LOCUS       sequence-211922-        8914 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses USP1_+_62903144.23-P1P2 sgRNA in pCR1068.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8914)
  AUTHORS   Richardson CD, Kazane KR, Feng SJ, Zelin E, Bray NL, Schafer AJ, 
            Floor SN, Corn JE
  TITLE     CRISPR-Cas9 genome editing in human cells occurs via the Fanconi 
            anemia pathway.
  JOURNAL   Nat Genet. 2018 Aug;50(8):1132-1139. doi: 10.1038/s41588-018-0174-0.
            Epub 2018 Jul 27.
  PUBMED    30054595
REFERENCE   2  (bases 1 to 8914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41588-018-0174-0"; journalName: "Nat Genet"; date: 
            "2018-08"; volume: "50"; issue: "8"; pages: "1132-1139"
COMMENT     Sequence Label: pCR1300
FEATURES             Location/Qualifiers
     source          1..8914
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        30..409
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        411..609
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     564..584
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             627..807
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    854..979
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1476..1709
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1894..1938
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2236..2353
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(2614..2647)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     2651..2671
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     promoter        2924..3135
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          3136..4074
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4031..4051
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4095..4691
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4095..4114)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4591..4611
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4701..4754
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      4761..4770
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4767..4769,4770..4772,4773..5486)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4812..4833)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5073..5092)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5420..5441
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(5506..5539)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    5595..6183
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5648..5668)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6066..6077)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6186..6202)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6187..6203)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6712..6892
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(6954..7542)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7034..7053)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7713..8504,8505..8573))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8336..8355
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8574..8678)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8753..8772)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta
       61 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
      121 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
      181 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
      241 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
      301 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
      361 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
      421 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
      481 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
      541 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
      601 tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg
      661 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
      721 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
      781 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
      841 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
      901 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
      961 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
     1021 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     1081 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     1141 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     1201 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     1261 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     1321 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
     1381 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
     1441 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
     1501 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
     1561 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
     1621 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
     1681 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
     1741 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
     1801 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
     1861 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
     1921 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
     1981 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
     2041 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
     2101 ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac
     2161 agatccattc gattagtgaa cggatcggca ctgcgtgcgc caattctgca gacaaatggc
     2221 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     2281 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
     2341 aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttggt tagtaccggg
     2401 cccgccctgc aggtctagag atccgacgcg ccatctctag gcccgcgccg gccccctcgc
     2461 acggacttgt gggagaagct cggctactcc cctgccccgg ttaatttgca tataatattt
     2521 cctagtaact atagaggctt aatgtgcgat aaaagacaga taatctgttc tttttaatac
     2581 tagctacatt ttacatgata ggcttggatt tctataactt cgtatagcat acattatacg
     2641 aagttataaa cagcacaaaa ggaaactcac cctaactgta aagtaattgt gtgttttgag
     2701 actataagta tcccttggag aaccaccttg ttggcacggt cccggggtct cccgtttaag
     2761 agctaagctg gaaacagcat agcaagttta aataaggcta gtccgttatc aacttgaaaa
     2821 agtggcaccg agtcggtgct ttttttctcg agtactagga tccattaggc ggccgccctg
     2881 cagggtggat aaccgtatta ccgccatgca tgtgcccgtc agtgggcaga gcgcacatcg
     2941 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg
     3001 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt
     3061 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt
     3121 gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt
     3181 tatggccctt gcgtgccttg aattacttcc acctggctgc agtacgtgat tcttgatccc
     3241 gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg agccccttcg
     3301 cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg
     3361 caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga
     3421 cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca agatctgcac
     3481 actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca
     3541 tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa
     3601 gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg
     3661 gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct
     3721 gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc
     3781 acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac
     3841 cgggcgccgt ccaggcacct cgattagttc tcgcgctttt ggagtacgtc gtctttaggt
     3901 tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt
     3961 aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct
     4021 tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtgtcg
     4081 tgagctagcc caccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     4141 tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     4201 ccgtcgaccc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     4261 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     4321 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     4381 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     4441 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
     4501 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     4561 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     4621 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     4681 agcccggtgc cggatcggga gagggcagag gaagtctgct aacatgcggt gacgtcgagg
     4741 agaatcctgg cccaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
     4801 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     4861 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     4921 gcaagctgcc ggtgccctgg cccaccctcg tgaccaccct gacccatggc gtgcagtgct
     4981 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     5041 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     5101 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     5161 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     5221 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     5281 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     5341 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccaagctg agtaaagacc
     5401 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     5461 tcggcatgga cgagctgtac aagtaagaat tcgtcgaggg acctaataac ttcgtatagc
     5521 atacattata cgaagttata catgtttaag ggttccggtt ccactaggta caattcgata
     5581 tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     5641 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     5701 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     5761 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     5821 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     5881 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     5941 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     6001 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     6061 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     6121 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     6181 cgcatcgata ccgtcgacct cgatcgagac ctagaaaaac atggagcaat cacaagtagc
     6241 aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg
     6301 ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat
     6361 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     6421 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttggcagaac
     6481 tacacaccag ggccagggat cagatatcca ctgacctttg gatggtgcta caagctagta
     6541 ccagttgagc aagagaaggt agaagaagcc aatgaaggag agaacacccg cttgttacac
     6601 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tattagagtg gaggtttgac
     6661 agccgcctag catttcatca catggcccga gagctgcatc cggactgtac tgggtctctc
     6721 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     6781 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     6841 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagcatgtga
     6901 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     6961 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     7021 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     7081 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     7141 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     7201 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     7261 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     7321 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     7381 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     7441 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     7501 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     7561 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     7621 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     7681 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     7741 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     7801 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     7861 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     7921 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     7981 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     8041 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     8101 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     8161 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     8221 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     8281 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     8341 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     8401 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     8461 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     8521 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     8581 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     8641 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     8701 cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct
     8761 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg
     8821 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga
     8881 agaatctgct tagggttagg cgttttgcgc tgct
//
