    11101 acattcaaga ttcagacttg cgaaaagtac atccaggtgt tggaagaggt gtgcgcccca
    11161 ttccttcttt cgcaatgcgc aattcccctc ccgcacataa gttacaataa ttccaacggc
    11221 tccgctatca agaatatcgt cggctccgct acaatcgcgc agtatcccac gctgcctgag
    11281 gaaaatgtta acaacatatc ggtgaagtat gtaagtccag gcagcgtcgg cccgagccct
    11341 gtgccgctga aaagcggagc ctccttcagc gatctggtca agctgctcag caaccgccca
    11401 ccatcccgca actcccctgt cactataccc cgatcgaccc cgtcgcatcg tagcgttacc
    11461 ccattcctgg gacaacagca gcaactgcag agtctggtgc cacttacgcc ctcggcattg
    11521 tttggcggag ccaacttcaa ccagagtggt aacattgcgg acagctcctt gagcttcacg
    11581 tttaccaaca gctccaacgg tccaaacttg atcacgactc agacaaactc ccaggcattg
    11641 tcccagccca ttgccagctc gaatgtgcat gataacttta tgaataatga aattactgcc
    11701 tccaagattg acgacggcaa caacagcaag cccctttccc caggctggac tgaccagacg
    11761 gcctacaatg ctttcggtat caccactggc atgttcaaca ccacaacgat ggacgacgta
    11821 tataactatc tttttgatga tgaggatact ccaccgaacc caaagaagga gtaggtttaa
    11881 acaagcttat cgataccgtc gactaaagcc aaatagaaaa ttattcagtt cctggcttaa
    11941 gtttttaaaa gtgatattat ttatttggtt gtaaccaacc aaaagaatgt aaataactaa
    12001 tacataatta tgttagtttt aagttagcaa caaattgatt ttagctatat tagctacttg
    12061 gttaataaat agaatatatt tatttaaaga taattgcgtt tttattgtca gggagtgagt
    12121 ttgcttaaaa actcgtttag atcgcggccg cgaagttcct attcttcaaa tagtatagga
    12181 acttcagatc ttaagcaaat aaacaagcgc agctgaacaa gctaaacaat ctgcagccca
    12241 agcttgaagc aagcctcctg aaagatgaag ctactgtctt ctatcgaaca agcatgcgat
    12301 atttgccgac ttaaaaagct caagtgctcc aaagaaaaac cgaagtgcgc caagtgtctg
    12361 aagaacaact gggagtgtcg ctactctccc aaaaccaaaa ggtctccgct gactagggca
    12421 catctgacag aagtggaatc aaggctagaa agactggaac agctatttct actgattttt
    12481 cctcgagaag accttgacat gattttgaaa atggattctt tacaggatat aaaagcattg
    12541 ttaacaggat tatttgtaca agataatgtg aataaagatg ccgtcacaga tagattggct
    12601 tcagtggaga ctgatatgcc tctaacattg agacagcata gaataagtgc gacatcatca
    12661 tcggaagaga gtagtaacaa aggtcaaaga cagttgactg tatcgattga ctcggcagct
    12721 catcatgata actccacaat tccgttggat tttatgccca gggatgctct tcatggattt
    12781 gattggtctg aagaggatga catgtcggat ggcttgccct tcctgaaaac ggaccccaac
    12841 aataatgggt tctttggcga cggttctctc ttatgtattc ttcgatctat tggctttaaa
    12901 ccggaaaatt acacgaactc taacgttaac aggctcccga ccatgattac ggatagatac
    12961 acgttggctt ctagatccac aacatcccgt ttacttcaaa gttatctcaa taattttcac
    13021 ccctactgcc ctatcgtgca ctcaccgacg ctaatgatgt tgtataataa ccagattgaa
    13081 atcgcgtcga aggatcaatg gcaaatcctt tttaactgca tattagccat tggagcctgg
    13141 tgtatagagg gggaatctac tgatatagat gttttttact atcaaaatgc taaatctcat
    13201 ttgacgagca aggtcttcga gtcaggttcc ataattttgg tgacagccct acatcttctg
    13261 tcgcgatata cacagtggag gcagaaaaca aatactagct ataattttca cagcttttcc
    13321 ataagaatgg ccatatcatt gggcttgaat agggacctcc cctcgtcctt cagtgatagc
    13381 agcattctgg aacaaagacg ccgaatttgg tggtctgtct actcttggga gatccaattg
    13441 tccctgcttt atggtcgatc catccagctt tctcagaata caatctcctt cccttcttct
    13501 gtcgacgatg tgcagcgtac cacaacaggt cccaccatat atcatggcat cattgaaaca
    13561 gcaaggctct tacaagtttt cacaaaaatc tatgaactag acaaaacagt aactgcagaa
    13621 aaaagtccta tatgtgcaaa aaaatgcttg atgatttgta atgagattga ggaggtttcg
    13681 agacaggcac caaagttttt acaaatggat atttccacca ccgctctaac caatttgttg
    13741 aaggaacacc cttggctatc ctttacaaga ttcgaactga agtggaaaca gttgtctctt
    13801 atcatttatg tattaagaga ttttttcact aattttaccc agaaaaagtc acaactagaa
    13861 caggatcaaa atgatcatca aagttatgaa gttaaacgat gctccatcat gttaagcgat
    13921 gcagcacaaa gaactgttat gtctgtaagt agctatatgg acaatcataa tgtcacccca
    13981 tattttgcct ggaattgttc ttattacttg ttcaatgcag tcctagtacc cataaagact
    14041 ctactctcaa actcaaaatc gaatgctgag aataacgaga ccgcacaatt attacaacaa
    14101 attaacactg ttctgatgct attaaaaaaa ctggccactt ttaaaatcca gacttgtgaa
    14161 aaatacattc aagtactgga agaggtatgt gcgccgtttc tgttatcaca gtgtgcaatc
    14221 ccattaccgc atatcagtta taacaatagt aatggtagcg ccattaaaaa tattgtcggt
    14281 tctgcaacta tcgcccaata ccctactctt ccggaggaaa atgtcaacaa tatcagtgtt
    14341 aaatatgttt ctcctggctc agtagggcct tcacctgtgc cattgaaatc aggagcaagt
    14401 ttcagtgatc tagtcaagct gttatctaac cgtccaccct ctcgtaactc tccagtgaca
    14461 ataccaagaa gcacaccttc gcatcgctca gtcacgcctt ttctagggca acagcaacag
    14521 ctgcaatcat tagtgccact gaccccgtct gctttgtttg gtggcgccaa ttttaatcaa
    14581 agtgggaata ttgctgatag ctcattgtcc ttcactttca ctaacagtag caacggtccg
