LOCUS       sequence-211989-        9991 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of EYFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9991)
  AUTHORS   Stuurman N, Pacios Bras C, Schlaman HR, Wijfjes AH, Bloemberg G, 
            Spaink HP
  TITLE     Use of green fluorescent protein color variants expressed on stable 
            broad-host-range vectors to visualize rhizobia interacting with 
            plants.
  JOURNAL   Mol Plant Microbe Interact. 2000 Nov;13(11):1163-9. doi: 
            10.1094/MPMI.2000.13.11.1163.
  PUBMED    11059482
REFERENCE   2  (bases 1 to 9991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI.2000.13.11"; journalName: "Mol Plant Microbe 
            Interact"; date: "2000-11"; volume: "13"; issue: "11"; pages: 
            "1163-9"
COMMENT     Sequence Label: pMP4639
FEATURES             Location/Qualifiers
     source          1..9991
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(88..93,94..110,111..116))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    386..407
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(422..427,428..445,446..452)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    460..476
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     465..487
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     484..500
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     484..500
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     519..539
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        521..539
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     568..584
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     569..585
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      637..646
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(643..645,646..648,649..1362)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(688..709)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(949..968)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1296..1317
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2095..2112)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2229..2774)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(2309..2328)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      3483..3677
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(3743..4816)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(5245..5874)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             complement(7080..8279)
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             8385..9035
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     primer_bind     complement(9528..9547)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 atgaaaaccg ccactgcgcc gttaccaccg ctgcgttcgg tcaaggttct ggaccagttg
       61 cgtgagcgca tacgctactt gcattacagt ttacgaaccg aacaggctta tgtcaactgg
      121 gttcgtgcct tcatccgttt ccacggtgtg cgtccatggg caaatattat acgcaaggcg
      181 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg
      241 tcggcagaat gcttaatgaa ttacaacagt ttttatgcat gcgcccaata cgcaaaccgc
      301 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
      361 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
      421 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
      481 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
      541 aacaaaagct gggtaccggg ccccccctcg aggtcgacgg tatcgataag cttgatatcg
      601 aattcctgca gcccggggga tccccgggta ccggtcgcca ccatggtgag caagggcgag
      661 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
      721 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
      781 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcggc
      841 tacggcgtgc agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag
      901 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
