LOCUS       sequence-211962-       12501 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses USP1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12501)
  AUTHORS   Richardson CD, Kazane KR, Feng SJ, Zelin E, Bray NL, Schafer AJ, 
            Floor SN, Corn JE
  TITLE     CRISPR-Cas9 genome editing in human cells occurs via the Fanconi 
            anemia pathway.
  JOURNAL   Nat Genet. 2018 Aug;50(8):1132-1139. doi: 10.1038/s41588-018-0174-0.
            Epub 2018 Jul 27.
  PUBMED    30054595
REFERENCE   2  (bases 1 to 12501)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41588-018-0174-0"; journalName: "Nat Genet"; date: 
            "2018-08"; volume: "50"; issue: "8"; pages: "1132-1139"
COMMENT     Sequence Label: pCR1325
FEATURES             Location/Qualifiers
     source          1..12501
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        12..116
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(117..185,186..977)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(335..354)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1148..1736
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1637..1656
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1890..1907
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2024..2045
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2060..2065,2066..2083,2084..2090)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2098..2114
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2103..2125
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2122..2138
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2122..2138
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2157..2177
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2159..2177
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2205..2431
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2432..2612
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2659..2784
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3277..3510
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3695..3739
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4006..4122
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4156..4180
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4311..5489
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4542..5480
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5437..5457
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        5506..5524
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5572..5598
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(8162..8179)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8389..8408
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      8564..8573
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8576..9064
                     /codon_start=1
                     /product="mouse GPI-anchored cell surface glycoprotein of 
                     the immunoglobulin superfamily"
                     /label=Thy1.1
                     /note="This allele of Thy1 is also known as CD90.1."
                     /translation="MNPAISVALLLSVLQVSRGQKVTSLTACLVNQNLRLDCRHENNTK
                     DNSIQHEFSLTREKRKHVLSGTLGIPEHTYRSRVTLSNQPYIKVLTLANFTTKDEGDYF
                     CELRVSGANPMSSNKSISVYRDKLVKCGGISLLVQNTSWMLLLLLSLSLLQALDFISL"
     protein_bind    complement(9070..9094)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    9125..9713
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9178..9198)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9596..9607)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        9764..10263
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9799..9822)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     10196..10215
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             10277..11080
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10340..10359)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10950..10969
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             11224..11457
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    11529..11650
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11566..11585)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11620..11639
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      11690..11825
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(11846..11864)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11874..11891)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11874..11890)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11883..11905)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      12032..12487
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12119..12138)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12329..12350
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
       61 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
      121 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
      181 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
      241 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
      301 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
      361 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
      421 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
      481 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
      541 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
      601 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
      661 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
      721 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
      781 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
      841 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
      901 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
      961 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     1021 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     1081 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     1141 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     1201 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1261 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     1321 accacttcaa gaactctgta gcaccgccta catacctctc tctgctaatc ctgttaccag
     1381 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     1441 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     1501 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     1561 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     1621 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     1681 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     1741 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     1801 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     1861 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     1921 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     1981 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     2041 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     2101 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
     2161 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat
     2221 actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa
     2281 aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag
     2341 gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat
     2401 tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc
     2461 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     2521 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     2581 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     2641 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     2701 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     2761 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     2821 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     2881 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     2941 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     3001 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     3061 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     3121 gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     3181 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     3241 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     3301 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     3361 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     3421 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     3481 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     3541 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     3601 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     3661 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     3721 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     3781 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     3841 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     3901 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
     3961 cagatccatt cgattagtga acggatctcg acggtatcgg ttaactttta aaagaaaagg
     4021 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     4081 aactaaagaa ttacaaaaac aaattacaaa attcaaaatt ttatcgtcca gtgtggtgga
     4141 attctgcaga tatcaacaag tttgtacaaa aaagcaggct ccaccatggg aaccgacatt
     4201 gattattgac taggcttttg caaaaagctt tgcaaagatg gataaagttt taaacagaga
     4261 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg
     4321 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
