LOCUS       sequence-211957-       11260 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FANCF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11260)
  AUTHORS   Richardson CD, Kazane KR, Feng SJ, Zelin E, Bray NL, Schafer AJ, 
            Floor SN, Corn JE
  TITLE     CRISPR-Cas9 genome editing in human cells occurs via the Fanconi 
            anemia pathway.
  JOURNAL   Nat Genet. 2018 Aug;50(8):1132-1139. doi: 10.1038/s41588-018-0174-0.
            Epub 2018 Jul 27.
  PUBMED    30054595
REFERENCE   2  (bases 1 to 11260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41588-018-0174-0"; journalName: "Nat Genet"; date: 
            "2018-08"; volume: "50"; issue: "8"; pages: "1132-1139"
COMMENT     Sequence Label: pCR1446
FEATURES             Location/Qualifiers
     source          1..11260
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        21..125
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(126..194,195..986)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(344..363)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1157..1745
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1646..1665
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1899..1916
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2033..2054
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2069..2074,2075..2092,2093..2099)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2107..2123
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2112..2134
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2131..2147
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2131..2147
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2166..2186
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2168..2186
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2214..2440
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2441..2621
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2668..2793
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3286..3519
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3704..3748
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4015..4131
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4165..4189
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4320..5498
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4551..5489
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5446..5466
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        5515..5533
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5581..5607
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(6930..6947)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7157..7176
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      7332..7341
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7344..7832
                     /codon_start=1
                     /product="mouse GPI-anchored cell surface glycoprotein of 
                     the immunoglobulin superfamily"
                     /label=Thy1.1
                     /note="This allele of Thy1 is also known as CD90.1."
                     /translation="MNPAISVALLLSVLQVSRGQKVTSLTACLVNQNLRLDCRHENNTK
                     DNSIQHEFSLTREKRKHVLSGTLGIPEHTYRSRVTLSNQPYIKVLTLANFTTKDEGDYF
                     CELRVSGANPMSSNKSISVYRDKLVKCGGISLLVQNTSWMLLLLLSLSLLQALDFISL"
     protein_bind    complement(7838..7862)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    7893..8481
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7946..7966)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8364..8375)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        8532..9031
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8567..8590)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8964..8983
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             9045..9848
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9108..9127)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9718..9737
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             9992..10225
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10297..10418
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10334..10353)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10388..10407
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10458..10593
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(10614..10632)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10642..10659)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10642..10658)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10651..10673)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10800..11255
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10887..10906)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11097..11118
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
       61 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      121 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      181 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
      241 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
      301 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
      361 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
      421 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
      481 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
      541 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
      601 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
      661 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
      721 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
      781 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
      841 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
      901 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
      961 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     1021 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     1081 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     1141 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1201 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     1261 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     1321 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     1381 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     1441 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     1501 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     1561 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     1621 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     1681 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     1741 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     1801 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     1861 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     1921 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     1981 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     2041 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     2101 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     2161 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc
     2221 ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca
     2281 aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt
     2341 attaggaagg caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg
     2401 cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca
     2461 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     2521 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     2581 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
     2641 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
     2701 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
     2761 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
     2821 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
     2881 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     2941 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     3001 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     3061 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     3121 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     3181 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     3241 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     3301 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     3361 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     3421 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     3481 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     3541 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     3601 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     3661 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     3721 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     3781 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     3841 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     3901 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
