LOCUS       sequence-211969-        5090 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ProB driven gfpmut3 expression with synTT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5090)
  AUTHORS   Cooper KG, Chong A, Starr T, Finn CE, Steele-Mortimer O
  TITLE     Predictable, Tunable Protein Production in Salmonella for Studying 
            Host-Pathogen Interactions.
  JOURNAL   Front Cell Infect Microbiol. 2017 Nov 16;7:475. doi: 
            10.3389/fcimb.2017.00475. eCollection 2017.
  PUBMED    29201859
REFERENCE   2  (bases 1 to 5090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Cell 
            Infect Microbiol."; date: "2017-11-16"; volume: "7:475. doi"; pages:
            " 10.3389/fcimb.2017.00475. eCollection 2017"
COMMENT     Sequence Label: pCON1-ProB.gfp
FEATURES             Location/Qualifiers
     source          1..5090
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             217..930
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(247..275)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     870..890
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      965..1036
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1052..1079
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     oriT            1692..1779
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     primer_bind     complement(3092..3109)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3263..3851)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3343..3362)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4022..4813,4814..4882))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4645..4664
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4883..4987)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5055..5073
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ttcgagctcg gtacccgggc acagctaaca ccacgtcgtc cctatctgct gccctaggtc
       61 tatgagtggt tgctggataa ctttacgggc atgcataagg ctcgtaatat atattcaggg
      121 agaccacaac ggtttccctc tacaaataat tttgtttaac ttttactaga gtcacacagg
      181 aaagtactag tctagattta agaaggagat atacatatga gtaaaggaga agaacttttc
      241 actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct
      301 gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc
      361 actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg gtatggtgtt
      421 caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa gagtgccatg
      481 cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca
      541 cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt
      601 gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac
      661 aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt caaaattaga
      721 cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt
      781 ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg
      841 aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg
      901 attacacatg gcatggatga actatacaaa taaatctcct gcaggcatgc aagcttccag
      961 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     1021 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     1081 ttataaagct taatgcggta gtttatcaca gttaaattgc taacgcagtc aggcaccgtg
     1141 tatgaaatct aacaatgcgc tcatcgtcat cctcggcacc gtcaccctgg atgctgtagg
     1201 cataggcttg gttatgccgg tactgccggg cctcttgcgg gatatcgtcc attccgacag
     1261 catcgccagt cactatggcg tgctgctagc gcttttagcc gctttagcgg cctttccccc
     1321 tacccgaagg gtgggggcgc gtgtgcagcc ccgcagggcc tgtctcggtc gatcattcag
     1381 cccggctcat ccttctggcg tggcggcaga ccgaacaagg cgcggtcgtg gtcgcgttca
     1441 aggtacgcat ccattgccgc catgagccga tcctccggcc actcgctgct gttcaccttg
     1501 gccaaaatca tggcccccac cagcaccttg cgccttgttt cgttcttgcg ctcttgctgc
     1561 tgttcccttg cccgcacccg ctgaatttcg gcattgattc gcgctcgttg ttcttcgagc
     1621 ttggccagcc gatccgccgc cttgttgctc cccttaacca tcttgacacc ccattgttaa
     1681 tgtgctgtct cgtaggctat catggaggca cagcggcggc aatcccgacc ctactttgta
     1741 ggggagggcg cacttaccgg tttctcttcg agaaactggc ctaacggcca cccttcgggc
     1801 ggtgcgctct ccgagggcca ttgcatggag ccgaaaagca aaagcaacag cgaggcagca
     1861 tggcgattta tcaccttacg gcgaaaaccg gcagcaggtc gggcggccaa tcggccaggg
     1921 ccaaggccga ctacatccag cgcgaaggca agtatgcccg cgacatggat gaagtcttgc
     1981 acgccgaatc cgggcacatg ccggagttcg tcgagcggcc cgccgactac tgggatgctg
     2041 ccgacctgta tgaacgcgcc aatgggcggc tgttcaagga ggtcgaattt gccctgccgg
     2101 tcgagctgac cctcgaccag cagaaggcgc tggcgtccga gttcgcccag cacctgaccg
     2161 gtgccgagcg cctgccgtat acgctggcca tccatgccgg tggcggcgag aacccgcact
     2221 gccacctgat gatctccgag cggatcaatg acggcatcga gcggcccgcc gctcagtggt
     2281 tcaagcggta caacggcaag accccggaga agggcggggc acagaagacc gaagcgctca
     2341 agcccaaggc atggcttgag cagacccgcg aggcatgggc cgaccatgcc aaccgggcat
     2401 tagagcgggc tggccacgac gcccgcattg accacagaac acttgaggcg cagggcatcg
     2461 agcgcctgcc cggtgttcac ctggggccga acgtggtgga gatggaaggc cggggcatcc
     2521 gcaccgaccg ggcagacgtg gccctgaaca tcgacaccgc caacgcccag atcatcgact
     2581 tacaggaata ccgggaggca atagaccatg aacgcaatcg acagagtgaa gaaatccaga
     2641 ggcatcaacg agttagcgga gcagatcgaa ccgctggccc agagcatggc gacactggcc
     2701 gacgaagccc ggcaggtcat gagccagacc cagcaggcca gcgaggcgca ggcggcggag
     2761 tggctgaaag cccagcgcca gacaggggcg gcatgggtgg agctggccaa agagttgcgg
     2821 gaggtagccg ccgaggtgag cagcgccgcg cagagcgccc ggagcgcgtc gcgggggtgg
     2881 cactggaagc tatggctaac cgtgatgctg gcttccatga tgcctacggt ggtgctgctg
     2941 atcgcatcgt tgctcttgct cgacctgacg ccactgacaa ccgaggacgg ctcgatctgg
     3001 ctgcgcttgg tggcccgatg aagaacgaca ggactttgca ggccataggc cgacagctca
     3061 aggccatggg ctgtgagcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     3121 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     3181 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     3241 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     3301 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     3361 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     3421 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     3481 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     3541 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     3601 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     3661 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     3721 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     3781 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     3841 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     3901 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     3961 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     4021 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     4081 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     4141 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     4201 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     4261 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     4321 acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     4381 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     4441 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     4501 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     4561 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     4621 gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     4681 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     4741 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     4801 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     4861 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     4921 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     4981 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     5041 cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaagaa
//
