LOCUS       sequence-211965-        7875 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PsopB driven sopB and sigE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7875)
  AUTHORS   Knodler LA, Winfree S, Drecktrah D, Ireland R, Steele-Mortimer O
  TITLE     Ubiquitination of the bacterial inositol phosphatase, SopB, 
            regulates its biological activity at the plasma membrane.
  JOURNAL   Cell Microbiol. 2009 Nov;11(11):1652-70. doi: 
            10.1111/j.1462-5822.2009.01356.x. Epub 2009 Jul 13.
  PUBMED    19614667
REFERENCE   2  (bases 1 to 7875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1462-5822.2009.01356.x"; journalName: "Cell Microbiol"; 
            date: "2009-11"; volume: "11"; issue: "11"; pages: "1652-70"
COMMENT     Sequence Label: pSopBWT
FEATURES             Location/Qualifiers
     source          1..7875
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(144..935,936..1004))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     767..786
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1005..1109)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1135..1590)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1272..1293)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1484..1503
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1717..1739
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1731..1748
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1732..1748
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1758..1777
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1758..1776
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     1801..1817
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1802..1818
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4346..4366)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4346..4364)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4385..4401)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4385..4401)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4398..4420)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4409..4425
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4433..4439,4440..4457,4458..4463))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4478..4499
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4616..4633)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      5778..6000
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             6048..6998
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
ORIGIN
        1 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
       61 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
      121 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
      181 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
      241 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
      301 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
      361 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
      421 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
      481 tcgtcgtttg gtatggcttc attcagctcc ggttcccagc gatcaaggcg agttacatga
      541 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
      601 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
      661 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
      721 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
      781 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
      841 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
      901 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
      961 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     1021 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     1081 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctaaattg
     1141 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     1201 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     1261 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     1321 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     1381 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     1441 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     1501 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     1561 ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg cgcaactgtt