      961 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     1021 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     1081 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     1141 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     1201 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc
     1261 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     1321 gccgccggga tcactctcgg catggacgag ctgtacaagt aaagcggccg cgactctaga
     1381 gcggccgcca ccgcggtgga gctcgaattc gaagcttttg ccattctcac cggattcagt
     1441 cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg
     1501 ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg
     1561 gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat
     1621 tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc
     1681 agaattggtt aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgg
     1741 ctttgttgaa taaatcgaac ttttgctgag ttgaaggatc agatcacgca tcttcccgac
     1801 aacgcagacc gttccgtggc aaagcaaaag ttcaaaatca ccaactggtc cacctacaac
     1861 aaagctctca tcaaccgtgg ctccctcact ttctggctgg atgatggggc gattcaggcc
     1921 tggtatgagt cagcaacacc ttcttcacga ggcagacctc agcgctagcg gagtgtatac
     1981 tggcttacta tgttggcact gatgagggtg tcagtgaagt gcttcatgtg gcaggagaaa
     2041 aaaggctgca ccggtgcgtc agcagaatat gtgatacagg atatattccg cttcctcgct
     2101 cactgactcg ctacgctcgg tcgttcgact gcggcgagcg gaaatggctt acgaacgggg
     2161 cggagatttc ctggaagatg ccaggaagat acttaacagg gaagtgagag ggccgcggca
     2221 aagccgtttt tccataggct ccgcccccct gacaagcatc acgaaatctg acgctcaaat
     2281 cagtggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggcggctcc
     2341 ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg tcattccgct gttatggccg
     2401 cgtttgtctc attccacgcc tgacactcag ttccgggtag gcagttcgct ccaagctgga
     2461 ctgtatgcac gaaccccccg ttcagtccga ccgctgcgcc ttatccggta actatcgtct
     2521 tgagtccaac ccggaaagac atgcaaaagc accactggca gcagccactg gtaattgatt
     2581 tagaggagtt agtcttgaag tcatgcgccg gttaaggcta aactgaaagg acaagttttg
     2641 gtgactgcgc tcctccaagc cagttacctc ggttcaaaga gttggtagct cagagaacct
     2701 tcgaaaaacc gccctgcaag gcggtttttt cgttttcaga gcaagagatt acgcgcagac
     2761 caaaacgatc tcaagaagat catcttatta aggggtctga cgctcagtgg aacgaaaact
     2821 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     2881 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     2941 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     3001 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     3061 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     3121 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     3181 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     3241 ttgttgccat tgcctggtat gagtcagcaa caccttcttc acgaggcaga cctcagcgcc
     3301 ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc
     3361 cagccatttt tgagcggcca gcggccgcga taggccgacg cgaagcggcg gggcgtaggg
     3421 agcgcagcga ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca
     3481 gttatgcaca ggccaggcgg gttttaagag ttttaataag ttttaaagag ttttaggcgg
     3541 aaaaatcgcc ttttttctct tttatatcag tcacttacat gtgtgaccgg ttcccaatgt
     3601 acggctttgg gttcccaatg tacgggttcc ggttcccaat gtacggcttt gggttcccaa
     3661 tgtacgtgct atccacagga aagagacctt ttcgaccttt ttcccctgct agggcaattt
     3721 gccctagcat ctgctccgta cattaggaac cggcggatgc ttcgccctcg atcaggttgc
     3781 ggtagcgcat gactaggatc gggccagcct gccccgcctc ctccttcaaa tcgtactccg
     3841 gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca gaacttcttg aactctccgg
     3901 cgctgccact gcgttcgtag atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg
     3961 cgcggcgtgc caggcggtag agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg
     4021 ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac atccaatcag
