LOCUS       sequence-211980-       16421 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses dCas9-KRAB tagged with mCherry: 
            pHR-hEF1a-dCas9-mcherry-KRAB-mPGK-Hyg.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16421)
  AUTHORS   Richardson CD, Kazane KR, Feng SJ, Zelin E, Bray NL, Schafer AJ, 
            Floor SN, Corn JE
  TITLE     CRISPR-Cas9 genome editing in human cells occurs via the Fanconi 
            anemia pathway.
  JOURNAL   Nat Genet. 2018 Aug;50(8):1132-1139. doi: 10.1038/s41588-018-0174-0.
            Epub 2018 Jul 27.
  PUBMED    30054595
REFERENCE   2  (bases 1 to 16421)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41588-018-0174-0"; journalName: "Nat Genet"; date: 
            "2018-08"; volume: "50"; issue: "8"; pages: "1132-1139"
COMMENT     Sequence Label: pCR1063
FEATURES             Location/Qualifiers
     source          1..16421
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2050..2167
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2222..3399
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2453..3391
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3348..3368
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3449..3458
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3455..3481,3482..3484,3485..5971,5972..5974,
                     5975..7558)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             3455..7558
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7562..7588
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7568..7594
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             7607..7627
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7634..7654
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7694..8398
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(7836..7854)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     8104..8123
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             8447..8632
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEP"
     promoter        8705..9204
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8740..8763)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     9137..9156
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             9222..10247
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             9222..10247
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     misc_feature    10285..10873
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10338..10358)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10756..10767)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10876..10892)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10877..10893)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10983..11216
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(11241..11259)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(11242..11259)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(11549..11568)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(11782..11801)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11901..11923
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(11961..11979)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        12047..12151
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12152..12220,12221..13012)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12370..12389)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13183..13771
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13672..13691
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13925..13942
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(14012..14032)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        14017..14346
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      14197..14332
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          15480..15545
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             15675..15695
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    16120..16254
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(16157..16176)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     16211..16230
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccaaa tggcagtatt
     2041 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     2101 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     2161 aaattttcgg gtttattaca gggacagcag agatccagtt tggatcgata agcttgatat
     2221 cgaattctgt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
     2281 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
     2341 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
     2401 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
     2461 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
     2521 tacttccacc tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg
     2581 ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg
     2641 gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc
     2701 tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg
     2761 gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc
     2821 cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg
     2881 agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc
     2941 tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc
