LOCUS       sequence-212098-        9185 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of synthetic human USP7 tagged with GST.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9185)
  AUTHORS   Faesen AC, Dirac AM, Shanmugham A, Ovaa H, Perrakis A, Sixma TK
  TITLE     Mechanism of USP7/HAUSP activation by its C-terminal ubiquitin-like 
            domain and allosteric regulation by GMP-synthetase.
  JOURNAL   Mol Cell. 2011 Oct 7;44(1):147-59. doi: 
            10.1016/j.molcel.2011.06.034.
  PUBMED    21981925
REFERENCE   2  (bases 1 to 9185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2011.06"; journalName: "Mol Cell"; date: 
            "2011-10-7- 7"; volume: "44"; issue: "1"; pages: "147-59"
COMMENT     Sequence Label: pFastbac1-GST-hUSP7
FEATURES             Location/Qualifiers
     source          1..9185
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      2..456
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(89..108)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     298..319
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        482..586
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(587..655,656..1447)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(805..824)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1618..2206
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2107..2126
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2360..2377
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2511..2735
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2802..3335)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3524..3529,3530..3546,3547..3552))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    4060..4108
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4133..4152
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     promoter        complement(4244..4353)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        4377..4468
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     4393..4410
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             4511..5164
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     5122..5144
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             5171..5194
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     polyA_signal    8675..8809
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8712..8731)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8766..8785
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  8838..9003
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
       61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
      241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt cttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
      481 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
      541 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
      601 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
      661 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
      721 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
      781 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
      841 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
      901 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
      961 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     1021 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     1081 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     1141 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     1201 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     1261 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     1321 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     1381 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     1441 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     1501 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     1561 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     1621 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     1681 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     1741 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     1801 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     1861 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     1921 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     1981 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     2041 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     2101 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     2161 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     2221 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     2281 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     2341 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg
     2401 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct
     2461 ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga
     2521 caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag
     2581 acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt
     2641 tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga
     2701 ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac
     2761 aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg
     2821 tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg
     2881 ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca
     2941 tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact
     3001 gcgagatcat agatatagat ctcactacgc ggctgctcaa acttgggcag aacgtaagcc
     3061 gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta
     3121 cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct
     3181 ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg
     3241 agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg
     3301 ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca
     3361 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa
     3421 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     3481 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca
     3541 ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
     3601 cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc
     3661 ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg
     3721 cacggatctg cccttgcttc aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt
     3781 ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt
     3841 gttcgcccag gactctagct atagttctag tggttggcta cgtacccgta gtggctatgg
     3901 cagggcttgc cgccccgacg ttggctgcga gccctgggcc ttcacccgaa cttgggggtt
     3961 ggggtgggga aaaggaagaa acgcgggcgt attggtccca atggggtctc ggtggggtat
     4021 cgacagagtg ccagccctgg gaccgaaccc cgcgtttatg aacaaacgac ccaacacccg
     4081 tgcgttttat tctgtctttt tattgccgtc atagcgcggg ttccttccgg tattgtctcc
     4141 ttccgtgttt cagttagcct cccccatctc ccggtaccgc atgctatgca tcagctgcta
     4201 gcaccatggc tcgagatccc gggtgatcaa gtcttcgtcg agtgattgta aataaaatgt
     4261 aatttacagt atagtatttt aattaatata caaatgattt gataataatt cttatttaac
     4321 tataatatat tgtgttgggt tgaattaaag gtccgtatac tccggaatat taatagatca
     4381 tggagataat taaaatgata accatctcgc aaataaataa gtattttact gttttcgtaa
     4441 cagttttgta ataaaaaaac ctataaatat tccggattat tcataccgtc ccaccatcgg
     4501 gcgcggatcc atgtccccta tactaggtta ttggaaaatt aagggccttg tgcaacccac
     4561 tcgacttctt ttggaatatc ttgaagaaaa atatgaagag catttgtatg agcgcgatga
     4621 aggtgataaa tggcgaaaca aaaagtttga attgggtttg gagtttccca atcttcctta
     4681 ttatattgat ggtgatgtta aattaacaca gtctatggcc atcatacgtt atatagctga
     4741 caagcacaac atgttgggtg gttgtccaaa agagcgtgca gagatttcaa tgcttgaagg
     4801 agcggttttg gatattagat acggtgtttc gagaattgca tatagtaaag actttgaaac
     4861 tctcaaagtt gattttctta gcaagctacc tgaaatgctg aaaatgttcg aagatcgttt
     4921 atgtcataaa acatatttaa atggtgatca tgtaacccat cctgacttca tgttgtatga
     4981 cgctcttgat gttgttttat acatggaccc aatgtgcctg gatgcgttcc caaaattagt
     5041 ttgttttaaa aaacgtattg aagctatccc acaaattgat aagtacttga aatccagcaa
     5101 gtatatagca tggcctttgc agggctggca agccacgttt ggtggtggcg accatcctcc
     5161 aaaatcggat ctggaagttc tgttccaggg gccaactagt atgaaccacc agcaacagca
     5221 gcagcaacag aaagcaggtg aacagcaact ctctgaaccg gaagatatgg aaatggaagc
     5281 aggtgatacc gacgatccac cgcgtattac ccagaacccg gttattaacg gtaacgttgc
     5341 attaagcgat ggtcacaaca ccgcagaaga agacatggaa gatgacacta gctggcgtag
     5401 cgaagcaacc ttccagttca ccgttgaacg tttctctcgt ctgagcgaaa gcgttctgtc
     5461 tccaccgtgc ttcgttcgta acttaccgtg gaaaatcatg gttatgccgc gtttctatcc
     5521 ggatcgtccg catcagaaaa gcgttggctt cttcctgcag tgcaacgcag aaagcgatag
     5581 cacctcctgg agctgccacg cacaggcagt tctgaaaatc attaactacc gtgatgatga
     5641 aaagagcttc tctcgtcgta ttagccactt attctttcac aaagagaacg attggggttt
