LOCUS       sequence-21197-e         717 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Taube R, Lin X, Irwin D, Fujinaga K, Peterlin BM
  TITLE     Interaction between P-TEFb and the C-terminal domain of RNA 
            polymerase II activates transcriptional elongation from sites 
            upstream or downstream of target genes.
  JOURNAL   Mol Cell Biol. 2002 Jan . 22(1):321-31.
  PUBMED    11739744
REFERENCE   2  (bases 1 to 717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2002 Jan . 22(1):321-31."
COMMENT     Sequence Label: pEF myc Gal cyclin T1 (1-726) (P#1026)
FEATURES             Location/Qualifiers
     source          1..717
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             68..85
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             95..124
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             140..580
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
ORIGIN
        1 ttagcttgct tacatttgct tctgacacaa ctgtgttcac tagcaacctc aaacagacac
       61 catgccgcat caccaccatc atcacgccat gccggagcag aagctgatat ccgaggagga
      121 cctggccatg gccggatcca tgaagctact gtcttctatc gaacaagcat gcgatatttg
      181 ccgacttaaa aagctcaagt gctccaaaga aaaaccgaag tgcgccaagt gtctgaagaa
      241 caactgggag tgtcgctact ctcccaaaac caaaaggtct ccgctgacta gggcacatct
      301 gacagaagtg gaatcaaggc tagaaagact ggaacagcta tttctactga tttttcctcg
      361 agaagacctt gacatgattt tgaaaatgga ttctttacag gatataaaag cattgttaac
      421 aggattattt gtacaagata atgtgaataa agatgccgtc acagatagat tggcttcagt
      481 ggagactgat atgcctctaa cattgagaca gcatagaata agtgcgacat catcatcgga
      541 agagagtagt aacaaaggtc aaagacagtt gactgtatcg ccggaattcc ccatggaggg
      601 agagaggaag aacaacaaca aacggtggta tttcactcga gaacagctgg aaaatagccc
      661 atcccgtcgt tttggcgtgg acccagataa agaactttct tatcgccagc aggcggc
//
