LOCUS       sequence-212001-       10673 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  human VEGFR2 with V5 and tGFP tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10673)
  AUTHORS   Raikwar NS, Shibuya M, Thomas CP
  TITLE     VEGF-A selectively inhibits FLT1 ectodomain shedding independent of 
            receptor activation and receptor endocytosis.
  JOURNAL   Am J Physiol Cell Physiol. 2018 Aug 1;315(2):C214-C224. doi: 
            10.1152/ajpcell.00247.2017. Epub 2018 May 2.
  PUBMED    29719170
REFERENCE   2  (bases 1 to 10673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1152/ajpcell.00247.2017"; journalName: "Am J Physiol Cell 
            Physiol"; date: "2018-08-1- 1"; volume: "315"; issue: "2"; pages: 
            "C214-C224"
COMMENT     Sequence Label: hKDR
FEATURES             Location/Qualifiers
     source          1..10673
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     151..173
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     165..182
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     166..182
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        343..722
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        723..926
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     877..897
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     952..971
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        952..970
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1092..1133
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5160..5855
                     /codon_start=1
                     /product="improved green fluorescent protein from 
                     Pontellina plumata (Evdokimov et al., 2006)"
                     /label=TurboGFP
                     /note="mammalian codon-optimized"
                     /translation="MESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKM
                     KSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVL
                     HVSFSYRYEAGRVIGDFKVMGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTR
                     TFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFAFRRVEEDHSNTELGIVEYQHAF
                     KTPDADAGEE"
     primer_bind     complement(5878..5894)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    5905..6527
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(6104..6123)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(6551..6571)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6556..6885
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6736..6871
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6952..7746
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7006..7025)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7616..7635
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7920..8041
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7957..7976)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8011..8030
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8090..8106)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8103..8125)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8114..8130
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8138..8144,8145..8162,8163..8168))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8183..8204
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8321..8338)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8492..9080)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8572..8591)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9251..10042,10043..10111))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9874..9893
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10112..10216)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      10243..10671
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10330..10349)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10540..10561
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gcagagcaag gtgctgctgg ccgtcgccct gtggctctgc gtggagaccc
     1081 gggccgcctc tggtaagcct atccctaacc ctctcctcgg tctcgattct acggtgggtt
     1141 tgcctagtgt ttctcttgat ctgcccaggc tcagcataca aaaagacata cttacaatta
     1201 aggctaatac aactcttcaa attacttgca ggggacagag ggacttggac tggctttggc
     1261 ccaataatca gagtggcagt gagcaaaggg tggaggtgac tgagtgcagc gatggcctct
     1321 tctgtaagac actcacaatt ccaaaagtga tcggaaatga cactggagcc tacaagtgct
     1381 tctaccggga aactgacttg gcctcggtca tttatgtcta tgttcaagat tacagatctc
     1441 catttattgc ttctgttagt gaccaacatg gagtcgtgta cattactgag aacaaaaaca
     1501 aaactgtggt gattccatgt ctcgggtcca tttcaaatct caacgtgtca ctttgtgcaa
     1561 gatacccaga aaagagattt gttcctgatg gtaacagaat ttcctgggac agcaagaagg
     1621 gctttactat tcccagctac atgatcagct atgctggcat ggtcttctgt gaagcaaaaa
     1681 ttaatgatga aagttaccag tctattatgt acatagttgt cgttgtaggg tataggattt
     1741 atgatgtggt tctgagtccg tctcatggaa ttgaactatc tgttggagaa aagcttgtct
     1801 taaattgtac agcaagaact gaactaaatg tggggattga cttcaactgg gaataccctt
     1861 cttcgaagca tcagcataag aaacttgtaa accgagacct aaaaacccag tctgggagtg
