LOCUS       sequence-211960-       10729 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses UBE2T..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10729)
  AUTHORS   Richardson CD, Kazane KR, Feng SJ, Zelin E, Bray NL, Schafer AJ, 
            Floor SN, Corn JE
  TITLE     CRISPR-Cas9 genome editing in human cells occurs via the Fanconi 
            anemia pathway.
  JOURNAL   Nat Genet. 2018 Aug;50(8):1132-1139. doi: 10.1038/s41588-018-0174-0.
            Epub 2018 Jul 27.
  PUBMED    30054595
REFERENCE   2  (bases 1 to 10729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41588-018-0174-0"; journalName: "Nat Genet"; date: 
            "2018-08"; volume: "50"; issue: "8"; pages: "1132-1139"
COMMENT     Sequence Label: pCR1326
FEATURES             Location/Qualifiers
     source          1..10729
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        16..120
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(121..189,190..981)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(339..358)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1152..1740
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1641..1660
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1894..1911
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2028..2049
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2064..2069,2070..2087,2088..2094)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2102..2118
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2107..2129
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2126..2142
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2126..2142
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2161..2181
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2163..2181
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2209..2435
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2436..2616
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2663..2788
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3281..3514
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3699..3743
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4010..4126
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4160..4184
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4315..5493
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4546..5484
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5441..5461
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        5510..5528
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5576..5602
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(6394..6411)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6621..6640
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      6796..6805
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6808..7296
                     /codon_start=1
                     /product="mouse GPI-anchored cell surface glycoprotein of 
                     the immunoglobulin superfamily"
                     /label=Thy1.1
                     /note="This allele of Thy1 is also known as CD90.1."
                     /translation="MNPAISVALLLSVLQVSRGQKVTSLTACLVNQNLRLDCRHENNTK
                     DNSIQHEFSLTREKRKHVLSGTLGIPEHTYRSRVTLSNQPYIKVLTLANFTTKDEGDYF
                     CELRVSGANPMSSNKSISVYRDKLVKCGGISLLVQNTSWMLLLLLSLSLLQALDFISL"
     protein_bind    complement(7302..7326)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    7357..7945
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7410..7430)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7828..7839)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7996..8495
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8031..8054)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8428..8447
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8509..9312
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8572..8591)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9182..9201
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             9456..9689
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9761..9882
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9798..9817)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9852..9871
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9922..10057
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(10078..10096)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10106..10123)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10106..10122)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10115..10137)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10264..10719
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10351..10370)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10561..10582
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
       61 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
      121 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
      181 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
      241 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
      301 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
      361 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
      421 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
      481 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
      541 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
      601 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
      661 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
      721 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
      781 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
      841 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
      901 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
      961 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     1021 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     1081 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     1141 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     1201 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     1261 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     1321 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     1381 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     1441 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     1501 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     1561 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     1621 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     1681 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     1741 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     1801 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     1861 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     1921 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     1981 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     2041 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     2101 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
     2161 gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg
     2221 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
     2281 agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag
     2341 gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag
     2401 atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct
     2461 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     2521 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     2581 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     2641 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     2701 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     2761 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     2821 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     2881 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     2941 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     3001 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     3061 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     3121 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     3181 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     3241 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     3301 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     3361 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     3421 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     3481 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     3541 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     3601 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     3661 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     3721 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     3781 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     3841 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     3901 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     3961 gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact tttaaaagaa
