LOCUS       sequence-211966-        7929 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PsopB driven sopB-2xHA and sigE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7929)
  AUTHORS   Knodler LA, Winfree S, Drecktrah D, Ireland R, Steele-Mortimer O
  TITLE     Ubiquitination of the bacterial inositol phosphatase, SopB, 
            regulates its biological activity at the plasma membrane.
  JOURNAL   Cell Microbiol. 2009 Nov;11(11):1652-70. doi: 
            10.1111/j.1462-5822.2009.01356.x. Epub 2009 Jul 13.
  PUBMED    19614667
REFERENCE   2  (bases 1 to 7929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1462-5822.2009.01356.x"; journalName: "Cell Microbiol"; 
            date: "2009-11"; volume: "11"; issue: "11"; pages: "1652-70"
COMMENT     Sequence Label: pSopB2xHA
FEATURES             Location/Qualifiers
     source          1..7929
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(144..935,936..1004))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     767..786
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1005..1109)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1135..1590)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1272..1293)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1484..1503
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1717..1739
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1731..1748
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1732..1748
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1758..1777
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1758..1776
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     1801..1817
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1802..1818
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3914..3940
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3941..3967
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(4400..4420)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4400..4418)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4439..4455)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4439..4455)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4452..4474)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4463..4479
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4487..4493,4494..4511,4512..4517))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4532..4553
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4670..4687)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      5832..6054
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             6102..7052
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
ORIGIN
        1 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
       61 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
      121 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
      181 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
      241 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
      301 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
      361 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
      421 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
      481 tcgtcgtttg gtatggcttc attcagctcc ggttcccagc gatcaaggcg agttacatga
      541 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
      601 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
      661 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
      721 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
      781 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
      841 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
      901 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
      961 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     1021 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     1081 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctaaattg
