LOCUS       sequence-212124-        8917 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EF1-CBR2opt-T2A-copGFP (construct used for lentiviral transduction).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8917)
  AUTHORS   Hall MP, Woodroofe CC, Wood MG, Que I, Van't Root M, Ridwan Y, Shi 
            C, Kirkland TA, Encell LP, Wood KV, Lowik C, Mezzanotte L
  TITLE     Click beetle luciferase mutant and near infrared naphthyl-luciferins
            for improved bioluminescence imaging.
  JOURNAL   Nat Commun. 2018 Jan 9;9(1):132. doi: 10.1038/s41467-017-02542-9.
  PUBMED    29317625
REFERENCE   2  (bases 1 to 8917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-01-9"; volume: "9(1):132. doi"; pages: " 
            10.1038/s41467-017-02542-9"
COMMENT     Sequence Label: ATG-2460
FEATURES             Location/Qualifiers
     source          1..8917
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..232
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             233..413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1078..1311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1496..1540
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1807..1923
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1966..2177
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2190..2458
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      2515..2524
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4159..4212
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4243..4905
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     misc_feature    4996..5584
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5049..5069)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5467..5478)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5658..5891
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5963..6084
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6000..6019)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6054..6073
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6124..6259
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6292..6308)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6292..6308)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6305..6327)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6316..6332
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6340..6346,6347..6364,6365..6370))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6385..6406
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6523..6540)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6694..7282)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(7453..8244,8245..8313))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8076..8095
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8314..8418)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8486..8504
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8542..8564)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8664..8683
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8877..8899
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8891..8908
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8892..8908
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat
     1921 tttatcgata ctagtggatc tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat
     1981 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa
     2041 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
     2101 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
     2161 ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca cgcgcccgcc
     2221 gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg
     2281 gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg agaccgggcc
     2341 tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga
     2401 ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcca
     2461 agctgtgacc ggcgcctact ctagagctag cgaattcgaa tttaaatcgg atccgccacc
     2521 atggttaaga gagaaaaaaa tgttatttat ggtcctgaac ctctgcatcc tctggaagat
     2581 ctcactgcag gagagatgct gtttcgcgcc ctgcgcaagc actctcactt gccgcaggct
     2641 ctggtcgacg tggtgggtga cgagtccctg agctacaagg agttcttcga ggcgactgtc
     2701 ctgctggcac agagtctcca taactgcggc tacaagatga acgatgtggt ttccatctgc
     2761 gcagagaaca acactagatt ctttatccca gtgatcgctg cttggtacat cggcatgatt
     2821 gtcgcaccag tcaatgaatc ttacattcca gatgaactgt gtaaggttat gggaatttcc
     2881 aaaccgcaga tcgtgttcac aactaagaac atcctgaaca aggttctgga agtgcagagt
     2941 cggaccaatt tcatcaagcg tattatcatc ctggacacag tggagaacat ccacggttgc
     3001 gaatccctcc ccaacttcat ctctcgttat agcgatggca acatcgctaa tttcaaaccc
     3061 ctgcatttcg atcctgttga acaggttgcc gccatcctgt gctcctctgg gaccaccgga
     3121 ctgccgaaag gcgtgatgca gacccatcag aacatctgcg tgaggctgat ccacgccctg
     3181 gacccacgct atggaactca gctgatccca ggagtcactg tgctggttta cctccctttt
     3241 tttcacgcct tcggtttcca cattaccctc gggtacttta tggtcggcct ccgcgttatc
     3301 atgttccgtc gctttgacca ggaggctttc ctgaaggcca tccaggatta tgaggtgagg
     3361 agtgtgatta acgtcccaag cgtgatcctg ttcctgtcta agtcccctct ggttgacaag
     3421 tacgatctga gctccctgcg tgagctgtgc tgcggggctg caccactggc caaagaagtc
     3481 gcggaagtgg ctgccaaacg cctgaacctg ccgggtatct cctgtggctt cggcctgaca
     3541 gagagcacat ccgctatcat ccagacactg cgggatgagt ttaagtccgg ttccctgggc
     3601 cgggttacac ccctgatggc agccaagatc gccgaccgcg agactgggaa ggccctcgga
     3661 cctaaccagg tcggagagct gtgtattaag ggccccatgg tgtccaaggg atatgtgaac
     3721 aacgtggaag ctacaaagga ggcgattgat gatgacggat ggctccatag cggagacttt
     3781 gggtactatg acgaggacga gcacttctat gtggtcgaca ggtacaagga gctgatcaag