     3961 agacagagac agatccattc gattagtgaa cggatctcga cggtatcggt taacttttaa
     4021 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     4081 agacatacaa actaaagaat tacaaaaaca aattacaaaa ttcaaaattt tatcgtccag
     4141 tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa aagcaggctc caccatggga
     4201 accgacattg attattgact aggcttttgc aaaaagcttt gcaaagatgg ataaagtttt
     4261 aaacagagag gaatctttgc agctaatgga ccttctaggt cttgaaagga gtgggaattg
     4321 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
     4381 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
     4441 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
     4501 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
     4561 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
     4621 cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg
     4681 agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc
     4741 ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt
     4801 tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc
     4861 aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg
     4921 cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag
     4981 cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg
     5041 gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag
     5101 ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatggagga
     5161 cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt
     5221 cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt
     5281 agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg
     5341 agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat
     5401 tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag
     5461 tggttcaaag tttttttctt ccatttcagg tgtcgtgagg aattagcttg gtactaatac
     5521 gactcactat agggagaccc aagctggcta ggtaagcttg gtaccgagct cggatccatg
     5581 tacccatacg atgttccaga ttacgctggt ggatctggag gttctggtgg aatggaatcc
     5641 cttctgcagc acctggatcg cttttccgag cttctggcgg tctcaagcac tacctacgtc
     5701 agcacctggg accccgccac cgtgcgccgg gccttgcagt gggcgcgcta cctgcgccac
     5761 atccatcggc gctttggtcg gcatggcccc attcgcacgg ctctggagcg gcggctgcac
     5821 aaccagtgga ggcaagaggg cggctttggg cggggtccag ttccgggatt agcgaacttc
     5881 caggccctcg gtcactgtga cgtcctgctc tctctgcgcc tgctggagaa ccgggccctc
     5941 ggggatgcag ctcgttacca cctggtgcag caactctttc ccggcccggg cgtccgggac
     6001 gccgatgagg agacactcca agagagcctg gcccgccttg cccgccggcg gtctgcggtg
     6061 cacatgctgc gcttcaatgg ctatagagag aacccaaatc tccaggagga ctctctgatg
     6121 aagacccagg cggagctgct gctggagcgt ctgcaggagg tggggaaggc cgaagcggag
     6181 cgtcccgcca ggtttctcag cagcctgtgg gagcgcttgc ctcagaacaa cttcctgaag
     6241 gtgatagcgg tggcgctgtt gcagccgcct ttgtctcgtc ggccccaaga agagttggaa
     6301 cccggcatcc acaaatcacc tggagagggg agccaagtgc tagtccactg gcttctgggg
     6361 aattcggaag tctttgctgc cttttgtcgc gccctcccag ccgggctttt gactttagtg
     6421 actagccgcc acccagcgct gtctcctgtc tatctgggtc tgctaacaga ctggggtcaa
     6481 cgtttgcact atgaccttca gaaaggcatt tgggttggaa ctgagtccca agatgtgccc
     6541 tgggaggagt tgcacaatag gtttcaaagc ctctgtcagg cccctccacc tctgaaagat
     6601 aaagttctaa ctgccctgga gacctgtaaa gcgcaggatg gagattttga agtacctggt
     6661 cttagcatct ggacagacct cttattagct cttcgtagtg gtgcatttag gaaaagacaa
     6721 gttttgggtc tcagcgcagg cctcagttct gtataggtcg accccctctc cctccccccc
     6781 ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt
     6841 attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt
     6901 cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa
     6961 tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac
     7021 cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg
     7081 tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt
     7141 tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca
     7201 gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt
     7261 ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg
     7321 aaaaacacga tgccaccatg ggtatgaacc cagccatcag cgtcgctctc ctgctctcag
     7381 tcttgcaggt gtcccgaggg cagaaggtga ccagcctgac agcctgcctg gtgaaccaaa
     7441 accttcgcct ggactgccgc catgagaata acaccaagga taactccatc cagcatgagt
     7501 tcagcctgac ccgagagaag aggaagcacg tgctctcagg cacccttggg atacccgagc
     7561 acacgtaccg ctcccgcgtc accctctcca accagcccta tatcaaggtc cttaccctag
     7621 ccaacttcac caccaaggat gagggcgact acttttgtga gcttcgcgtc tcgggcgcga
     7681 atcccatgag ctccaataaa agtatcagtg tgtatagaga caagctggtc aagtgtggcg
     7741 gcataagcct gctggttcag aacacatcct ggatgctgct gctgctgctt tccctctccc
     7801 tcctccaagc cctggacttc atttctctgt gatctagacc cagctttctt gtacaaagtg
     7861 gttgatatcc agcacagtgg cggccgctcg acaatcaacc tctggattac aaaatttgtg
     7921 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     7981 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     8041 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     8101 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     8161 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     8221 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     8281 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg
     8341 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     8401 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     8461 ccctttgggc cgcctccccg cctggacctg caggattctg cagatatccg gactagtgat
     8521 ctaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc
     8581 agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc
     8641 caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc
     8701 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat
     8761 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg
     8821 ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag
     8881 gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc
     8941 ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct
     9001 gttctcctct tcctcatctc cgggcctttc gacctgcagc caatatggga tcggccattg
     9061 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     9121 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     9181 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
     9241 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     9301 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     9361 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     9421 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     9481 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     9541 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg
     9601 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     9661 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     9721 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     9781 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     9841 tcttctgaac gcgtcgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac
     9901 cccatgcatc tcgagggccc ggtaccttta agaccaatga cttacaaggc agctgtagat
     9961 cttagccact ttttaaaaga aaagggggga ctggaagggc tagctcactc ccaacgaaga
    10021 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
    10081 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
    10141 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
    10201 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
    10261 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
    10321 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
    10381 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc
    10441 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
    10501 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
    10561 tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag
    10621 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
    10681 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
    10741 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga
    10801 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
    10861 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
    10921 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
    10981 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
    11041 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
    11101 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
    11161 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
    11221 cgcgaatttt aacaaaatat taacgcttac aatttaggtg
//