     1141 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     1201 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     1261 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     1321 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     1381 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     1441 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     1501 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     1561 ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg cgcaactgtt
     1621 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
     1681 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
     1741 cggccagtga gcgcgcgtaa tacgactcac tatagggcga attgggtacc gggccccccc
     1801 tcgaggtcga cggtatcgat aagctttctg ttcaagcatg gaataggaaa aacgaatatt
     1861 cttcgtcacg gtcttacttg tccggggctt tgctggcata cacacacctg tataacattt
     1921 gatgtaacgc cgttacttta cgcaggagta aatcggtgaa tttgatctga gtcaagaagg
     1981 tgggttttca ataaaagttg tgccataaat tgtgaagttt gtagatttta tgaacatttg
     2041 atgtaccgat ctcccccatg atcgccacta cgtatggacg tcaggatgcc tccccgcctg
     2101 atcagaagcg tttcctcatt aaaaaggaca tttttttaaa gttcctggtg cataaaagtc
     2161 acatcctttt aaagggttgt taaccctgtt gaatgttccc actcccctat tcaggaatat
     2221 taaaaacgct atgcaaatac agagcttcta tcactcagct tcactaaaaa cccaggaggc
     2281 ttttaaaagc ctacaaaaaa ccttatacaa cggaatgcag attctctcag gccagggcaa
     2341 agcgccggct aaagcgcccg acgctcgccc ggaaattatt gtcctgcgag aacccggcgc
     2401 gacatggggg aattatctac agcatcagaa ggcgtctaac cactcgctgc ataacctcta
     2461 taacttacag cgcgatcttc ttaccgtcgc ggcaaccgtt ctgggtaaac aagacccggt
     2521 tctaacgtca atggcaaacc aaatggagtt agccaaagtt aaagcggacc ggccagcaac
     2581 aaaacaagaa gaagccgcgg caaaagcatt gaagaaaaat cttatcgaac ttattgcagc
     2641 acgcactcag cagcaggatg gcttacctgc aaaagaagct catcgctttg cggcagtagc
     2701 gtttagagat gctcaggtca agcagcttaa taaccagccc tggcaaacca taaaaaatac
     2761 actcacgcat aacgggcatc actataccaa cacgcagctc cctgcagcag agatgaaaat
     2821 cggcgcaaaa gatatctttc ccagtgctta tgagggaaag ggcgtatgca gttgggatac
     2881 caagaatatt catcacgcca ataatttgtg gatgtccacg gtgagtgtgc atgaggacgg
     2941 taaagataaa acgctttttt gcgggatacg tcatggcgtg ctttccccct atcatgaaaa
     3001 agatccgctt ctgcgtcacg tcggcgctga aaacaaagcc aaagaagtat taactgcggc
     3061 actttttagt aaacctgagt tgcttaacaa agccttagcg ggcgaggcag taagcctgaa
     3121 actggtatcc gtcgggttac tcaccgcgtc gaatattttc ggcaaagagg gaacgatggt
     3181 cgaggaccaa atgcgcgcat ggcaatcgtt gacccagccg ggaaaaatga ttcatttaaa
     3241 aatccgcaat aaagatggcg atctacagac ggtaaaaata aaaccggacg tcgccgcatt
     3301 taatgtgggt gttaatgagc tggcgctcaa gctcggcttt ggccttaagg catcggatag
     3361 ctataatgcc gaggcgctac atcagttatt aggcaatgat ttacgccctg aagccagacc
     3421 aggtggctgg gttggcgaat ggctggcgca atacccggat aattatgagg tcgtcaatac
     3481 attagcgcgc cagattaagg atatatggaa aaataaccaa catcataaag atggcggcga
     3541 accctataaa ctcgcacaac gccttgccat gttagcccat gaaattgacg cggtacccgc
     3601 ctggaattgt aaaagcggca aagatcgtac agggatgatg gattcagaaa tcaagcgaga
     3661 gatcatttcc ttacatcaga cccatatgtt aagtgcgcct ggtagtcttc cggatagcgg
     3721 tggacagaaa attttccaaa aagtattact gaatagcggt aacctggaga ttcagaagca
     3781 aaatacgggc ggggcgggaa acaaagtaat gaaaaattta tcgccagagg tgctcaatct
     3841 ttcctatcaa aaacgagttg gggatgaaaa tatttggcag tcagtaaaag gcatttcttc
     3901 attaatcaca tcttatccgt atgatgtgcc ggattatgcg tatccgtatg atgtgccgga
     3961 ttatgcgtga gtcttgaggt aactatatgg aaagtctatt aaatcgttta tatgacgcgt
     4021 taggcctgga tgcgccagaa gatgagccac tgcttatcat tgatgatggg atacaggttt
     4081 attttaatga atccgatcat acactggaaa tgtgctgtcc ctttatgcca ttgcctgacg
     4141 acatcctgac tttgcagcat tttttacgtc tgaactacac cagcgccgtc actatcggcg
     4201 ctgacgcaga caatactgct ttagtggcgc tttatcgctt gccgcaaacc agtaccgaag
     4261 aagaggcgct cactggtttt gaattattca tttcaaacgt gaagcaattg aaagagcatt
     4321 atgcataatt taatacgtca acatactttc ttaatgagct agagcggccg ccaccgcggt
     4381 ggagctccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt
     4441 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     4501 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     4561 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     4621 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     4681 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     4741 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagat ctctacgggt