    14641 aacctcataa caactcaaac aaattctcaa gcgctttcac aaccaattgc ctcctctaac
    14701 gttcatgata acttcatgaa taatgaaatc acggctagta aaattgatga tggtaataat
    14761 tcaaaaccac tgtcacctgg ttggacggac caaactgcgt ataacgcgtt tggaatcact
    14821 acagggatgt ttaataccac tacaatggat gatgtatata actatctatt cgatgatgaa
    14881 gataccccac caaacccaaa aaaagagtaa ggtacctcta gaggatcttt gtgaaggaac
    14941 cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg
    15001 taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat
    15061 tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg
    15121 aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc
    15181 aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag
    15241 aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta
    15301 tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatatttga
    15361 tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga ggttttactt
    15421 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
    15481 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
    15541 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
    15601 gtatcttatc atgtctggat ccactagaga tccactagtg tcgacgatgt aggtcacggt
    15661 ctcgaagccg cggtgcgggt gccagggcgt gcccttgggc tccccgggcg cgtactccac
    15721 ctcacccatc tggtccatca tgatgaacgg gtcgaggtgg cggtagttga tcccggcgaa
    15781 cgcgcggcgc accgggaagc cctcgccctc gaaaccgctg ggcgcggtgg tcacggtgag
    15841 cacgggacgt gcgacggcgt cggcgggtgc ggatacgcgg ggcagcgtca gcgggttctc
    15901 gacggtcacg gcgggcatgt cgacactagt tctagccagc ttttgttccc tttagtgagg
    15961 gttaatttcg agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
    16021 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
    16081 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
    16141 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
    16201 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
    16261 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
    16321 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
    16381 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
    16441 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
    16501 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
    16561 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
    16621 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
    16681 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
    16741 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    16801 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
    16861 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    16921 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    16981 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    17041 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
    17101 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
    17161 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
    17221 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
    17281 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
    17341 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    17401 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    17461 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    17521 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    17581 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    17641 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    17701 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    17761 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    17821 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    17881 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    17941 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    18001 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    18061 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    18121 tccccgaaaa gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat
    18181 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
    18241 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
    18301 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
    18361 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
    18421 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
    18481 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
    18541 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat
    18601 t
//
