LOCUS       sequence-212110-        4976 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pZE2-hrtR-RBS3-chuA - LuxCDABE transcriptional unit deletion of 
            plasmid pMM643, ColE1 origin, KanR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4976)
  AUTHORS   Mimee M, Nadeau P, Hayward A, Carim S, Flanagan S, Jerger L, Collins
            J, McDonnell S, Swartwout R, Citorik RJ, Bulovic V, Langer R, 
            Traverso G, Chandrakasan AP, Lu TK
  TITLE     An ingestible bacterial-electronic system to monitor 
            gastrointestinal health.
  JOURNAL   Science. 2018 May 25;360(6391):915-918. doi: 
            10.1126/science.aas9315.
  PUBMED    29798884
REFERENCE   2  (bases 1 to 4976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2018-05-25- 25"; volume: "360"; 
            issue: "6391"; pages: "915-918"
COMMENT     Sequence Label: pMM1162
FEATURES             Location/Qualifiers
     source          1..4976
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      785..871
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        complement(2964..2998)
                     /label=BBa_J23107
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.36"
     rep_origin      complement(3159..3747)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3239..3258)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3835..3929)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3960..4754)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4071..4090)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4681..4700
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4933..4951
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cacagctaac accacgtcgt ccctatctgc tgccctaggt ctatgagtgg ttgctggata
       61 actttacggg catgcataag gctcgtataa tatattcagg gagaccacaa cggtttccct
      121 ctacaaataa ttttgtttaa ctttgaattc tatactctaa ttaatcacat aataaggacg
      181 aatttatgcc aaaatcaacc tattttagtc tttctgacga aaaacgaaaa cgtgtctatg
      241 atgcctgttt actagaattt caaacgcact ctttccatga agctaaaatc atgcacatcg
      301 taaaagcact tgatatccca agaggaagtt tttatcaata ctttgaagat ttgaaggatt
      361 catactatta tatcttgtca caggaaactg tcgagattca tgatttattt tttaatttac
      421 taaaagaata tcctctagaa gttgctctta ataaatacaa gtatcttctt cttgaaaatt
      481 tagtaaattc gccccaatat aatctttata aatatcgatt tttagattgg acttatgaat
      541 tagaaagaga ttggaagcct aaaggcgagg taactgttcc cgctcgtgaa cttgataatc
      601 ctatttccca agtattaaaa tcagtcattc acaatctagt ttatcgcatg tttagtgaaa
      661 attgggatga acaaaagttt attgaaactt acgataaaga aatcaaattg ctcacagagg
      721 gcttgcttaa ttatgttact gaaagcaaaa aatagggatc ccatggtacg cgtgctagag
      781 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
      841 gtcggtgaac gctctcctga gtaggacaaa tccgccgccc tagaggacca aaacgaaaaa
      901 aggcccccct ttcgggaggc ctcttttctg gaatttggta ccgagttacc attgataact
      961 cacgaaaatt tttccgttac gaccatcctg tgggatgcct tgcggcgacc agtactcttt
     1021 gtcgaaagcg ttacccaaca ccaaagtagt ggtcatacct ttgagcgcct gttgtccttg
     1081 ataactgacg tagaaatcat tcacgccata gcctggttgt ttgctgtaac tgctgctgat
     1141 atgtgttgag cgatcggcaa acgtaccaac ccacccaaca gagaagccac tgtgagcgat
     1201 cggaatattc agagtgctgg taacagtatc cgggttaatg ctggagatgt attcgccggt
     1261 atcggtgtct ttgccgcggg tacggttata ggccacatca aggctaaaca gatcagtggt
     1321 atatttcgtc atcacatccc agccccagat tttggcgttc gggacgttat acgacatagt
     1381 cgtcgccgcc gcgaaatcga cggtcgtgga gatgtaatcc ttcgctttgg tatcaaagta
     1441 gctggcttta aattccagag catcattgga caacatcagg tcatcaaaac gcagcccaaa
     1501 accgtactcc tgagtttcgt tagtttccgg acgtaagttc gggtttggca cccaatagtt
     1561 ggtatagaag cgaccaatcg agaagtgctt agaatcgtta tacatttcgc ccatcgtcgg
     1621 ggcgcggaat gcctgggcat atgagccaaa taacatcagc cagttagtcg gattgatagt
     1681 catccccgca cgagatgacc atttgtcggc atcaacatct ttgtaaccgt cactgctacc