     3841 tacaagggct cccaggtggc cccagctgaa ctggaggaga ttctgttgaa gaacccttgt
     3901 atcagagatg tggctgttgt ggggattcct gacctcgaag caggtgagct gccctccgct
     3961 ttcgtggtga agcagcctgg tactgagatc accgccaaag aggtgtacga ttacctggct
     4021 gagcgcgtgt cccacaccaa atacctccgc ggcggggtcc gtttcgtgga ctccattccc
     4081 aggaacgtga ccggcaagat cacccgcaag gagctgctga agcagctgct ggttaaggct
     4141 ggcggcgttg cggccgctga gggcagagga agtcttctaa catgcggtga cgtggaggag
     4201 aatcccggcc cttccggaat ggagagcgac gagagcggcc tgcccgccat ggagatcgag
     4261 tgccgcatca ccggcaccct gaacggcgtg gagttcgagc tggtgggcgg cggagagggc
     4321 acccccaagc agggccgcat gaccaacaag atgaagagca ccaaaggcgc cctgaccttc
     4381 agcccctacc tgctgagcca cgtgatgggc tacggcttct accacttcgg cacctacccc
     4441 agcggctacg agaacccctt cctgcacgcc atcaacaacg gcggctacac caacacccgc
     4501 atcgagaagt acgaggacgg cggcgtgctg cacgtgagct tcagctaccg ctacgaggcc
     4561 ggccgcgtga tcggcgactt caaggtggtg ggcaccggct tccccgagga cagcgtgatc
     4621 ttcaccgaca agatcatccg cagcaacgcc accgtggagc acctgcaccc catgggcgat
     4681 aacgtgctgg tgggcagctt cgcccgcacc ttcagcctgc gcgacggcgg ctactacagc
     4741 ttcgtggtgg acagccacat gcacttcaag agcgccatcc accccagcat cctgcagaac
     4801 gggggcccca tgttcgcctt ccgccgcgtg gaggagctgc acagcaacac cgagctgggc
     4861 atcgtggagt accagcacgc cttcaagacc cccatcgcct tcgccagatc ccgcgctcag
     4921 tcgtccaatt ctgccgtgga cggcaccgcc ggacccggct ccaccggatc tcgctagagc
     4981 tgaatctaag tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     5041 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     5101 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     5161 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     5221 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     5281 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     5341 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     5401 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     5461 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     5521 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     5581 ccgcctggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     5641 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagataag atctgctttt
     5701 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     5761 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     5821 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     5881 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     5941 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
     6001 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     6061 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
     6121 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     6181 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     6241 ggaggctttt ttggaggcct agacttttgc agagaccaaa ttcgtaatca tgtcatagct
     6301 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     6361 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     6421 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     6481 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     6541 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     6601 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     6661 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     6721 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     6781 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     6841 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     6901 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     6961 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     7021 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     7081 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     7141 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     7201 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     7261 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     7321 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     7381 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     7441 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     7501 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     7561 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     7621 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     7681 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     7741 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     7801 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     7861 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     7921 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     7981 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     8041 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     8101 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     8161 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     8221 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     8281 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     8341 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     8401 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
     8461 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg
     8521 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     8581 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     8641 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
     8701 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc
     8761 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
     8821 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
     8881 gtcacgacgt tgtaaaacga cggccagtgc caagctg
//
