LOCUS       sequence-212121-        9133 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses dLbCpf1-eBE-YE in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9133)
  AUTHORS   Li X, Wang Y, Liu Y, Yang B, Wang X, Wei J, Lu Z, Zhang Y, Wu J, 
            Huang X, Yang L, Chen J
  TITLE     Base editing with a Cpf1-cytidine deaminase fusion.
  JOURNAL   Nat Biotechnol. 2018 Mar 19. pii: nbt.4102. doi: 10.1038/nbt.4102.
  PUBMED    29553573
REFERENCE   2  (bases 1 to 9133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Mar 19. pii: nbt.4102. doi: 10.1038/nbt.4102."
COMMENT     Sequence Label: pCMV-dCpf1-eBE-YE
FEATURES             Location/Qualifiers
     source          1..9133
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        140..343
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     294..314
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     385..404
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        385..403
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             420..440
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4866..4886
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4899..5147
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5160..5180
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5187..5240
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5241..5489
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5490..5510
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5511..5567
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5568..5816
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5817..5837
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5838..5891
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5892..6140
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6153..6173
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6196..6213)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6202..6426
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6497..6513)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6497..6513)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6510..6532)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6521..6537
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6545..6551,6552..6569,6570..6575))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6590..6611
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6728..6745)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6899..7487)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6979..6998)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7658..8449,8450..8518))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8281..8300
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8519..8623)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8702..8721)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(8893..9133,1..139)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
       61 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
      121 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
      181 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      241 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
      301 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca
      361 gatccgctag agatccgcgg ccgctaatac gactcactat agggagagcc gccaccatgc
      421 ccaagaagaa gaggaaagtc atgagctcag agactggccc agtggctgtg gaccccacat
      481 tgagacggcg gatcgagccc catgagtttg aggtattctt cgatccgaga gagctccgca
      541 aggagacctg cctgctttac gaaattaatt gggggggccg gcactccatt tggcgacata
      601 catcacagaa cactaacaag cacgtcgaag tcaacttcat cgagaagttc acgacagaaa
      661 gatatttctg tccgaacaca aggtgcagca ttacctggtt tctcagctac agcccatgcg
      721 gcgaatgtag tagggccatc actgaattcc tgtcaaggta tccccacgtc actctgttta
      781 tttacatcgc aaggctgtac caccacgctg accccgaaaa tcgacaaggc ctgcgggatt
      841 tgatctcttc aggtgtgact atccaaatta tgactgagca ggagtcagga tactgctgga
      901 gaaactttgt gaattatagc ccgagtaatg aagcccactg gcctaggtat ccccatctgt
      961 gggtacgact gtacgttctt gaactgtact gcatcatact gggcctgcct ccttgtctca
     1021 acattctgag aaggaagcag ccacagctga cattctttac catcgctctt cagtcttgtc
     1081 attaccagcg actgccccca cacattctct gggccaccgg gttgaaaagc ggcagcgaga
     1141 ctcccgggac ctcagagtcc gccacacccg aaagtatgtc aaagctggag aaattcacca
     1201 actgttatag cctgtctaag accctgcgct tcaaggcaat cccagtgggc aagacacaag
     1261 agaacattga caacaaacgg ctcctggtgg aggatgagaa gagggctgaa gattacaagg
     1321 gcgttaagaa gctgctggat aggtactatc tgtcattcat caacgatgtc ctccacagta
     1381 tcaagctgaa gaatctgaac aattacattt ctctgttccg gaagaagaca cggaccgaga