     1741 gcgataactg tcatagcggg ttccgccaag cagggtaatc ggcagatcgc gtaaggtgat
     1801 ctcatcctgt agccagccgg agctaaaatc gatttttgct tgcgggaagc ccgtcgtcgc
     1861 gccgcccgga tgttgttcct gacgataata ctcaccgcca tatgtcagta agtgagaagc
     1921 gaaactgtcg gcaaagagag tggaacggtt ctccagcctg gctccttttg ttatctgttc
     1981 acgatactcg ccggaactcc ccgtgttttg cgcattaata cggacttccg accaataaat
     2041 ttttgcatct gcatttaacc agtcgttgcc ctgcggggcg agtttataag aaagctgcgc
     2101 atcgcgttga attgttgaac gatcaaccat cgggttgctg ctttcagaag ccccaacggt
     2161 ctgcggattt tttggttcac gcgcgtcgtt gttgtagtaa cgcactaaac cgctcagaga
     2221 ctgggctgaa tcaatttgcc aggtcccttt cgccagcatg ttattaatgg actcgtcatt
     2281 cggcgcggtt tcaccattgc tctggcgtaa atcaccccga tcgcgactgg accaggccac
     2341 aataccatcc agattttcag ttcgcccaaa cgcgctcgcg cctaatccca ggctatggtc
     2401 ccccgtgccg ccagtaccaa agacacgaaa accactgctt tgtccttcct gcaataaatc
     2461 ttttgcatcg accgtatcgt aggagatcac tccacccagc gcgccactgc catacagtaa
     2521 tgctgaaggt ccacgaacaa tctcaacacg cttgatcagc gccggatcga gaaaagtgcc
     2581 attcaggtgt ccggtatccg ttccctgacg aacaccatcg acaagaacca gcacgccgcg
     2641 atgatcatag ccacgcatat ttacatcctg accgttggtt cgtccggtac catccagagt
     2701 aattccagga acatgacgca gcagatcggt ggctgaagta gccgtttgat tttcaggagc
     2761 ggaagtgtcg atgacgctga ccatcatagg cgcttcgaag gaactacggg cattccccgt
     2821 tgccgtaacg gtcatggttt cagtagcaaa agcaaacgtt ggcaaggtgg cagaaacagc
     2881 taaggccaat aaactcaaac gcaacgaggt aaattgcgga cgtgacatga tttaaagctc
     2941 ccttgggtta gtgttggaaa tgtgctagca taatacctag ggctgagcta gccgtaaacc
     3001 taggtagacc taggcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     3061 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     3121 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     3181 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     3241 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     3301 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca
     3361 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     3421 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     3481 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     3541 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     3601 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     3661 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     3721 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     3781 gctcagtgga acgaaaactc acgttaaggg attttggtca tgactagtgc ttggattctc
     3841 accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga
     3901 ggtcattact ggatctatca acaggagtcc aagcgagctc tcgaacccca gagtcccgct
     3961 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
     4021 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
     4081 gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat
     4141 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
     4201 acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg
     4261 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
     4321 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
     4381 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga
     4441 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca
     4501 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc
     4561 gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc
     4621 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt
     4681 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca
     4741 tcttgttcaa tcatgcgaaa cgatcctcat cctgtctctt gatcagatct tgatcccctg
     4801 cgccatcaga tccttggcgg caagaaagcc atccagttta ctttgcaggg cttcccaacc
     4861 ttaccagagg gcgccccagc tggcaattcc gacgtctaag aaaccattat tatcatgaca
     4921 ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcacctcga gatgct
//