     3001 agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag
     3061 gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc
     3121 gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga
     3181 ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat
     3241 ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta
     3301 attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac
     3361 agtggttcaa agtttttttc ttccatttca ggtgtcgtgg aattctcacg cgtctgcagg
     3421 atatcaagct tgcggtaccg cgggcccggc caccatggac aagaagtaca gcatcggcct
     3481 ggccatcggc accaactctg tgggctgggc cgtgatcacc gacgagtaca aggtgcccag
     3541 caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga acctgatcgg
     3601 cgccctgctg ttcgacagcg gagaaacagc cgaggccacc cggctgaaga gaaccgccag
     3661 aagaagatac accagacgga agaaccggat ctgctatctg caagagatct tcagcaacga
     3721 gatggccaag gtggacgaca gcttcttcca cagactggaa gagtccttcc tggtggaaga
     3781 ggataagaag cacgagcggc accccatctt cggcaacatc gtggacgagg tggcctacca
     3841 cgagaagtac cccaccatct accacctgag aaagaaactg gtggacagca ccgacaaggc
     3901 cgacctgcgg ctgatctatc tggccctggc ccacatgatc aagttccggg gccacttcct
     3961 gatcgagggc gacctgaacc ccgacaacag cgacgtggac aagctgttca tccagctggt
     4021 gcagacctac aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg tggacgccaa
     4081 ggccatcctg tctgccagac tgagcaagag cagacggctg gaaaatctga tcgcccagct
     4141 gcccggcgag aagaagaatg gcctgttcgg caacctgatt gccctgagcc tgggcctgac
     4201 ccccaacttc aagagcaact tcgacctggc cgaggatgcc aaactgcagc tgagcaagga
     4261 cacctacgac gacgacctgg acaacctgct ggcccagatc ggcgaccagt acgccgacct
     4321 gtttctggcc gccaagaacc tgtccgacgc catcctgctg agcgacatcc tgagagtgaa
     4381 caccgagatc accaaggccc ccctgagcgc ctctatgatc aagagatacg acgagcacca
     4441 ccaggacctg accctgctga aagctctcgt gcggcagcag ctgcctgaga agtacaaaga
     4501 gattttcttc gaccagagca agaacggcta cgccggctac atcgatggcg gagccagcca
     4561 ggaagagttc tacaagttca tcaagcccat cctggaaaag atggacggca ccgaggaact
     4621 gctcgtgaag ctgaacagag aggacctgct gcggaagcag cggaccttcg acaacggcag
     4681 catcccccac cagatccacc tgggagagct gcacgccatt ctgcggcggc aggaagattt
     4741 ttacccattc ctgaaggaca accgggaaaa gatcgagaag atcctgacct tccgcatccc
     4801 ctactacgtg ggccctctgg ccaggggaaa cagcagattc gcctggatga ccagaaagag
     4861 cgaggaaacc atcaccccct ggaacttcga ggaagtggtg gacaagggcg ccagcgccca
     4921 gagcttcatc gagcggatga ccaacttcga taagaacctg cccaacgaga aggtgctgcc
     4981 caagcacagc ctgctgtacg agtacttcac cgtgtacaac gagctgacca aagtgaaata
     5041 cgtgaccgag ggaatgagaa agcccgcctt cctgagcggc gagcagaaaa aagccatcgt
     5101 ggacctgctg ttcaagacca accggaaagt gaccgtgaag cagctgaaag aggactactt
     5161 caagaaaatc gagtgcttcg actccgtgga aatctccggc gtggaagatc ggttcaacgc
     5221 ctccctgggc acataccacg atctgctgaa aattatcaag gacaaggact tcctggacaa
     5281 tgaggaaaac gaggacattc tggaagatat cgtgctgacc ctgacactgt ttgaggacag
     5341 agagatgatc gaggaacggc tgaaaaccta tgcccacctg ttcgacgaca aagtgatgaa
     5401 gcagctgaag cggcggagat acaccggctg gggcaggctg agccggaagc tgatcaacgg
     5461 catccgggac aagcagtccg gcaagacaat cctggatttc ctgaagtccg acggcttcgc
     5521 caacagaaac ttcatgcagc tgatccacga cgacagcctg acctttaaag aggacatcca
     5581 gaaagcccag gtgtccggcc agggcgatag cctgcacgag cacattgcca atctggccgg
     5641 cagccccgcc attaagaagg gcatcctgca gacagtgaag gtggtggacg agctcgtgaa
     5701 agtgatgggc cggcacaagc ccgagaacat cgtgatcgaa atggccagag agaaccagac
     5761 cacccagaag ggacagaaga acagccgcga gagaatgaag cggatcgaag agggcatcaa
     5821 agagctgggc agccagatcc tgaaagaaca ccccgtggaa aacacccagc tgcagaacga
     5881 gaagctgtac ctgtactacc tgcagaatgg gcgggatatg tacgtggacc aggaactgga
     5941 catcaaccgg ctgtccgact acgatgtgga cgctatcgtg cctcagagct ttctgaagga
     6001 cgactccatc gataacaaag tgctgactcg gagcgacaag aaccggggca agagcgacaa
     6061 cgtgccctcc gaagaggtcg tgaagaagat gaagaactac tggcgccagc tgctgaatgc
     6121 caagctgatt acccagagga agttcgacaa tctgaccaag gccgagagag gcggcctgag
     6181 cgaactggat aaggccggct tcatcaagag acagctggtg gaaacccggc agatcacaaa
     6241 gcacgtggca cagatcctgg actcccggat gaacactaag tacgacgaga acgacaaact
     6301 gatccgggaa gtgaaagtga tcaccctgaa gtccaagctg gtgtccgatt tccggaagga
     6361 tttccagttt tacaaagtgc gcgagatcaa caactaccac cacgcccacg acgcctacct
     6421 gaacgccgtc gtgggaaccg ccctgatcaa aaagtaccct aagctggaaa gcgagttcgt
     6481 gtacggcgac tacaaggtgt acgacgtgcg gaagatgatc gccaagagcg agcaggaaat
     6541 cggcaaggct accgccaagt acttcttcta cagcaacatc atgaactttt tcaagaccga
     6601 gattaccctg gccaacggcg agatccggaa gcggcctctg atcgagacaa acggcgaaac
     6661 aggcgagatc gtgtgggata agggccggga ctttgccacc gtgcggaaag tgctgtctat
     6721 gccccaagtg aatatcgtga aaaagaccga ggtgcagaca ggcggcttca gcaaagagtc
     6781 tatcctgccc aagaggaaca gcgacaagct gatcgccaga aagaaggact gggaccctaa
     6841 gaagtacggc ggcttcgaca gccccaccgt ggcctattct gtgctggtgg tggccaaagt
     6901 ggaaaagggc aagtccaaga aactgaagag tgtgaaagag ctgctgggga tcaccatcat
     6961 ggaaagaagc agcttcgaga agaatcccat cgactttctg gaagccaagg gctacaaaga
     7021 agtgaaaaag gacctgatca tcaagctgcc taagtactcc ctgttcgagc tggaaaacgg
     7081 ccggaagaga atgctggcct ctgccggcga actgcagaag ggaaacgaac tggccctgcc
     7141 ctccaaatat gtgaacttcc tgtacctggc cagccactat gagaagctga agggctcccc