     1441 aggagaacaa agagctggag aatctggaga tcaacctgag gaaagaaata gctaaggctt
     1501 tcaaagggaa cgagggttac aagtccctgt tcaagaaaga cattatcgag actattctgc
     1561 ctgagttcct ggacgataaa gatgagatcg ccctcgtcaa ttccttcaat gggtttacca
     1621 cagcctttac cggcttcttc gacaatagag agaatatgtt ctctgaagag gccaaatcca
     1681 ctagcatcgc ctttcgctgc ataaacgaga acctgactag gtacatcagc aatatggaca
     1741 tctttgagaa agtcgatgcc atattcgaca aacatgaggt gcaggagatt aaggagaaga
     1801 tcctgaactc agattacgat gtcgaagatt tcttcgaggg agagttcttc aacttcgtgc
     1861 tcacacaaga gggcattgat gtgtacaatg caatcattgg agggttcgtg acagagagtg
     1921 gcgagaagat aaagggcctg aacgagtata tcaacctcta caaccagaaa accaagcaga
     1981 aactgcctaa gttcaagcca ctgtacaaac aagtgctctc agatagggaa agcctgagct
     2041 tctacggtga agggtataca tcagatgaag aagtgctcga agtgttccgc aacaccctca
     2101 ataagaacag tgaaatcttc tcttcaatca agaagctgga gaaactgttc aagaatttcg
     2161 atgagtactc ctctgccgga atctttgtga agaatggccc tgcaatatcc actattagca
     2221 aagacatctt tggcgagtgg aacgttatca gggataagtg gaatgccgag tacgatgata
     2281 ttcatctcaa gaagaaagcc gtggttacag agaaatacga ggatgataga cgcaagagct
     2341 ttaagaagat tggtagcttc tctctcgaac agctgcagga gtacgccgac gctgacctgt
     2401 cagtcgtgga gaaactcaag gagatcataa tccagaaggt ggatgaaatc tacaaagtgt
     2461 atggaagctc tgagaaactc ttcgatgcag actttgttct ggagaagagt ctgaagaaga
     2521 acgacgcagt ggttgctatc atgaaggacc tgctggattc tgttaagtct ttcgagaatt
     2581 acattaaggc attctttggt gaagggaagg agacaaatag ggacgagagc ttctatggcg
     2641 actttgttct ggcctacgac atcctcctca aggttgacca catctatgac gctatacgga
     2701 attacgttac ccagaagccc tatagcaaag acaagttcaa gctgtatttc cagaatccac
     2761 agtttatggg tgggtgggat aaagacaaag aaacagatta cagggccact atcctgcggt
     2821 acggcagcaa atactatctg gctatcatgg ataagaagta cgccaaatgc ctccagaaga
     2881 tcgacaagga cgacgtgaac ggtaactacg agaagatcaa ttacaagctc ctgccaggac
     2941 ctaacaagat gctgcccaag gtgttcttct ccaagaaatg gatggcctac tataacccaa
     3001 gcgaggacat tcagaagata tacaagaatg ggacattcaa gaagggcgat atgttcaacc
     3061 tcaacgactg ccacaagctg attgatttct tcaaggatag catttctcgc tatcccaagt
     3121 ggtctaatgc atacgatttc aacttcagcg agactgagaa gtacaaagac atcgctggct
     3181 tctaccggga ggtggaagag caaggctata aggtgtcatt cgaatccgct tctaagaagg
     3241 aagtggataa gctcgtggaa gagggtaagc tgtacatgtt ccagatatac aacaaagact
     3301 tcagcgataa gagccacggc actccaaacc tccatactat gtatttcaag ctgctgtttg
     3361 acgagaacaa ccacggacag attaggctgt caggaggcgc agaactcttc atgcgcagag
     3421 cttcactgaa gaaggaggaa ctcgttgtcc acccagccaa tagccctata gccaataaga
     3481 atccagacaa tcctaagaaa accactactc tgtcttacga tgtgtataag gataagagat
     3541 tctctgaaga tcagtacgaa ctgcacatac ccattgccat taacaagtgc cctaagaaca
     3601 tcttcaagat taacacagag gttagagtgc tcctgaaaca cgacgataac ccttatgtta
     3661 taggcattgc tcgcggagag agaaacctgc tgtacatcgt cgtggtggac ggcaaaggca
     3721 acatcgtgga acagtacagt ctcaatgaaa tcattaacaa tttcaacgga atccgcatta
     3781 agaccgacta ccattctctc ctcgacaaga aggagaaaga aaggttcgaa gcaagacaga
     3841 attggacaag tatagagaat atcaaagaac tgaaggctgg gtacatctct caggttgtgc
     3901 acaagatatg tgagctggtg gagaagtacg acgctgttat cgccctcgcg gacctgaata
     3961 gcggcttcaa gaactccagg gtgaaggtgg agaagcaggt gtatcagaag ttcgagaaga
     4021 tgctgatcga caagctcaac tatatggtgg acaagaaatc caatccttgc gctactggtg
     4081 gagccctgaa gggctatcaa atcaccaata agttcgaatc tttcaagtct atgagcaccc
     4141 agaatggctt catcttctac atacccgcat ggctgacatc caagattgat ccctctaccg
     4201 gatttgttaa tctgctcaag actaagtaca cctctattgc tgactcaaag aagttcatat
     4261 catcatttga ccgcatcatg tacgtgccag aagaggacct gttcgagttt gccctggatt
     4321 acaagaattt ctctcggact gacgccgact acatcaagaa gtggaagctc tactcttatg
     4381 gtaatcggat tcgcatattc cgcaatccca agaagaataa cgtgttcgat tgggaggaag
     4441 tttgcctcac cagcgcttac aaggagctgt tcaataagta tgggattaac taccagcagg
     4501 gcgacataag agccctgctg tgcgaacaat ctgataaggc attctattcc tctttcatgg
     4561 cactgatgtc actgatgctg caaatgcgca attccatcac cggaagaaca gacgtggcct
     4621 ttctgatctc tcctgtcaag aactcagatg gcatcttcta cgattcccgc aactatgaag
     4681 cacaggagaa tgctatcctg cctaagaatg ccgatgcaaa tggagcctat aacatcgcca
     4741 gaaaggtcct ctgggccata ggacaattca agaaagctga agatgagaag ctggacaagg
     4801 tgaagatcgc catttcaaac aaagagtggc tcgaatatgc tcagacctca gtgaagcatg
     4861 gatcacccaa gaagaaacgg aaagtgtctg gtggttctac taatctgtca gatattattg
     4921 aaaaggagac cggtaagcaa ctggttatcc aggaatccat cctcatgctc ccagaggagg
     4981 tggaagaagt cattgggaac aagccggaaa gcgatatact cgtgcacacc gcctacgacg
