LOCUS       sequence-211953-        8914 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses XRCC3_+_104181786.23-P1P2 sgRNA in pCR1068.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8914)
  AUTHORS   Richardson CD, Kazane KR, Feng SJ, Zelin E, Bray NL, Schafer AJ, 
            Floor SN, Corn JE
  TITLE     CRISPR-Cas9 genome editing in human cells occurs via the Fanconi 
            anemia pathway.
  JOURNAL   Nat Genet. 2018 Aug;50(8):1132-1139. doi: 10.1038/s41588-018-0174-0.
            Epub 2018 Jul 27.
  PUBMED    30054595
REFERENCE   2  (bases 1 to 8914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41588-018-0174-0"; journalName: "Nat Genet"; date: 
            "2018-08"; volume: "50"; issue: "8"; pages: "1132-1139"
COMMENT     Sequence Label: pCR1281
FEATURES             Location/Qualifiers
     source          1..8914
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        14..393
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        395..593
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     548..568
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             611..791
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    838..963
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1460..1693
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1878..1922
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2220..2337
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(2598..2631)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     2635..2655
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     promoter        2908..3119
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          3120..4058
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4015..4035
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4079..4675
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4079..4098)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4575..4595
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4685..4738
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      4745..4754
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4751..4753,4754..4756,4757..5470)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4796..4817)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5057..5076)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5404..5425
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(5490..5523)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    5579..6167
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5632..5652)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6050..6061)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6170..6186)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6171..6187)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6696..6876
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(6938..7526)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7018..7037)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7697..8488,8489..8557))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8320..8339
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8558..8662)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8737..8756)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 agatatacgc gttgacattg attattgact agttattaat agtaatcaat tacggggtca
       61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      541 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagcgcgttt
      601 tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
      661 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
      721 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
      781 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
      841 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
      901 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
      961 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
     1021 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
     1081 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
     1141 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     1201 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
     1261 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccggccg ctgatcttca
     1321 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     1381 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     1441 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     1501 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     1561 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     1621 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     1681 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     1741 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     1801 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     1861 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     1921 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     1981 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     2041 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca
     2101 ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag
     2161 tgaacggatc ggcactgcgt gcgccaattc tgcagacaaa tggcagtatt catccacaat
     2221 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     2281 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg
     2341 gtttattaca gggacagcag agatccagtt tggttagtac cgggcccgcc ctgcaggtct
     2401 agagatccga cgcgccatct ctaggcccgc gccggccccc tcgcacggac ttgtgggaga
     2461 agctcggcta ctcccctgcc ccggttaatt tgcatataat atttcctagt aactatagag
     2521 gcttaatgtg cgataaaaga cagataatct gttcttttta atactagcta cattttacat
     2581 gataggcttg gatttctata acttcgtata gcatacatta tacgaagtta taaacagcac
     2641 aaaaggaaac tcaccctaac tgtaaagtaa ttgtgtgttt tgagactata agtatccctt
     2701 ggagaaccac cttgttgggc cgagaggagc aggacgggtt taagagctaa gctggaaaca
     2761 gcatagcaag tttaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg
     2821 tgcttttttt ctcgagtact aggatccatt aggcggccgc cctgcagggt ggataaccgt
     2881 attaccgcca tgcatgtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga
     2941 agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact
     3001 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata
     3061 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg
     3121 taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc
     3181 cttgaattac ttccacctgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa
     3241 gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg
     3301 aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc
     3361 tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt
     3421 ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt atttcggttt
     3481 ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg
     3541 gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc
     3601 tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt
     3661 cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa
     3721 aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg
     3781 cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc
     3841 acctcgatta gttctcgcgc ttttggagta cgtcgtcttt aggttggggg gaggggtttt
     3901 atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca gcttggcact
     3961 tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc
     4021 ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagct agcccaccat
     4081 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg
     4141 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg acccggaccg
     4201 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     4261 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     4321 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     4381 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     4441 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     4501 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     4561 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     4621 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgccggatc
     4681 gggagagggc agaggaagtc tgctaacatg cggtgacgtc gaggagaatc ctggcccacc
     4741 ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     4801 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     4861 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgccggtgcc
     4921 ctggcccacc ctcgtgacca ccctgaccca tggcgtgcag tgcttcagcc gctaccccga
     4981 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     5041 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     5101 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     5161 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
     5221 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
     5281 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     5341 cgacaaccac tacctgagca cccagtccaa gctgagtaaa gaccccaacg agaagcgcga
     5401 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     5461 gtacaagtaa gaattcgtcg agggacctaa taacttcgta tagcatacat tatacgaagt
     5521 tatacatgtt taagggttcc ggttccacta ggtacaattc gatatcaagc ttatcgataa
     5581 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     5641 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     5701 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     5761 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     5821 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     5881 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     5941 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     6001 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     6061 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     6121 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg
     6181 acctcgatcg agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca
     6241 atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac
     6301 ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     6361 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc
     6421 tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag
     6481 ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagcaagaga
     6541 aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg agcctgcatg
     6601 ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc ctagcatttc
     6661 atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta gaccagatct
     6721 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     6781 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     6841 tcagaccctt ttagtcagtg tggaaaatct ctagcagcat gtgagcaaaa ggccagcaaa
     6901 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     6961 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     7021 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     7081 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     7141 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     7201 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     7261 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     7321 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     7381 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     7441 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     7501 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     7561 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     7621 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     7681 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     7741 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     7801 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     7861 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     7921 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     7981 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     8041 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     8101 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     8161 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     8221 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     8281 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     8341 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     8401 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     8461 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     8521 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     8581 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     8641 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
     8701 cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata
     8761 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa
     8821 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt
     8881 taggcgtttt gcgctgcttc gcgatgtacg ggcc
//