     1921 agatgaagaa atttttgagc accttaacta tagatggtat aacccggagt gaccaaggat
     1981 tgtacacctg tgcagcatcc agtgggctga tgaccaagaa gaacagcaca tttgtcaggg
     2041 tccatgaaaa accttttgtt gcttttggaa gtggcatgga atctctggtg gaagccacgg
     2101 tgggggagcg tgtcagaatc cctgcgaagt accttggtta cccaccccca gaaataaaat
     2161 ggtataaaaa tggaataccc cttgagtcca atcacacaat taaagcgggg catgtactga
     2221 cgattatgga agtgagtgaa agagacacag gaaattacac tgtcatcctt accaatccca
     2281 tttcaaagga gaagcagagc catgtggtct ctctggttgt gtatgtccca ccccagattg
     2341 gtgagaaatc tctaatctct cctgtggatt cctaccagta cggcaccact caaacgctga
     2401 catgtacggt ctatgccatt cctcccccgc atcacatcca ctggtattgg cagttggagg
     2461 aagagtgcgc caacgagccc agccaagctg tctcagtgac aaacccatac ccttgtgaag
     2521 aatggagaag tgtggaggac ttccagggag gaaataaaat tgaagttaat aaaaatcaat
     2581 ttgctctaat tgaaggaaaa aacaaaactg taagtaccct tgttatccaa gcggcaaatg
     2641 tgtcagcttt gtacaaatgt gaagcggtca acaaagtcgg gagaggagag agggtgatct
     2701 ccttccacgt gaccaggggt cctgaaatta ctttgcaacc tgacatgcag cccactgagc
     2761 aggagagcgt gtctttgtgg tgcactgcag acagatctac gtttgagaac ctcacatggt
     2821 acaagcttgg cccacagcct ctgccaatcc atgtgggaga gttgcccaca cctgtttgca
     2881 agaacttgga tactctttgg aaattgaatg ccaccatgtt ctctaatagc acaaatgaca
     2941 ttttgatcat ggagcttaag aatgcatcct tgcaggacca aggagactat gtctgccttg
     3001 ctcaagacag gaagaccaag aaaagacatt gcgtggtcag gcagctcaca gtcctagagc
     3061 gtgtggcacc cacgatcaca ggaaacctgg agaatcagac gacaagtatt ggggaaagca
     3121 tcgaagtctc atgcacggca tctgggaatc cccctccaca gatcatgtgg tttaaagata
     3181 atgagaccct tgtagaagac tcaggcattg tattgaagga tgggaaccgg aacctcacta
     3241 tccgcagagt gaggaaggag gacgaaggcc tctacacctg ccaggcatgc agtgttcttg
     3301 gctgtgcaaa agtggaggca tttttcataa tagaaggtgc ccaggaaaag acgaacttgg
     3361 aaatcattat tctagtaggc acggcggtga ttgccatgtt cttctggcta cttcttgtca
     3421 tcatcctacg gaccgttaag cgggccaatg gaggggaact gaagacaggc tacttgtcca
     3481 tcgtcatgga tccagatgaa ctcccattgg atgaacattg tgaacgactg ccttatgatg
     3541 ccagcaaatg ggaattcccc agagaccggc tgaagctagg taagcctctt ggccgtggtg
     3601 cctttggcca agtgattgaa gcagatgcct ttggaattga caagacagca acttgcagga
     3661 cagtagcagt caaaatgttg aaagaaggag caacacacag tgagcatcga gctctcatgt
     3721 ctgaactcaa gatcctcatt catattggtc accatctcaa tgtggtcaac cttctaggtg
     3781 cctgtaccaa gccaggaggg ccactcatgg tgattgtgga attctgcaaa tttggaaacc
     3841 tgtccactta cctgaggagc aagagaaatg aatttgtccc ctacaagacc aaaggggcac
     3901 gattccgtca agggaaagac tacgttggag caatccctgt ggatctgaaa cggcgcttgg
     3961 acagcatcac cagtagccag agctcagcca gctctggatt tgtggaggag aagtccctca
     4021 gtgatgtaga agaagaggaa gctcctgaag atctgtataa ggacttcctg accttggagc
     4081 atctcatctg ttacagcttc caagtggcta agggcatgga gttcttggca tcgcgaaagt
     4141 gtatccacag ggacctggcg gcacgaaata tcctcttatc ggagaagaac gtggttaaaa
     4201 tctgtgactt tggcttggcc cgggatattt ataaagatcc agattatgtc agaaaaggag
     4261 atgctcgcct ccctttgaaa tggatggccc cagaaacaat ttttgacaga gtgtacacaa
     4321 tccagagtga cgtctggtct tttggtgttt tgctgtggga aatattttcc ttaggtgctt
     4381 ctccatatcc tggggtaaag attgatgaag aattttgtag gcgattgaaa gaaggaacta
     4441 gaatgagggc ccctgattat actacaccag aaatgtacca gaccatgctg gactgctggc
     4501 acggggagcc cagtcagaga cccacgtttt cagagttggt ggaacatttg ggaaatctct
     4561 tgcaagctaa tgctcagcag gatggcaaag actacattgt tcttccgata tcagagactt
     4621 tgagcatgga agaggattct ggactctctc tgcctacctc acctgtttcc tgtatggagg
     4681 aggaggaagt atgtgacccc aaattccatt atgacaacac agcaggaatc agtcagtatc
     4741 tgcagaacag taagcgaaag agccggcctg tgagtgtaaa aacatttgaa gatatcccgt
     4801 tagaagaacc agaagtaaaa gtaatcccag atgacaacca gacggacagt ggtatggttc
     4861 ttgcctcaga agagctgaaa actttggaag acagaaccaa attatctcca tcttttggtg
     4921 gaatggtgcc cagcaaaagc agggagtctg tggcatctga aggctcaaac cagacaagcg
     4981 gctaccagtc cggatatcac tccgatgaca cagacaccac cgtgtactcc agtgaggaag
     5041 cagaactttt aaagctgata gagattggag tgcaaaccgg tagcacagcc cagattctcc
     5101 agcctgactc ggggaccaca ctgagctctc ctcctgttac gcgtacgcgg ccgctcgaga
     5161 tggagagcga cgagagcggc ctgcccgcca tggagatcga gtgccgcatc accggcaccc
     5221 tgaacggcgt ggagttcgag ctggtgggcg gcggagaggg cacccccgag cagggccgca
     5281 tgaccaacaa gatgaagagc accaaaggcg ccctgacctt cagcccctac ctgctgagcc
     5341 acgtgatggg ctacggcttc taccacttcg gcacctaccc cagcggctac gagaacccct
     5401 tcctgcacgc catcaacaac ggcggctaca ccaacacccg catcgagaag tacgaggacg
     5461 gcggcgtgct gcacgtgagc ttcagctacc gctacgaggc cggccgcgtg atcggcgact
     5521 tcaaggtgat gggcaccggc ttccccgagg acagcgtgat cttcaccgac aagatcatcc
     5581 gcagcaacgc caccgtggag cacctgcacc ccatgggcga taacgatctg gatggcagct
     5641 tcacccgcac cttcagcctg cgcgacggcg gctactacag ctccgtggtg gacagccaca
     5701 tgcacttcaa gagcgccatc caccccagca tcctgcagaa cgggggcccc atgttcgcct
     5761 tccgccgcgt ggaggaggat cacagcaaca ccgagctggg catcgtggag taccagcacg
     5821 ccttcaagac cccggatgca gatgccggtg aagaaagagt ttaaacggcc ggccgcggtc
     5881 atagctgttt cctgaacaga tcccgggtgg catccctgtg acccctcccc agtgcctctc
     5941 ctggccctgg aagttgccac tccagtgccc accagccttg tcctaataaa attaagttgc
