LOCUS       sequence-212079-       12736 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12736)
  AUTHORS   Zhang F, Cong L, Lodato S, Kosuri S, Church GM, Arlotta P
  TITLE     Efficient construction of sequence-specific TAL effectors for 
            modulating mammalian transcription.
  JOURNAL   Nat Biotechnol. 2011 Feb;29(2):149-53. doi: 10.1038/nbt.1775
  PUBMED    21248753
REFERENCE   2  (bases 1 to 12736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2011-02"; volume: "29"; issue:
            "2"; pages: "149-53"
COMMENT     Sequence Label: pLenti-EF1a-TALE1
FEATURES             Location/Qualifiers
     source          1..12736
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1606..1723
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1848..3026
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2079..3017
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2974..2994
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             5583..5603
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5628..5777
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             5799..5852
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5853..5855,5856..6569)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(5853..5855,5856..6566)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(5895..5916)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6156..6175)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6503..6524
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6594..7182
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6647..6667)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7065..7076)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7185..7201)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7186..7202)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7707..7887
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7913..7930)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7919..8143
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8189..8617
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8276..8295)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8486..8507
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8626..8646)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8631..8960
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8811..8946
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8873..8892
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9008..9055
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9074..9448
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9578..9699
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9615..9634)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9669..9688
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9748..9764)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9748..9764)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9761..9783)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9772..9788
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9796..9802,9803..9820,9821..9826))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9841..9862
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9979..9996)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10150..10738)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10230..10249)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10909..11700,11701..11769))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11532..11551
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11770..11874)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11949..11968)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        12140..12519
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12521..12719
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     12674..12694
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca
     1561 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg
     1621 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     1681 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     1741 cagcagagat ccagtttggt taattaatgc aaagatggat aaagttttaa acagagagga
     1801 atctttgcag ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc
     1861 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
     1921 attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
     1981 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
     2041 acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc
     2101 gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc
     2161 tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc
     2221 cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg
     2281 gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc
     2341 tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg
     2401 taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg
     2461 gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga
     2521 gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc
     2581 cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg
     2641 aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg
     2701 gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg
     2761 cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct
     2821 tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca
     2881 ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat
     2941 ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt
     3001 tttttcttcc atttcaggtg tcgtgacgta cggccaccat gtcgcggacc cggctccctt
     3061 ccccacccgc acccagccca gcgttttcgg ccgactcgtt ctcagacctg cttaggcagt
     3121 tcgacccctc actgtttaac acatcgttgt tcgactccct tcctccgttt ggggcgcacc
     3181 atacggaggc ggccaccggg gagtgggatg aggtgcagtc gggattgaga gctgcggatg
     3241 caccaccccc aaccatgcgg gtggccgtca ccgctgcccg accgccgagg gcgaagcccg
     3301 caccaaggcg gagggcagcg caaccgtccg acgcaagccc cgcagcgcaa gtagatttga
     3361 gaactttggg atattcacag cagcagcagg aaaagatcaa gcccaaagtg aggtcgacag
     3421 tcgcgcagca tcacgaagcg ctggtgggtc atgggtttac acatgcccac atcgtagcct
     3481 tgtcgcagca ccctgcagcc cttggcacgg tcgccgtcaa gtaccaggac atgattgcgg
     3541 cgttgccgga agccacacat gaggcgatcg tcggtgtggg gaaacagtgg agcggagccc
     3601 gagcgcttga ggccctgttg acggtcgcgg gagagctgag agggcctccc cttcagctgg
     3661 acacgggcca gttgctgaag atcgcgaagc ggggaggagt cacggcggtc gaggcggtgc
     3721 acgcgtggcg caatgcgctc acgggagcac ccctcaacct gaccccagag caggtcgtgg
     3781 caatcgcctc caacattggc gggaaacagg cactcgagac tgtccagcgc ctgcttcccg
     3841 tgctgtgcca agcgcacgga ctcaccccag agcaggtcgt ggcgatcgca agccacgacg
     3901 gaggaaagca agccttggaa acagtacaga ggctgttgcc tgtgctgtgc caagcgcacg
     3961 gcctcacccc agagcaggtc gtggcaatcg cgagcaataa cggcggaaaa caggctttgg
     4021 aaacggtgca gaggctcctt ccagtgctgt gccaagcgca cggattaacc ccagagcagg
     4081 tcgtggcaat cgcctccaac attggcggga aacaggcact cgagactgtc cagcgcctgc
     4141 ttcccgtgct gtgccaagcg cacggcttaa ccccagagca ggtcgtggcg atcgcaagcc
     4201 acgacggagg aaagcaagcc ttggaaacag tacagaggct gttgcctgtg ctgtgccaag
     4261 cgcacggact taccccagag caggtcgtgg ccattgcctc gaatggaggg ggcaaacagg
     4321 cgttggaaac cgtacaacga ttgctgccgg tgctgtgcca agcgcacggc cttaccccag
     4381 agcaggtcgt ggcgatcgca agccacgacg gaggaaagca agccttggaa acagtacaga
     4441 ggctgttgcc tgtgctgtgc caagcgcacg gactaacccc agagcaggtc gtggcaatcg
     4501 cctccaacat tggcgggaaa caggcactcg agactgtcca gcgcctgctt cccgtgctgt
     4561 gccaagcgca cgggctcacc ccagagcagg tcgtggcgat cgcaagccac gacggaggaa
     4621 agcaagcctt ggaaacagta cagaggctgt tgcctgtgct gtgccaagcg cacgggctaa
     4681 ccccagagca ggtcgtggcc attgcctcga atggaggggg caaacaggcg ttggaaaccg
     4741 tacaacgatt gctgccggtg ctgtgccaag cgcacggcct aaccccagag caggtcgtgg
     4801 caatcgcctc caacattggc gggaaacagg cactcgagac tgtccagcgc ctgcttcccg
     4861 tgctgtgcca agcgcacggg ttaaccccag agcaggtcgt ggccattgcc tcgaatggag
     4921 ggggcaaaca ggcgttggaa accgtacaac gattgctgcc ggtgctgtgc caagcgcacg
     4981 gactcacgcc tgagcaggta gtggctattg catccaatat cgggggcaga cccgcactgg
     5041 agtcaatcgt ggcccagctt tcgaggccgg accccgcgct ggccgcactc actaatgatc
     5101 atcttgtagc gctggcctgc ctcggcggac gacccgcctt ggatgcggtg aagaaggggc
     5161 tcccgcacgc gcctgcattg attaagcgga ccaacagaag gattcccgag aggacatcac