     4021 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     4081 tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg tccagtgtgg
     4141 tggaattctg cagatatcaa caagtttgta caaaaaagca ggctccacca tgggaaccga
     4201 cattgattat tgactaggct tttgcaaaaa gctttgcaaa gatggataaa gttttaaaca
     4261 gagaggaatc tttgcagcta atggaccttc taggtcttga aaggagtggg aattggctcc
     4321 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
     4381 gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
     4441 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
     4501 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt
     4561 ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc
     4621 acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt
     4681 tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg
     4741 cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat
     4801 aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat
     4861 agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc
     4921 ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg
     4981 ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc
     5041 gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg
     5101 tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg
     5161 cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca
     5221 gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc
     5281 tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt
     5341 ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc
     5401 ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt
     5461 caaagttttt ttcttccatt tcaggtgtcg tgaggaatta gcttggtact aatacgactc
     5521 actataggga gacccaagct ggctaggtaa gcttggtacc gagctcggat ccatgtaccc
     5581 atacgatgtt ccagattacg ctggtggatc tggaggttct ggtggaatgc agagagcttc
     5641 acgtctgaag agagagctgc acatgttagc cacagagcca cccccaggca tcacatgttg
     5701 gcaagataaa gaccaaatgg atgacctgcg agctcaaata ttaggtggag ccaacacacc
     5761 ttatgagaaa ggtgttttta agctagaagt tatcattcct gagaggtacc catttgaacc
     5821 tcctcagatc cgatttctca ctccaattta tcatccaaac attgattctg ctggaaggat
     5881 ttgtctggat gttctcaaat tgccaccaaa aggtgcttgg agaccatccc tcaacatcgc
     5941 aactgtgttg acctctattc agctgctcat gtcagaaccc aaccctgatg acccgctcat
     6001 ggctgacata tcctcagaat ttaaatataa taagccagcc ttcctcaaga atgccagaca
     6061 gtggacagag aagcatgcaa gacagaaaca aaaggctgat gaggaagaga tgcttgataa
     6121 tctaccagag gctggtgact ccagagtaca caactcaaca cagaaaagga aggccagtca
     6181 gctagtaggc atagaaaaga aatttcatcc tgatgtttag gtcgaccccc tctccctccc
     6241 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata
     6301 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg
     6361 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt
     6421 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag
     6481 cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc
     6541 cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga
     6601 tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg
     6661 cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat
     6721 gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc
     6781 tttgaaaaac acgatgccac catgggtatg aacccagcca tcagcgtcgc tctcctgctc
     6841 tcagtcttgc aggtgtcccg agggcagaag gtgaccagcc tgacagcctg cctggtgaac
     6901 caaaaccttc gcctggactg ccgccatgag aataacacca aggataactc catccagcat
     6961 gagttcagcc tgacccgaga gaagaggaag cacgtgctct caggcaccct tgggataccc
     7021 gagcacacgt accgctcccg cgtcaccctc tccaaccagc cctatatcaa ggtccttacc
     7081 ctagccaact tcaccaccaa ggatgagggc gactactttt gtgagcttcg cgtctcgggc
     7141 gcgaatccca tgagctccaa taaaagtatc agtgtgtata gagacaagct ggtcaagtgt
     7201 ggcggcataa gcctgctggt tcagaacaca tcctggatgc tgctgctgct gctttccctc
     7261 tccctcctcc aagccctgga cttcatttct ctgtgatcta gacccagctt tcttgtacaa
     7321 agtggttgat atccagcaca gtggcggccg ctcgacaatc aacctctgga ttacaaaatt
     7381 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     7441 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     7501 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     7561 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     7621 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     7681 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     7741 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
     7801 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     7861 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     7921 atctcccttt gggccgcctc cccgcctgga cctgcaggat tctgcagata tccggactag
     7981 tgatctaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt
     8041 tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca
     8101 catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta
     8161 ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac
     8221 aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga
     8281 agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc
     8341 agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg
     8401 gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg
     8461 cgctgttctc ctcttcctca tctccgggcc tttcgacctg cagccaatat gggatcggcc
     8521 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     8581 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     8641 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
     8701 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     8761 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     8821 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     8881 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     8941 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     9001 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
     9061 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     9121 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     9181 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     9241 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     9301 gagttcttct gaacgcgtcg cccgccccac gacccgcagc gcccgaccga aaggagcgca
     9361 cgaccccatg catctcgagg gcccggtacc tttaagacca atgacttaca aggcagctgt
     9421 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctagctc actcccaacg
     9481 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     9541 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     9601 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     9661 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     9721 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     9781 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     9841 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     9901 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     9961 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
    10021 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct
    10081 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
    10141 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
    10201 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
    10261 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
    10321 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
    10381 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
    10441 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
    10501 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
    10561 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
    10621 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
    10681 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcact
//