     5701 cagcaacttc atggcatggt ctgaagtgac tgatcctgaa aagggtttca tcgacgatga
     5761 caaagtgact ttcgaagtgt tcgttcaggc agatgcacca catggtgtag cttgggacag
     5821 caagaaacac actggctatg taggtttaaa gaaccagggt gcaacctgct acatgaactc
     5881 tctgctccag accctgttct ttaccaacca gcttcgtaaa gcagtttaca tgatgcctac
     5941 ggaaggtgat gacagctcca aatctgtacc acttgctctg cagcgtgtgt tctacgagtt
     6001 acagcactct gataaaccag ttggtaccaa aaaactgacc aaatccttcg gttgggaaac
     6061 cctggacagc ttcatgcagc atgatgttca agaactgtgt cgtgtactcc tggataacgt
     6121 tgagaacaag atgaaaggca cgtgcgtgga aggcaccatt ccgaaattat tccgtggcaa
     6181 aatggtaagc tacattcagt gcaaagaagt tgactaccgt agcgatcgtc gcgaagacta
     6241 ctatgacatt cagctctcca tcaaaggtaa gaaaaacatc ttcgaatcct tcgttgacta
     6301 cgttgcagtt gaacagttag atggtgacaa caaatacgat gctggcgaac atggtctgca
     6361 ggaagcagaa aaaggtgtga agttcctgac tctgcctccg gttcttcatc tgcaactgat
     6421 gcgcttcatg tatgatccgc agaccgatca gaacatcaaa atcaacgatc gtttcgaatt
     6481 tcctgaacag ttaccactgg atgagttctt gcagaaaact gatccgaaag atcctgcgaa
     6541 ctacattctg catgctgttc tggttcacag cggtgacaat catggcggtc attatgttgt
     6601 gtacttaaac cctaaaggtg atggcaaatg gtgcaaattc gatgacgatg ttgtgtctcg
     6661 ctgcaccaaa gaagaagcta ttgaacacaa ctatggtggt cacgacgatg acctgtctgt
     6721 acgtcactgc accaacgcat atatgttagt gtacattcgt gaaagcaaac tgagcgaagt
     6781 tctgcaggca gttaccgatc acgacattcc acagcagctc gttgaacgtc tgcaggaaga
     6841 gaaacgcatt gaagctcaga aacgcaaaga acgtcaggaa gcacacttat acatgcaggt
     6901 tcagatcgtg gctgaagatc agttctgtgg tcatcagggc aacgatatgt acgatgaaga
     6961 gaaagttaaa tacaccgtgt tcaaagttct gaaaaacccc tctcttgcag aatttgtaca
     7021 gtccttaagc cagacgatgg gcttccctca ggaccagatt cgtctgtggc cgatgcaggc
     7081 acgtagcaac ggtaccaaac gtccagcgat gctggacaac gaagcagatg gcaacaaaac
     7141 catgattgaa ctcagcgaca atgaaaaccc ttggacgatc ttcttagaaa ctgttgatcc
     7201 ggaactggca gcgtctggtg cgactcttcc gaaactcgac aaagaccacg acgttatgct
     7261 gttcctgaaa atgtacgatc cgaaaactcg ctcgttgaac tactgtggtc acatctacac
     7321 tccgatctcc tgcaaaattc gtgacctgtt accagtgatg tgtgatcgtg ctggcttcat
     7381 tcaggacacc tctctgattc tctacgaaga agtgaaaccg aacctgaccg aacgcatcca
     7441 ggactacgat gtgtctctgg acaaagcgtt agatgagttg atggatggtg acatcattgt
     7501 gttccagaaa gacgatcctg aaaacgacaa ctctgaactt ccgactgcta aagaatactt
     7561 tcgtgatctg tatcaccgtg tagatgtgat cttctgcgac aaaaccattc cgaacgatcc
     7621 aggctttgtg gtaaccttat ccaatcgcat gaactacttt caggttgcga aaactgttgc
     7681 tcagcgtctg aacactgatc cgatgctgct tcagttcttc aaatctcaag gctatcgtga
     7741 tggtcctggc aatcctttac gtcacaacta tgaaggcact ctgcgtgatt tgctgcagtt
     7801 cttcaaacct cgtcagccaa agaaactgta ctatcagcaa ctgaagatga agatcaccga
     7861 cttcgaaaac cgtcgcagct tcaaatgcat ctggttaaac tctcagtttc gcgaagaaga
     7921 gatcactctg tacccagaca aacatggctg tgttcgtgat ctgctcgaag aatgcaagaa
     7981 agctgtagaa cttggcgaaa aagcgtctgg caaacttcgt ctgctcgaaa ttgtgagcta
     8041 caaaatcatc ggtgtgcatc aagaagatga actgttagaa tgtctgtctc cagcgaccag
     8101 ccgtaccttt cgtattgaag aaattcctct tgatcaggtt gacattgaca aagaaaacga
     8161 aatgctggtt accgttgcac acttccacaa agaagtgttt ggtaccttcg gtattccgtt
     8221 tctgttacgc attcatcaag gtgaacactt tcgtgaagcg atgaaacgca ttcagtctct
     8281 gctggacatc caggagaaag agttcgaaaa gttcaaattt gctattgtta tgactggtcg
     8341 tcatcagtac atcaacgaag atgaatatga agtgaactta aaagacttcg aacctcagcc
     8401 aggcaacatg agccatccac gtccgtggct tggtctggac cacttcaaca aagctccgaa
     8461 acgctctcgt tacacctact tagaaaaagc gatcaaaatt cacaactaag cggccgcttt
     8521 cgaatctaga gcctgcagtc tcgacaagct tgtcgagaag tactagagga tcataatcag
     8581 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     8641 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     8701 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     8761 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatct gatcactgct
     8821 tgagcctagg agatccgaac cagataagtg aaatctagtt ccaaactatt ttgtcatttt
     8881 taattttcgt attagcttac gacgctacac ccagttccca tctattttgt cactcttccc
     8941 taaataatcc ttaaaaactc catttccacc cctcccagtt cccaactatt ttgtccgccc
     9001 acagcggggc atttttcttc ctgttatgtt tttaatcaaa catcctgcca actccatgtg
     9061 acaaaccgtc atcttcggct actttttctc tgtcacagaa tgaaaatttt tctgtcatct
     9121 cttcgttatt aatgtttgta attgactgaa tatcaacgct tatttgcagc ctgaatggcg
     9181 aatgg
//