     6001 atcattttgt ctgactaggt gtccttctat aatattatgg ggtggagggg ggtggtatgg
     6061 agcaaggggc aagttgggaa gacaacctgt agggcctgcg gggtctattg ggaaccaagc
     6121 tggagtgcag tggcacaatc ttggctcact gcaatctccg cctcctgggt tcaagcgatt
     6181 ctcctgcctc agcctcccga gttgttggga ttccaggcat gcatgaccag gctcagctaa
     6241 tttttgtttt tttggtagag acggggtttc accatattgg ccaggctggt ctccaactcc
     6301 taatctcagg tgatctaccc accttggcct cccaaattgc tgggattaca ggcgtgaacc
     6361 actgctccct tccctgtcct tctgatttta aaataactat accagcagga ggacgtccag
     6421 acacagcata ggctacctgg ccatgcccaa ccggtgggac atttgagttg cttgcttggc
     6481 actgtcctct catgcgttgg gtccactcag tagatgcctg ttgaattggg tacgcggcca
     6541 gcttggctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     6601 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     6661 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     6721 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     6781 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     6841 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
     6901 tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa
     6961 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     7021 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     7081 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
     7141 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     7201 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     7261 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     7321 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     7381 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     7441 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
     7501 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     7561 tctggattca tcgactgtgg ccggctgggt gtggccgacc gctatcagga catagcgttg
     7621 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     7681 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     7741 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     7801 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     7861 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
     7921 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     7981 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     8041 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     8101 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     8161 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     8221 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     8281 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     8341 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     8401 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     8461 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     8521 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     8581 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     8641 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     8701 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     8761 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     8821 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     8881 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     8941 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     9001 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     9061 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     9121 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     9181 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     9241 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     9301 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     9361 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     9421 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     9481 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     9541 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     9601 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     9661 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     9721 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     9781 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     9841 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     9901 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     9961 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
    10021 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
    10081 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    10141 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    10201 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg
    10261 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
    10321 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
    10381 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
    10441 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
    10501 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
    10561 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
    10621 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat ttt
//
