LOCUS       sequence-211976-        5507 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PrpsM driven mCherry expression with synTT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5507)
  AUTHORS   Cooper KG, Chong A, Starr T, Finn CE, Steele-Mortimer O
  TITLE     Predictable, Tunable Protein Production in Salmonella for Studying 
            Host-Pathogen Interactions.
  JOURNAL   Front Cell Infect Microbiol. 2017 Nov 16;7:475. doi: 
            10.3389/fcimb.2017.00475. eCollection 2017.
  PUBMED    29201859
REFERENCE   2  (bases 1 to 5507)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Cell 
            Infect Microbiol."; date: "2017-11-16"; volume: "7:475. doi"; pages:
            " 10.3389/fcimb.2017.00475. eCollection 2017"
COMMENT     Sequence Label: pFPV-mCherry-TT
FEATURES             Location/Qualifiers
     source          1..5507
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             651..1361
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(796..814)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1064..1083
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      1382..1453
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1469..1496
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     oriT            2109..2196
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     primer_bind     complement(3509..3526)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3680..4268)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3760..3779)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4439..5230,5231..5299))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5062..5081
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5300..5404)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5472..5490
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ttcgagctcg gtacccgggg atcccggagt tcatgcgtga tgcaatgaaa gtgccgttct
       61 acttcggtgg gacctcactg cttatcgttg ttgtcgtgat tatggacttt atggctcaag
      121 tgcaaactct gatgatgtcc agtcagtatg agtctgcatt gaagaaggcg aacctgaaag
      181 gctacggccg ttaattggtc gcctgagaag ttacggagag taaaaatgaa agttcgtgct
      241 tccgtcaaga aattatgccg taactgcaaa atcgttaagc gtgatggtgt catccgtgtg
      301 atttgcagtg ccgagccgaa gcataaacag cgccaaggct gattttttcg catatttttc
      361 ttgcaaagtt gggttgagct ggctagatta gccagccaat cttttgtatg tctgtacgtt
      421 tccatttgag tatcctgaaa acgggctttt cagcatggta cgtacatatt aaatagtagg
      481 agtgcatagt ggcccgtata gcaggcatta acattcctga tcagaaacac gccgtgatcg
      541 cgttaacttc gatctacggt gtcggcaaga cccgttctaa agccatcctg gctgcagcgg
      601 gtatcgctga aaatgttaag atcctctaga tttaagaagg agatatacat atggtgagca
      661 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
      721 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
      781 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
      841 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
      901 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
      961 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
     1021 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
     1081 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     1141 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     1201 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
     1261 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     1321 gccgccactc caccggcggc atggacgagc tgtacaagta agcatgcaag cttccaggca
     1381 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     1441 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
     1501 taaagcttaa tgcggtagtt tatcacagtt aaattgctaa cgcagtcagg caccgtgtat
     1561 gaaatctaac aatgcgctca tcgtcatcct cggcaccgtc accctggatg ctgtaggcat
     1621 aggcttggtt atgccggtac tgccgggcct cttgcgggat atcgtccatt ccgacagcat
     1681 cgccagtcac tatggcgtgc tgctagcgct tttagccgct ttagcggcct ttccccctac
     1741 ccgaagggtg ggggcgcgtg tgcagccccg cagggcctgt ctcggtcgat cattcagccc
     1801 ggctcatcct tctggcgtgg cggcagaccg aacaaggcgc ggtcgtggtc gcgttcaagg
     1861 tacgcatcca ttgccgccat gagccgatcc tccggccact cgctgctgtt caccttggcc
     1921 aaaatcatgg cccccaccag caccttgcgc cttgtttcgt tcttgcgctc ttgctgctgt
     1981 tcccttgccc gcacccgctg aatttcggca ttgattcgcg ctcgttgttc ttcgagcttg
     2041 gccagccgat ccgccgcctt gttgctcccc ttaaccatct tgacacccca ttgttaatgt
     2101 gctgtctcgt aggctatcat ggaggcacag cggcggcaat cccgacccta ctttgtaggg
     2161 gagggcgcac ttaccggttt ctcttcgaga aactggccta acggccaccc ttcgggcggt
     2221 gcgctctccg agggccattg catggagccg aaaagcaaaa gcaacagcga ggcagcatgg
     2281 cgatttatca ccttacggcg aaaaccggca gcaggtcggg cggccaatcg gccagggcca
     2341 aggccgacta catccagcgc gaaggcaagt atgcccgcga catggatgaa gtcttgcacg
     2401 ccgaatccgg gcacatgccg gagttcgtcg agcggcccgc cgactactgg gatgctgccg
     2461 acctgtatga acgcgccaat gggcggctgt tcaaggaggt cgaatttgcc ctgccggtcg
     2521 agctgaccct cgaccagcag aaggcgctgg cgtccgagtt cgcccagcac ctgaccggtg
     2581 ccgagcgcct gccgtatacg ctggccatcc atgccggtgg cggcgagaac ccgcactgcc
     2641 acctgatgat ctccgagcgg atcaatgacg gcatcgagcg gcccgccgct cagtggttca
     2701 agcggtacaa cggcaagacc ccggagaagg gcggggcaca gaagaccgaa gcgctcaagc
     2761 ccaaggcatg gcttgagcag acccgcgagg catgggccga ccatgccaac cgggcattag
     2821 agcgggctgg ccacgacgcc cgcattgacc acagaacact tgaggcgcag ggcatcgagc
     2881 gcctgcccgg tgttcacctg gggccgaacg tggtggagat ggaaggccgg ggcatccgca
     2941 ccgaccgggc agacgtggcc ctgaacatcg acaccgccaa cgcccagatc atcgacttac
     3001 aggaataccg ggaggcaata gaccatgaac gcaatcgaca gagtgaagaa atccagaggc
     3061 atcaacgagt tagcggagca gatcgaaccg ctggcccaga gcatggcgac actggccgac
     3121 gaagcccggc aggtcatgag ccagacccag caggccagcg aggcgcaggc ggcggagtgg
     3181 ctgaaagccc agcgccagac aggggcggca tgggtggagc tggccaaaga gttgcgggag
     3241 gtagccgccg aggtgagcag cgccgcgcag agcgcccgga gcgcgtcgcg ggggtggcac
     3301 tggaagctat ggctaaccgt gatgctggct tccatgatgc ctacggtggt gctgctgatc
     3361 gcatcgttgc tcttgctcga cctgacgcca ctgacaaccg aggacggctc gatctggctg
     3421 cgcttggtgg cccgatgaag aacgacagga ctttgcaggc cataggccga cagctcaagg
     3481 ccatgggctg tgagcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     3541 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     3601 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     3661 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     3721 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     3781 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     3841 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     3901 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     3961 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     4021 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     4081 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     4141 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     4201 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     4261 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     4321 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     4381 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     4441 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     4501 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     4561 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     4621 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     4681 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     4741 ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     4801 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     4861 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     4921 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     4981 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     5041 cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca
     5101 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     5161 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     5221 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     5281 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     5341 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     5401 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     5461 taacctataa aaataggcgt atcacgaggc cctttcgtct tcaagaa
//