     7201 cgaggataat gagcagaaac agctgtttgt ggaacagcac aaacactacc tggacgagat
     7261 catcgagcag atcagcgagt tctccaagag agtgatcctg gccgacgcta atctggacaa
     7321 ggtgctgagc gcctacaaca agcacagaga caagcctatc agagagcagg ccgagaatat
     7381 catccacctg tttaccctga ccaatctggg agcccctgcc gccttcaagt actttgacac
     7441 caccatcgac cggaagaggt acaccagcac caaagaggtg ctggacgcca ccctgatcca
     7501 ccagagcatc accggcctgt acgagacacg gatcgacctg tctcagctgg gaggcgacgc
     7561 ctatccctat gacgtgcccg attatgccag cctgggcagc ggctccccca agaaaaaacg
     7621 caaggtggaa gatcctaaga aaaagcggaa agtggacggc attggtagtg ggagcaacgg
     7681 cagcagcgga tccgtgagca agggcgagga ggataacatg gccatcatca aggagttcat
     7741 gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
     7801 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg
     7861 ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta
     7921 cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa
     7981 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc
     8041 cctccaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga
     8101 cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc
     8161 cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca
     8221 ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc
     8281 ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga
     8341 acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagaa
     8401 tggcggtggc ggagggatgg atgctaagtc actaactgcc tggtcccgga cactggtgac
     8461 cttcaaggat gtatttgtgg acttcaccag ggaggagtgg aagctgctgg acactgctca
     8521 gcagatcgtg tacagaaatg tgatgctgga gaactataag aacctggttt ccttgggtta
     8581 tcagcttact aagccagatg tgatcctccg gttggagaag ggagaagagc cctagtaagg
     8641 ccgcgactct agaaattctg cagatatccg gactagtgat ctctcgaggt taacgaattc
     8701 taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg
     8761 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt
     8821 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta
     8881 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta
     8941 gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc
     9001 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga
     9061 aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg
     9121 tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc
     9181 tcttcctcat ctccgggcct ttcgacctgc atcccgccac catgaaaaag cctgaactca
     9241 ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc
     9301 agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg
     9361 tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact
     9421 ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc
     9481 tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg
     9541 aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc
     9601 ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat
     9661 ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg
     9721 acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg
     9781 actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg
     9841 acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat
     9901 acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc
     9961 gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc
    10021 tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag
    10081 cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta
    10141 cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg
    10201 atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaatagcct gcaggcatgc
    10261 aagcttgata tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg
    10321 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
    10381 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
    10441 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
    10501 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
    10561 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
    10621 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
    10681 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
    10741 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
    10801 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
    10861 gccgcctccc cgcatcgata ccgtcgacct cgagggaatt aattcgagct cggtaccttt
    10921 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
    10981 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
    11041 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
    11101 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
    11161 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagcat
    11221 ctagaattaa ttccgtgtat tctatagtgt cacctaaatc gtatgtgtat gatacataag
    11281 gttatgtatt aattgtagcc gcgttctaac gacaatatgt acaagcctaa ttgtgtagca
    11341 tctggcttac tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg
    11401 gttggacgaa ccttctgagt ttctggtaac gccgtcccgc acccggaaat ggtcagcgaa
    11461 ccaatcagca gggtcatcgc tagccagatc ctctacgccg gacgcatcgt ggccggcatc
    11521 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
    11581 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc
    11641 gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg
    11701 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag
    11761 cgtcgaatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
    11821 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
    11881 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
    11941 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
    12001 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
    12061 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
    12121 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
    12181 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
    12241 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
    12301 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
    12361 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
    12421 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
    12481 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
    12541 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
    12601 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
    12661 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
    12721 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
    12781 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
    12841 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
    12901 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
    12961 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
    13021 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
    13081 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
    13141 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
    13201 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
    13261 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
    13321 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
    13381 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
    13441 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
    13501 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
    13561 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
    13621 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
    13681 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
    13741 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
    13801 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
    13861 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
    13921 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
    13981 cgcgcgttgg ccgattcatt aatgcagctg tggaatgtgt gtcagttagg gtgtggaaag
    14041 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
    14101 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
    14161 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
    14221 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
    14281 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
    14341 tgcaaaaagc ttggacacaa gacaggcttg cgagatatgt ttgagaatac cactttatcc
    14401 cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac tggtttttag
    14461 tgcgccagat ctctataatc tcgcgcaacc tattttcccc tcgaacactt tttaagccgt
    14521 agataaacag gctgggacac ttcacatgag cgaaaaatac atcgtcacct gggacatgtt
    14581 gcagatccat gcacgtaaac tcgcaagccg actgatgcct tctgaacaat ggaaaggcat
    14641 tattgccgta agccgtggcg gtctgtaccg ggtgcgttac tggcgcgtga actgggtatt
    14701 cgtcatgtcg ataccgtttg tatttccagc tacgatcacg acaaccagcg cgagcttaaa
    14761 gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga cctggtggat
    14821 accggtggta ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc
    14881 ttcgcaaaac cggctggtcg tccgctggtt gatgactatg ttgttgatat cccgcaagat
    14941 acctggattg aacagccgtg ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc
    15001 taatcttttc aacgcctggc actgccgggc gttgttcttt ttaacttcag gcgggttaca
    15061 atagtttcca gtaagtattc tggaggctgc atccatgaca caggcaaacc tgagcgaaac
    15121 cctgttcaaa ccccgcttta aacatcctga aacctcgacg ctagtccgcc gctttaatca
    15181 cggcgcacaa ccgcctgtgc agtcggccct tgatggtaaa accatccctc actggtatcg
    15241 catgattaac cgtctgatgt ggatctggcg cggcattgac ccacgcgaaa tcctcgacgt
    15301 ccaggcacgt attgtgatga gcgatgccga acgtaccgac gatgatttat acgatacggt
    15361 gattggctac cgtggcggca actggattta tgagtgggcc ccggatcttt gtgaaggaac
    15421 cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg
    15481 taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat
    15541 tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg
    15601 aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc
    15661 aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag
    15721 aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta
    15781 tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatattctg
    15841 taacctttat aagtaggcat aacagttata atcataacat actgtttttt cttactccac
    15901 acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc tttagctttt
    15961 taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact agagatcata
    16021 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
    16081 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
    16141 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
    16201 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg
    16261 ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc
    16321 aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa
    16381 agaaattgta tttgttaaat atgtactaca aacttagtag t
//