     4081 ctagctcgat ctcgatgtac tccggccgcc cggtttcgct ctttacgatc ttgtagcggc
     4141 taatcaaggc ttcaccctcg gataccgtca ccaggcggcc gttcttggcc ttcttcgtac
     4201 gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct
     4261 gctttccgcc atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca
     4321 cgcggccggg cttgtctccc ttcccttccc ggtatcggtt catggattcg gttagatggg
     4381 aaaccgccat cagtaccagg tcgtaatccc acacactggc catgccggcc ggccctgcgg
     4441 aaacctctac gtgcccgtct ggaagctcgt agcggatcac ctcgccagct cgtcggtcac
     4501 gcttcgacag acggaaaacg gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt
     4561 catagagcat cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg
     4621 acggcgcacc ggctgccggc ggttgccggg attctttgcg gattcgatca gcggccgctt
     4681 gccacgattc accggggcgt gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg
     4741 ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc gggccggatg
     4801 gtttgcgacc gctcacgccg attcctcggg cttgggggtt ccagtgccat tgcagggccg
     4861 gcagacaacc cagccgctta cgcctggcca accgcccgtt cctccacaca tggggcattc
     4921 cacggcgtcg gtgcctggtt gttcttgatt ttccatgccg cctcctttag ccgctaaaat
     4981 tcatctactc atttattcat ttgctcattt actctggtag ctgcgcgatg tattcagata
     5041 gcagctcggt aatggtcttg ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct
     5101 ccgccggcaa ctgaaagttg acccgcttca tggctggcgt gtctgccagg ctggccaacg
     5161 ttgcagcctt gctgctgcgt gcgctcggac ggccggcact tagcgtgttt gtgcttttgc
     5221 tcattttctc tttacctcat taactcaaat gagttttgat ttaatttcag cggccagcgc
     5281 ctggacctcg cgggcagcgt cgccctcggg ttctgattca agaacggttg tgccggcggc
     5341 ggcagtgcct gggtagctca cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta
     5401 cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac
     5461 gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa ccagctccac
     5521 caggtcggcg gtggcccata tgtcgtaagg gcttggctgc accggaatca gcacgaagtc
     5581 ggctgccttg atcgcggaca cagccaagtc cgccgcctgg ggcgctccgt cgatcactac
     5641 gaagtcgcgc cggccgatgg ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac
     5701 aacggttagc ggttgatctt cccgcacggc cgcccaatcg cgggcactgc cctggggatc
     5761 ggaatcgact aacagaacat cggccccggc gagttgcagg gcgcgggcta gatgggttgc
     5821 gatggtcgtc ttgcctgacc cgcctttctg gttaagtaca gcgataacct tcatgcgttc
     5881 cccttgcgta tttgtttatt tactcatcgc atcatatacg cagcgaccgc atgacgcaag
     5941 ctgttttact caaatacaca tcaccttttt agacggcggc gctcggtttc ttcagcggcc
     6001 aagctggccg gccaggccgc cagcttggca tcagacaaac cggccaggat ttcatgcagc
     6061 cgcacggttg agacgtgcgc gggcggctcg aacacgtacc cggccgcgat catctccgcc
     6121 tcgatctctt cggtaatgaa aaacggttcg tcctggccgt cctggtgcgg tttcatgctt
     6181 gttcctcttg gcgttcattc tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct
     6241 cacggaaggc accgcgccgc ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg
     6301 cgcggtacag ggtcgagcga tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc
     6361 cttcctggtc gatcagctcg cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg
     6421 ggccaaactt cacgcctcgg gccttggcgg cctcgcgccc gctccgggtg cggtcgatga
     6481 ttagggaacg ctcgaactcg gcaatgccgg cgaacacggt caacaccatg cggccggccg
     6541 gcgtggtggt gtcggcccac ggctctgcca ggctacgcag gcccgcgccg gcctcctgga
     6601 tgcgctcggc aatgtccagt aggtcgcggg tgctgcgggc caggcggtct agcctggtca
     6661 ctgtcacaac gtcgccaggg cgtaggtggt caagcatcct ggccagctcc gggcggtcgc
     6721 gcctggtgcc ggtgatcttc tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg
     6781 cccgttggtt ggtcaagtcc tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag
     6841 cggcggcgct cttgttcatg gcgtaatgtc tccggttcta gtcgcaagta ttctacttta
     6901 tgcgactaaa acacgcgaca agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg
     6961 taacttagga cttgtgcgac atgtcgtttt cagaagacgg ctgcactgaa cgtcagaagc
     7021 cgactgcact atagcagcgg aggggttgga tccatatgac gtccctatcg tttccacgat
     7081 cagcgatcgg ctcgttgccc tgcgccgctc caaagcccgc gacgcagcgc cggcaggcag
     7141 agcaagtaga gggcagcgcc tgcaatccat gcccacccgt tccacgttgt tatagaagcc
     7201 gcatagatcg ccgtgaagag gaggggtccg acgatcgagg tcaggctggt gagcgccgcc
     7261 agtgagcctt gcagctgccc ctgacgttcc tcatccacct gcctggacaa cattgcttgc
     7321 agcgccggca ttccgatgcc acccgaagca agcaggacca tgatcgggaa cgccatccat
     7381 ccccgtgtcg cgaaggcaag caggatgtag cctgtgccgt cggcaatcat tccgagcatg
     7441 agtgcccgcc tttcgccgag ccgggcggct acagggccgg tgatcattgc ctgggcgagt
     7501 gaatgcagaa tgccaaatgc ggcaagcgaa atgccgatcg tggtcgcgtc ccagtgaaag
     7561 cgatcctcgc cgaaaatgac ccaaagcgcg gccggcacct gtccgacaag ttgcatgatg
     7621 aagaagaccg ccatcagggc ggcgacgacg gtcatgcccc gggcccaccg gaacgaagcg
     7681 agcgggttga gagcctcccg gcgtaacggc cggcgttcgc ctttgtgcga ctccggcaaa
     7741 aggaaacagc ccgtcaggaa attgaggccg ttcaaggctg ccgcggcgaa gaacggagcg
     7801 tggggggaga aaccgcccat cagcccaccg agcacaggtc ccgcgaccat cccgaacccg
     7861 aaacaggcgc tcatgaagcc gaagtgccgc gcgcgctcat cgccatcagt gatatcggca
     7921 atataagcgc cggctaccgc cccagtcgcc ccggtgatgc cggccacgat ccgcccgata
     7981 tagagaaccc aaaggaaagg cgctgtcgcc atgatggcgt agtcgacagt ggcgccggcc
     8041 agcgagacga gcaagattgg ccgccgcccg aaacgatccg acagcgcgcc cagcacaggt
     8101 gcgcaggcaa attgcaccaa cgcatacagc gccagcagaa tgccatagtg ggcggtgacg
     8161 tcgttcgagt gaaccagatc gcgcaggagg cccggcagca ccggcataat caggccgatg
     8221 ccgacagcgt cgagcgcgac agtgctcaga attacgatca ggggtatgtt gggtttcacg
     8281 tctggcctcc ggaccagcct ccgctggtcc gattgaacgc gcggattctt tatcactgat
     8341 aagttggtgg acatattatg tttatcagtg ataaagtgtc aagcatgaca aagttgcagc
     8401 cgaatacagt gatccgtgcc gccctggacc tgttgaacga ggtcggcgta gacggtctga
     8461 cgacacgcaa actggcggaa cggttggggg ttcagcagcc ggcgctttac tggcacttca
     8521 ggaacaagcg ggcgctgctc gacgcactgg ccgaagccat gctggcggag aatcatacgc
     8581 attcggtgcc gagagccgac gacgactggc gctcatttct gatcgggaat gcccgcagct
     8641 tcaggcaggc gctgctcgcc taccgcgatg gcgcgcgcat ccatgccggc acgcgaccgg
     8701 gcgcaccgca gatggaaacg gccgacgcgc agcttcgctt cctctgcgag gcgggttttt
     8761 cggccgggga cgccgtcaat gcgctgatga caatcagcta cttcactgtt ggggccgtgc
     8821 ttgaggagca ggccggcgac agcgatgccg gcgagcgcgg cggcaccgtt gaacaggctc
     8881 cgctctcgcc gctgttgcgg gccgcgatag acgccttcga cgaagccggt ccggacgcag
     8941 cgttcgagca gggactcgcg gtgattgtcg atggattggc gaaaaggagg ctcgttgtca
     9001 ggaacgttga aggaccgaga aagggtgacg attgatcagg accgctgccg gagcgcaacc
     9061 cactcactac agcagagcca tgtagacaac atcccctccc cctttccacc gcgtcagacg
     9121 cccgtagcag cccgctacgg gctttttcat gccctgccct agcgtccaag cctcacggcc
     9181 gcgctcggcc tctctggcgg ccttctggcg ctgaggtctg cctcgtgaag aaggtgttgc
     9241 tgactcatac caggcctgaa tcgccccatc atccagccag aaagtgaggg agccacggtt
     9301 gatgagagct ttgttgtagg tggaccagtt ggtgattttg aacttttgct ttgccacgga
     9361 acggtctgcg ttgtcgggaa gatgcgtgat ctgatccttc aactcagcaa aagttcgatt
     9421 tattcaacaa agccacgttg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa
     9481 tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat
     9541 gagccatatt caacgggaaa cgtcttgctc gagatctcac tacgcggctg ctcaaacttg
     9601 ggcagaacgt aagccgcgag agcgccaaca accgcttctt ggtcgaaggc agcaagcgcg
     9661 atgaatgtct tactacggag caagttcccg aggtaatcgg agtccggctg atgttgggag
     9721 taggtggcta cgtctccgaa ctcacgaccg aaaagatcaa gagcagcccg catggatttg
     9781 acttggtcag ggccgagcct acatgtgcga atgatgccca tacttgagcc acctaacttt
     9841 gttttagggc gactgccctg ctgcgtaaca tcgttgctgc tgcgtaacat cgttgctgct
     9901 ccataacatc aaacatcgac ccacggcgta acgcgcttgc tgcttggatg cccgaggcat
     9961 agactgtaca aaaaaacagt cataacaagc c
//