     4801 cggatttgaa gtcgtcttgg taggaggcag cctgaatggc gaatgccgat gcccttgaga
     4861 gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt
     4921 atgactgtct tctttatcat gcaactcgta ggacagggtg ccggcagcgc tctgggtcat
     4981 tttcggcgag gaccgctttc gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt
     5041 cggaatcttg cacgccctcg ctcaagcctt cgtcactggt cccgccacca aacgtttcgg
     5101 cgagaagcag gccattatcg ccggcatggc ggccgacgcg ctgggctacg tcttgctggc
     5161 gttcgcgacg cgaggctgga tggccttccc cattatgatt cttctcgctt ccggcggcat
     5221 cgggatgccc gcgttgcagg ccatgctgtc caggcaggta gatgacgacc atcagggaca
     5281 gcttcaagga tcgctcgcgg ctcttaccag cctaacttcg atcattggac cgctgatcgt
     5341 cacggcgatt tatgccgcct cggcgagcac atggaacggg ttggcatgga ttgtaggcgc
     5401 cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt gcatggagcc gggccacctc
     5461 gacctgaatg gaagccggcg gcacctcgct aacggattca ccactccgca gacccgccat
     5521 aaaacgccct gagaagcccg tgacgggctt ttcttgtatt atgggtagtt tccttgcatg
     5581 aatccataaa aggcgcctgt agtgccattt acccccattc actgccagag ccgtgagcgc
     5641 agcgaactga atgtcacgaa aaagacagcg actcaggtgc ctgatggtcg gagacaaaag
     5701 gaatattcag cgatttgccc gagcttgcga gggtgctact taagccttta gggttttaag
     5761 gtctgttttg tagaggagca aacagcgttt gcgacatcct tttgtaatac tgcggaactg
     5821 actaaagtag tgagttatac acagggctgg gatctattct ttttatcttt ttttattctt
     5881 tctttattct ataaattata accacttgaa tataaacaaa aaaaacacac aaaggtctag
     5941 cggaatttac agagggtcta gcagaattta caagttttcc agcaaaggtc tagcagaatt
     6001 tacagatacc cacaactcaa aggaaaagga ctagtaatta tcattgacta gcccatctca
     6061 attggtatag tgattaaaat cacctagacc aattgagatg tatgtctgaa ttagttgttt
     6121 tcaaagcaaa tgaactagcg attagtcgct atgacttaac ggagcatgaa accaagctaa
     6181 ttttatgctg tgtggcacta ctcaacccca cgattgaaaa ccctacaagg aaagaacgga
     6241 cggtatcgtt cacttataac caatacgctc agatgatgaa catcagtagg gaaaatgctt
     6301 atggtgtatt agctaaagca accagagagc tgatgacgag aactgtggaa atcaggaatc
     6361 ctttggttaa aggctttgag attttccagt ggacaaacta tgccaagttc tcaagcgaaa
     6421 aattagaatt agtttttagt gaagagatat tgccttatct tttccagtta aaaaaattca
     6481 taaaatataa tctggaacat gttaagtctt ttgaaaacaa atactctatg aggatttatg
     6541 agtggttatt aaaagaacta acacaaaaga aaactcacaa ggcaaatata gagattagcc
     6601 ttgatgaatt taagttcatg ttaatgcttg aaaataacta ccatgagttt aaaaggctta
     6661 accaatgggt tttgaaacca ataagtaaag atttaaacac ttacagcaat atgaaattgg
     6721 tggttgataa gcgaggccgc ccgactgata cgttgatttt ccaagttgaa ctagatagac
     6781 aaatggatct cgtaaccgaa cttgagaaca accagataaa aatgaatggt gacaaaatac
     6841 caacaaccat tacatcagat tcctacctac ataacggact aagaaaaaca ctacacgatg
     6901 ctttaactgc aaaaattcag ctcaccagtt ttgaggcaaa atttttgagt gacatgcaaa
     6961 gtaagtatga tctcaatggt tcgttctcat ggctcacgca aaaacaacga accacactag
     7021 agaacatact ggctaaatac ggaaggatct gaggttctta tggctcttgt atctatcagt
     7081 gaagcatcaa gactaacaaa caaaagtaga acaactgttc accgttacat atcaaaggga
     7141 aaactgtcca tatgcacaga tgaaaacggt gtaaaaaaga tagatacatc agagctttta
     7201 cgagtttttg gtgcattcaa agctgttcac catgaacaga tcgacaatgt aacagatgaa
     7261 cagcatgtaa cacctaatag aacaggtgaa accagtaaaa caaagcaact agaacatgaa
     7321 attgaacacc tgagacaact tgttacagct caacagtcac acatagacag cctgaaacag
     7381 gcgatgctgc ttatcgaatc aaagctgccg acaacacggg agccagtgac gcctcccgtg
     7441 gggaaaaaat catggcaatt ctggaagaaa tagcgctttc agccggcaaa ccggctgaag
     7501 ccggatctgc gattctgata acaaactagc aacaccagaa cagcccgttt gcgggcagca
     7561 aaacccgtac ttttggacgt tccggcggtt ttttgtggcg agtggtgttc gggcggtgcg
     7621 cgcaagatcc attatgttaa acgggcgagt ttacatctca aaaccgcccg cttaacacca
     7681 tcagaaatcc tcagcgcgat tttaagcacc aacccccccc cgtaacaccc aaatccatac
     7741 tgaaagtggc tttgttgaat aaatcagatt tcgggtaagt ctcccccgta gcgggttgtg
     7801 ttttcaggca atacgcacgc tttcaggcat acctgctttc gtcattttgt tcagcgctcg
     7861 taccagggcc atagcctccg caacctgacc atcgtagtca cgcagcgtca gtgaaccccc
     7921 gaacagaga
//