     5221 atcgagtggc agatcacgcg caagtggtcc gcgtgctcgg attcttccag tgtcactccc
     5281 accccgcaca agcgttcgat gacgccatga ctcaatttgg tatgtcgaga cacggactgc
     5341 tgcagctctt tcgtagagtc ggtgtcacag aactcgaggc ccgctcgggc acactgcctc
     5401 ccgcctccca gcggtgggac aggattctcc aagcgagcgg tatgaaacgc gcgaagcctt
     5461 cacctacgtc aactcagaca cctgaccagg cgagccttca tgcgttcgca gactcgctgg
     5521 agagggattt ggacgcgccc tcgcccatgc atgaagggga ccaaactcgc gcgtcagcta
     5581 gccccaagaa gaagagaaag gtggaggcca gcggttccgg acgggctgac gcattggacg
     5641 attttgatct ggatatgctg ggaagtgacg ccctcgatga ttttgacctt gacatgcttg
     5701 gttcggatgc ccttgatgac tttgacctcg acatgctcgg cagtgacgcc cttgatgatt
     5761 tcgacctgga catgctgatt aactctagag gcagtggaga gggcagagga agtctgctaa
     5821 catgcggtga cgtcgaggag aatcctggcc cagtgagcaa gggcgaggag ctgttcaccg
     5881 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     5941 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     6001 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
     6061 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     6121 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     6181 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     6241 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     6301 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     6361 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     6421 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
     6481 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     6541 ctctcggcat ggacgagctg tacaagtaag aattcgatat caagcttatc gataatcaac
     6601 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     6661 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     6721 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     6781 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     6841 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     6901 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     6961 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     7021 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     7081 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     7141 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc
     7201 gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt
     7261 gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac
     7321 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     7381 ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct
     7441 accacacaca aggctacttc cctgattggc agaactacac accagggcca gggatcagat
     7501 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagcaagag aaggtagaag
     7561 aagccaatga aggagagaac acccgcttgt tacaccctgt gagcctgcat gggatggatg
     7621 acccggagag agaagtatta gagtggaggt ttgacagccg cctagcattt catcacatgg
     7681 cccgagagct gcatccggac tgtactgggt ctctctggtt agaccagatc tgagcctggg
     7741 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     7801 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     7861 tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc agcctcgact
     7921 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     7981 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     8041 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     8101 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     8161 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
     8221 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     8281 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     8341 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     8401 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     8461 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     8521 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     8581 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
     8641 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     8701 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     8761 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     8821 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     8881 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
     8941 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
     9001 agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt
     9061 gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt
     9121 cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga
     9181 cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca
     9241 ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc
     9301 cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga
     9361 gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt
     9421 gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc
     9481 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     9541 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     9601 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     9661 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     9721 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     9781 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     9841 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     9901 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     9961 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
    10021 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
    10081 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
    10141 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
    10201 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
    10261 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
    10321 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
    10381 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
    10441 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
    10501 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    10561 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
    10621 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    10681 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    10741 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    10801 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
    10861 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
    10921 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
    10981 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
    11041 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
    11101 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    11161 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    11221 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    11281 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    11341 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    11401 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    11461 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    11521 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    11581 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    11641 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    11701 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    11761 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    11821 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    11881 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc
    11941 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt
    12001 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg
    12061 accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta
    12121 cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg
    12181 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
    12241 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
    12301 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
    12361 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
    12421 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
    12481 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
    12541 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
    12601 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
    12661 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcagc
    12721 gcgttttgcc tgtact
//