     1621 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
     1681 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
     1741 cggccagtga gcgcgcgtaa tacgactcac tatagggcga attgggtacc gggccccccc
     1801 tcgaggtcga cggtatcgat aagctttctg ttcaagcatg gaataggaaa aacgaatatt
     1861 cttcgtcacg gtcttacttg tccggggctt tgctggcata cacacacctg tataacattt
     1921 gatgtaacgc cgttacttta cgcaggagta aatcggtgaa tttgatctga gtcaagaagg
     1981 tgggttttca ataaaagttg tgccataaat tgtgaagttt gtagatttta tgaacatttg
     2041 atgtaccgat ctcccccatg atcgccacta cgtatggacg tcaggatgcc tccccgcctg
     2101 atcagaagcg tttcctcatt aaaaaggaca tttttttaaa gttcctggtg cataaaagtc
     2161 acatcctttt aaagggttgt taaccctgtt gaatgttccc actcccctat tcaggaatat
     2221 taaaaacgct atgcaaatac agagcttcta tcactcagct tcactaaaaa cccaggaggc
     2281 ttttaaaagc ctacaaaaaa ccttatacaa cggaatgcag attctctcag gccagggcaa
     2341 agcgccggct aaagcgcccg acgctcgccc ggaaattatt gtcctgcgag aacccggcgc
     2401 gacatggggg aattatctac agcatcagaa ggcgtctaac cactcgctgc ataacctcta
     2461 taacttacag cgcgatcttc ttaccgtcgc ggcaaccgtt ctgggtaaac aagacccggt
     2521 tctaacgtca atggcaaacc aaatggagtt agccaaagtt aaagcggacc ggccagcaac
     2581 aaaacaagaa gaagccgcgg caaaagcatt gaagaaaaat cttatcgaac ttattgcagc
     2641 acgcactcag cagcaggatg gcttacctgc aaaagaagct catcgctttg cggcagtagc
     2701 gtttagagat gctcaggtca agcagcttaa taaccagccc tggcaaacca taaaaaatac
     2761 actcacgcat aacgggcatc actataccaa cacgcagctc cctgcagcag agatgaaaat
     2821 cggcgcaaaa gatatctttc ccagtgctta tgagggaaag ggcgtatgca gttgggatac
     2881 caagaatatt catcacgcca ataatttgtg gatgtccacg gtgagtgtgc atgaggacgg
     2941 taaagataaa acgctttttt gcgggatacg tcatggcgtg ctttccccct atcatgaaaa
     3001 agatccgctt ctgcgtcacg tcggcgctga aaacaaagcc aaagaagtat taactgcggc
     3061 actttttagt aaacctgagt tgcttaacaa agccttagcg ggcgaggcag taagcctgaa
     3121 actggtatcc gtcgggttac tcaccgcgtc gaatattttc ggcaaagagg gaacgatggt
     3181 cgaggaccaa atgcgcgcat ggcaatcgtt gacccagccg ggaaaaatga ttcatttaaa
     3241 aatccgcaat aaagatggcg atctacagac ggtaaaaata aaaccggacg tcgccgcatt
     3301 taatgtgggt gttaatgagc tggcgctcaa gctcggcttt ggccttaagg catcggatag
     3361 ctataatgcc gaggcgctac atcagttatt aggcaatgat ttacgccctg aagccagacc
     3421 aggtggctgg gttggcgaat ggctggcgca atacccggat aattatgagg tcgtcaatac
     3481 attagcgcgc cagattaagg atatatggaa aaataaccaa catcataaag atggcggcga
     3541 accctataaa ctcgcacaac gccttgccat gttagcccat gaaattgacg cggtacccgc
     3601 ctggaattgt aaaagcggca aagatcgtac agggatgatg gattcagaaa tcaagcgaga
     3661 gatcatttcc ttacatcaga cccatatgtt aagtgcgcct ggtagtcttc cggatagcgg
     3721 tggacagaaa attttccaaa aagtattact gaatagcggt aacctggaga ttcagaagca
     3781 aaatacgggc ggggcgggaa acaaagtaat gaaaaattta tcgccagagg tgctcaatct
     3841 ttcctatcaa aaacgagttg gggatgaaaa tatttggcag tcagtaaaag gcatttcttc
     3901 attaatcaca tcttgagtct tgaggtaact atatggaaag tctattaaat cgtttatatg
     3961 acgcgttagg cctggatgcg ccagaagatg agccactgct tatcattgat gatgggatac
     4021 aggtttattt taatgaatcc gatcatacac tggaaatgtg ctgtcccttt atgccattgc
     4081 ctgacgacat cctgactttg cagcattttt tacgtctgaa ctacaccagc gccgtcacta
     4141 tcggcgctga cgcagacaat actgctttag tggcgcttta tcgcttgccg caaaccagta
     4201 ccgaagaaga ggcgctcact ggttttgaat tattcatttc aaacgtgaag caattgaaag
     4261 agcattatgc ataatttaat acgtcaacat actttcttaa tgagctagag cggccgccac
     4321 cgcggtggag ctccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat
     4381 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     4441 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     4501 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     4561 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     4621 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     4681 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagatctct
     4741 acgggtcgga tttgaagtcg tcttggtagg aggcagcctg aatggcgaat gccgatgccc
     4801 ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc
     4861 gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac agggtgccgg cagcgctctg
     4921 ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc tgtcgcttgc
     4981 ggtattcgga atcttgcacg ccctcgctca agccttcgtc actggtcccg ccaccaaacg
     5041 tttcggcgag aagcaggcca ttatcgccgg catggcggcc gacgcgctgg gctacgtctt
     5101 gctggcgttc gcgacgcgag gctggatggc cttccccatt atgattcttc tcgcttccgg
     5161 cggcatcggg atgcccgcgt tgcaggccat gctgtccagg caggtagatg acgaccatca
     5221 gggacagctt caaggatcgc tcgcggctct taccagccta acttcgatca ttggaccgct
     5281 gatcgtcacg gcgatttatg ccgcctcggc gagcacatgg aacgggttgg catggattgt
     5341 aggcgccgcc ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat ggagccgggc
     5401 cacctcgacc tgaatggaag ccggcggcac ctcgctaacg gattcaccac tccgcagacc
     5461 cgccataaaa cgccctgaga agcccgtgac gggcttttct tgtattatgg gtagtttcct
     5521 tgcatgaatc cataaaaggc gcctgtagtg ccatttaccc ccattcactg ccagagccgt
     5581 gagcgcagcg aactgaatgt cacgaaaaag acagcgactc aggtgcctga tggtcggaga
     5641 caaaaggaat attcagcgat ttgcccgagc ttgcgagggt gctacttaag cctttagggt
     5701 tttaaggtct gttttgtaga ggagcaaaca gcgtttgcga catccttttg taatactgcg
     5761 gaactgacta aagtagtgag ttatacacag ggctgggatc tattcttttt atcttttttt
     5821 attctttctt tattctataa attataacca cttgaatata aacaaaaaaa acacacaaag
     5881 gtctagcgga atttacagag ggtctagcag aatttacaag ttttccagca aaggtctagc
     5941 agaatttaca gatacccaca actcaaagga aaaggactag taattatcat tgactagccc
     6001 atctcaattg gtatagtgat taaaatcacc tagaccaatt gagatgtatg tctgaattag
     6061 ttgttttcaa agcaaatgaa ctagcgatta gtcgctatga cttaacggag catgaaacca
     6121 agctaatttt atgctgtgtg gcactactca accccacgat tgaaaaccct acaaggaaag
     6181 aacggacggt atcgttcact tataaccaat acgctcagat gatgaacatc agtagggaaa
     6241 atgcttatgg tgtattagct aaagcaacca gagagctgat gacgagaact gtggaaatca
     6301 ggaatccttt ggttaaaggc tttgagattt tccagtggac aaactatgcc aagttctcaa
     6361 gcgaaaaatt agaattagtt tttagtgaag agatattgcc ttatcttttc cagttaaaaa
     6421 aattcataaa atataatctg gaacatgtta agtcttttga aaacaaatac tctatgagga
     6481 tttatgagtg gttattaaaa gaactaacac aaaagaaaac tcacaaggca aatatagaga
     6541 ttagccttga tgaatttaag ttcatgttaa tgcttgaaaa taactaccat gagtttaaaa
     6601 ggcttaacca atgggttttg aaaccaataa gtaaagattt aaacacttac agcaatatga
     6661 aattggtggt tgataagcga ggccgcccga ctgatacgtt gattttccaa gttgaactag
     6721 atagacaaat ggatctcgta accgaacttg agaacaacca gataaaaatg aatggtgaca
     6781 aaataccaac aaccattaca tcagattcct acctacataa cggactaaga aaaacactac
     6841 acgatgcttt aactgcaaaa attcagctca ccagttttga ggcaaaattt ttgagtgaca
     6901 tgcaaagtaa gtatgatctc aatggttcgt tctcatggct cacgcaaaaa caacgaacca
     6961 cactagagaa catactggct aaatacggaa ggatctgagg ttcttatggc tcttgtatct
     7021 atcagtgaag catcaagact aacaaacaaa agtagaacaa ctgttcaccg ttacatatca
     7081 aagggaaaac tgtccatatg cacagatgaa aacggtgtaa aaaagataga tacatcagag
     7141 cttttacgag tttttggtgc attcaaagct gttcaccatg aacagatcga caatgtaaca
     7201 gatgaacagc atgtaacacc taatagaaca ggtgaaacca gtaaaacaaa gcaactagaa
     7261 catgaaattg aacacctgag acaacttgtt acagctcaac agtcacacat agacagcctg
     7321 aaacaggcga tgctgcttat cgaatcaaag ctgccgacaa cacgggagcc agtgacgcct
     7381 cccgtgggga aaaaatcatg gcaattctgg aagaaatagc gctttcagcc ggcaaaccgg
     7441 ctgaagccgg atctgcgatt ctgataacaa actagcaaca ccagaacagc ccgtttgcgg
     7501 gcagcaaaac ccgtactttt ggacgttccg gcggtttttt gtggcgagtg gtgttcgggc
     7561 ggtgcgcgca agatccatta tgttaaacgg gcgagtttac atctcaaaac cgcccgctta
     7621 acaccatcag aaatcctcag cgcgatttta agcaccaacc cccccccgta acacccaaat
     7681 ccatactgaa agtggctttg ttgaataaat cagatttcgg gtaagtctcc cccgtagcgg
     7741 gttgtgtttt caggcaatac gcacgctttc aggcatacct gctttcgtca ttttgttcag
     7801 cgctcgtacc agggccatag cctccgcaac ctgaccatcg tagtcacgca gcgtcagtga
     7861 acccccgaac agaga
//
