LOCUS       sequence-21199-e         746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Taube R, Lin X, Irwin D, Fujinaga K, Peterlin BM
  TITLE     Interaction between P-TEFb and the C-terminal domain of RNA 
            polymerase II activates transcriptional elongation from sites 
            upstream or downstream of target genes.
  JOURNAL   Mol Cell Biol. 2002 Jan . 22(1):321-31.
  PUBMED    11739744
REFERENCE   2  (bases 1 to 746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2002 Jan . 22(1):321-31."
COMMENT     Sequence Label: pCMV Flag cyclin T1 (P#1716)
FEATURES             Location/Qualifiers
     source          1..746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..74
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        55..73
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             96..119
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 attagcgagc tctctggcta ctagagaacc cactgcttac tggcttatcg aaattaatac
       61 gactcactat agggagaccc aagcttggta ccatggatta caaggacgac gatgacaagg
      121 aattccccat ggagggagag aggaagaaca acaacaaacg gtggtatttc actcgagaac
      181 agctggaaaa tagcccatcc cgtcgttttg gcgtggaccc agataaagaa ctttcttatc
      241 gccagcaggc ggccaatctg cttcaggaca tggggcagcg tcttaacgtc tcacaattga
      301 ctatcaacac tgctatagta tacatgcatc gattctacat gattcagtcc ttcacacagt
      361 tccctggaaa ttctgtggct ccagcagcct tgtttctagc agctaaagtg gaggagcagc
      421 ccaaaaaatt ggaacatgtc atcaaggtag cacatacttg tctccatcct caggaatccc
      481 ttcctgatac tagaagtgag gcttatttgc aacaagttca agatctggtc attttagaaa
      541 gcataatttt gcagacttta ggctttgaac taacaattga tcacccacat actcatgtag
      601 taaagtgcac tcaacttgtt cgagcaagca aggacttagc acagacttct tacttcatgg
      661 caaccaacag cctgcatttg accacattta gcctgcagta cacacctcct gtggtggcct
      721 gtgtctgcat ccactggctg gcagtg
//