     4381 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
     4441 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
     4501 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt
     4561 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct
     4621 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga
     4681 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct
     4741 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt
     4801 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc
     4861 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg
     4921 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac
     4981 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc
     5041 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag
     5101 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct
     5161 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg
     5221 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga
     5281 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc
     5341 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg
     5401 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa
     5461 gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata cgactcacta
     5521 tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccat gtacccatac
     5581 gatgttccag attacgctgg tggatctgga ggttctggtg gaatgcctgg tgtcatacct
     5641 agtgaaagta atggactttc aagaggtagc ccttcaaaga aaaacagact ttccttaaag
     5701 ttttttcaga aaaaggaaac taagagagct ttggatttca cagattctca agaaaatgaa
     5761 gaaaaagctt ctgaatatag agcatctgaa attgatcaag ttgttcctgc agcacagtct
     5821 tcacctataa actgtgagaa gagagaaaac ttgttaccat ttgtgggact gaataatctc
     5881 ggcaatactt gctatcttaa tagtatactt caggtattat atttttgtcc cggttttaaa
     5941 tctggagtaa agcacttatt taatattatt tcaaggaaga aagaagctct aaaggatgaa
     6001 gccaatcaaa aagacaaggg aaattgcaaa gaagattctt tggcaagtta tgaattgata
     6061 tgcagtttac agtccttaat catttcggtt gaacagctcc aggctagttt tctcttaaat
     6121 ccagagaaat atactgatga acttgccact cagccaaggc gactgcttaa cacactgagg
     6181 gaactcaacc ctatgtatga aggatatcta cagcatgatg cacaggaagt attacaatgt
     6241 attttgggaa acattcaaga aacatgccaa ctcctaaaaa aagaagaagt aaaaaatgtg
     6301 gcagaattac ctactaaggt agaagaaata cctcatccga aagaggaaat gaatggtatt
     6361 aacagcatag agatggacag tatgaggcat tctgaagact ttaaagagaa actcccaaaa
     6421 ggaaatggga aaagaaaaag tgacactgaa tttggtaaca tgaagaaaaa agttaaatta
     6481 tccaaggaac accagtcatt ggaagagaac cagagacaaa ctagatcaaa aagaaaagct
     6541 acaagtgata cattagagag tcctcctaaa ataattccca agtatatttc tgaaaatgag
     6601 agtccaagac cctcacaaaa gaaatcaaga gttaaaataa attggttaaa gtctgcaact
     6661 aagcaaccca gcattctttc taaattttgt agtctgggaa aaataacaac aaaccaagga
     6721 gtcaaaggac aatctaaaga aaatgaatgt gatcctgaag aggacttggg gaagtgtgaa
     6781 agtgataaca caactaatgg ttgtggactt gaatctccag gaaatactgt tacacctgta
     6841 aatgttaatg aagttaaacc cataaacaaa ggtgaagaac aaattggttt tgagctagtg
     6901 gagaaattat ttcaaggtca gctggtatta aggacgcgtt gcttggaatg tgaaagttta
     6961 acagaaagaa gagaagattt tcaagacatc agtgtgccag tacaagaaga tgagctttcc
     7021 aaagtagagg agagttctga aatttctcca gagccaaaaa cagaaatgaa gaccctgaga
     7081 tgggcaattt cacaatttgc ttcagtagaa aggattgtag gagaagataa atatttctgt
     7141 gaaaactgcc atcattatac tgaagctgaa cgaagtcttt tgtttgacaa aatgcctgaa
     7201 gttataacta ttcatttgaa gtgctttgct gctagtggtt tggagtttga ttgttatggt
     7261 ggtggacttt ccaagatcaa cactccttta ttgacacctc ttaaattgtc actagaagaa
     7321 tggagcacaa agccaactaa cgacagctat ggattatttg cggttgtgat gcatagtggc
     7381 attacaatta gtagtgggca ttacactgct tctgttaaag tcactgacct taacagttta
     7441 gaactagata aaggaaattt tgtggttgac caaatgtgtg aaataggtaa gccagaacca
     7501 ttgaatgagg aggaagcaag gggtgtggtt gagaattata atgatgaaga agtgtcaatt
     7561 agagttggtg gaaatacaca gccaagtaaa gttttgaaca aaaaaaatgt agaagctatt
     7621 ggacttcttg gaggacaaaa gagcaaagca gattatgagc tatacaacaa agcctctaat
     7681 cctgataagg ttgctagtac agcgtttgct gaaaatagaa attctgagac tagtgatact
     7741 actgggaccc atgaatctga tagaaacaag gaatccagtg accaaacagg cattaatatt
     7801 agtggatttg agaacaaaat ttcatacgta gtgcaaagct taaaggagta tgaggggaag
     7861 tggttgcttt ttgatgattc tgaagtcaaa gttactgaag agaaggactt tctgaattct
     7921 ctttcccctt ctacatctcc tacttctact ccttacttgc tattttataa gaaattataa
     7981 gtcgaccccc tctccccctc tccctccccc ccccctaacg ttactggccg aagccgcttg
     8041 gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc
     8101 aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc
     8161 cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa
     8221 gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct
     8281 ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca
     8341 caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca
     8401 agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat
     8461 ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc
     8521 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgccacca tgggtatgaa
     8581 cccagccatc agcgtcgctc tcctgctctc agtcttgcag gtgtcccgag ggcagaaggt
     8641 gaccagcctg acagcctgcc tggtgaacca aaaccttcgc ctggactgcc gccatgagaa
     8701 taacaccaag gataactcca tccagcatga gttcagcctg acccgagaga agaggaagca
     8761 cgtgctctca ggcacccttg ggatacccga gcacacgtac cgctcccgcg tcaccctctc
     8821 caaccagccc tatatcaagg tccttaccct agccaacttc accaccaagg atgagggcga
     8881 ctacttttgt gagcttcgcg tctcgggcgc gaatcccatg agctccaata aaagtatcag
     8941 tgtgtataga gacaagctgg tcaagtgtgg cggcataagc ctgctggttc agaacacatc
     9001 ctggatgctg ctgctgctgc tttccctctc cctcctccaa gccctggact tcatttctct
     9061 gtgatctaga cccagctttc ttgtacaaag tggttgatat ccagcacagt ggcggccgct
     9121 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     9181 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     9241 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     9301 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     9361 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     9421 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     9481 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct
     9541 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     9601 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     9661 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggacc
     9721 tgcaggattc tgcagatatc cggactagtg atctaattct accgggtagg ggaggcgctt
     9781 ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca
     9841 agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc
     9901 tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc
     9961 ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg
    10021 cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag
    10081 cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg
    10141 ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc
    10201 tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt
    10261 tcgacctgca gccaatatgg gatcggccat tgaacaagat ggattgcacg caggttctcc
    10321 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
    10381 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
    10441 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
    10501 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
    10561 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
    10621 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
    10681 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
    10741 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
    10801 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
    10861 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
    10921 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
    10981 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
    11041 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga acgcgtcgcc cgccccacga
    11101 cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca tctcgagggc ccggtacctt
    11161 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
    11221 gactggaagg gctagctcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
    11281 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
    11341 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
    11401 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
    11461 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
    11521 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
    11581 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
    11641 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
    11701 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
    11761 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
    11821 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
    11881 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
    11941 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
    12001 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
    12061 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
    12121 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
    12181 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
    12241 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
    12301 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
    12361 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
    12421 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
    12481 acaatttagg tggcactttt c
//
