LOCUS       sequence-21200-e         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Taube R, Lin X, Irwin D, Fujinaga K, Peterlin BM
  TITLE     Interaction between P-TEFb and the C-terminal domain of RNA 
            polymerase II activates transcriptional elongation from sites 
            upstream or downstream of target genes.
  JOURNAL   Mol Cell Biol. 2002 Jan . 22(1):321-31.
  PUBMED    11739744
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2002 Jan . 22(1):321-31."
COMMENT     Sequence Label: pEF myc Gal CDK9 (P#1475)
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             87..104
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             114..143
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             159..599
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             615..644
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 tctttcggtg tcgtgaggat tagcttgctt acatttgctt ctgacacaac tgtgttcact
       61 agcaacctca aacagacacc atgccgcatc accaccatca tcacgccatg ccggagcaga
      121 agctgatatc cgaggaggac ctggccatgg ccggatccat gaagctactg tcttctatcg
      181 aacaagcatg cgatatttgc cgacttaaaa agctcaagtg ctccaaagaa aaaccgaagt
      241 gcgccaagtg tctgaagaac aactgggagt gtcgctactc tcccaaaacc aaaaggtctc
      301 cgctgactag ggcacatctg acagaagtgg aatcaaggct agaaagactg gaacagctat
      361 ttctactgat ttttcctcga gaagaccttg acatgatttt gaaaatggat tctttacagg
      421 atataaaagc attgttaaca ggattatttg tacaagataa tgtgaataaa gatgccgtca
      481 cagatagatt ggcttcagtg gagactgata tgcctctaac attgagacag catagaataa
      541 gtgcgacatc atcatcggaa gagagtagta acaaaggtca aagacagttg actgtatcgc
      601 cggaattcat gccggagcag aagctgatat ccgaggagga cctggccatg gcaaagcagt
      661 acgactcggt ggagtgccct ttttgtgaga agtttccaaa tacgagaagc tcgccaagat
      721 cggccaggca ccttcgggga gtgtncangc caggcacgca agaccggcca gaagtgctct
      781 gagaacgcgc tgaggaaacg agaagagggt cccatacgct ttngaancag ncttc
//