     5041 agagcaccga cgagaatgtc atgcttctga ctagcgacgc ccctgaatac aagccttggg
     5101 ctctggtcat acaggatagc aacggtgaga acaagattaa gatgctctct ggtggttctc
     5161 ccaagaagaa gaggaaagtc gggcccgagg gcagaggaag tctgctaaca tgcggtgacg
     5221 tcgaggagaa tcctggccca actaatctgt cagatattat tgaaaaggag accggtaagc
     5281 aactggttat ccaggaatcc atcctcatgc tcccagagga ggtggaagaa gtcattggga
     5341 acaagccgga aagcgatata ctcgtgcaca ccgcctacga cgagagcacc gacgagaatg
     5401 tcatgcttct gactagcgac gcccctgaat acaagccttg ggctctggtc atacaggata
     5461 gcaacggtga gaacaagatt aagatgctcc ccaagaagaa gaggaaagtc gctactaact
     5521 tcagcctgct gaagcaggct ggagacgtgg aggagaaccc tggacctacc aacctgtccg
     5581 atatcattga gaaagagacc ggcaaacagc tggtgatcca ggagagcatc ctgatgctgc
     5641 ccgaagaggt ggaggaagtg atcggcaaca agcccgagtc cgacatcctg gtgcacacag
     5701 cctatgatga atccaccgac gagaacgtga tgctgctgac ctccgatgct cccgagtata
     5761 aaccctgggc actggtgatc caggactcta atggagagaa caagatcaag atgctgccca
     5821 agaagaagag gaaagtcgaa ggacggggct ccctcctgac ctgtggcgat gtggaagaga
     5881 accccggccc cacaaacctc agtgacatta tcgagaagga aacaggaaaa cagctcgtca
     5941 ttcaagaatc tattcttatg ttgcctgagg aagtcgaaga ggttattggc aataaacctg
     6001 aatctgatat tcttgtccat accgcttacg atgagtccac agatgaaaat gttatgctgc
     6061 tcacatctga cgcaccagag tacaaaccat gggcgctcgt tattcaagat tccaacggcg
     6121 aaaacaaaat caaaatgctt tctggtggtt ctcctaaaaa aaaaagaaag gtgtgagttt
     6181 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     6241 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     6301 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     6361 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     6421 ctatggcttc tgaggcggaa agaaccagct ggggctcgat accgtcgacc tctagctaga
     6481 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     6541 cacacaacat acgagccgga agcataaagt gtaaagccta gggtgcctaa tgagtgagct
     6601 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     6661 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     6721 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     6781 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     6841 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     6901 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     6961 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     7021 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     7081 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     7141 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     7201 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     7261 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     7321 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     7381 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     7441 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     7501 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     7561 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     7621 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     7681 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     7741 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     7801 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     7861 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     7921 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     7981 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     8041 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     8101 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     8161 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     8221 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     8281 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     8341 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     8401 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     8461 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     8521 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     8581 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     8641 tgccacctga cgtcgacgga tcgggagatc gatctcccga tcccctaggg tcgactctca
     8701 gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg
     8761 aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca
     8821 attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca
     8881 gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
     8941 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     9001 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     9061 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     9121 tggcagtaca tca
//
