LOCUS       sequence-21213-e         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Cujec TP, Okamoto H, Fujinaga K, Meyer J, Chamberlin H, Morgan DO, 
            Peterlin BM
  TITLE     The HIV transactivator TAT binds to the CDK-activating kinase and 
            activates the phosphorylation of the carboxy-terminal domain of RNA 
            polymerase II.
  JOURNAL   Genes Dev. 1997 Oct 15. 11(20):2645-57.
  PUBMED    9334327
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            1997 Oct 15. 11(20):2645-57."
COMMENT     Sequence Label: pcDNA3 Tat HA (P#29)
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(28..54)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      354..363
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(394..413)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(395..413)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(458..661)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(487..507)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tgatggatat ctgcagaatt ctcaggcagc gtaatctgga acatcgtatg ggtaatcgaa
       61 cggatctgtc tctgtctctc tctccacctt cttcttcgat tccgtgggcc ctgtcgggtc
      121 ccctcgggac tgggaggcgg gttgctttga tagagaagct tgatgagtct gactgtcctg
      181 aggagctctt cgtcgctgtc tccgcttctt cctgccatag gagatgccta agccttttct
      241 tgtgaaacac gcgtagcaat gaaagcaaca ctttttacaa tagcaattgt tacaagcagt
      301 cctaggctga cttcctggat gcttccaggg ctctagatta ggatctactg gctccatggt
      361 ggcggatccg agctcggtac caagcttggg tctccctata gtgagtcgta ttaatttcga
      421 taagccagta agcagtgggt tctctagtta gccagagagc tctgcttata tagacctccc
      481 accgtacacg cctaccgccc atttgcgtca atggggcgga gttgttacga cattttggaa
      541 agtcccgttg attttggtgc caaaacaaac tcccattgac gtcaatgggg tggagacttg
      601 gaaatccccg tgagtcaaac cgctatccac gcccattgat gtactgccaa aaccgcatca
      661 ccatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccataagg
      721 tcatgtactg ggcataatgc caggcgg
//
