LOCUS       sequence-212139-        1836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Two SpyCatchers linked by an elastin-like polypeptide with an RGDSP 
            integrin-binding site and a C-terminal SnoopCatcher.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Wieduwild R, Howarth M
  TITLE     Assembling and decorating hyaluronan hydrogels with twin protein 
            superglues to mimic cell-cell interactions.
  JOURNAL   Biomaterials. 2018 Oct;180:253-264. doi: 
            10.1016/j.biomaterials.2018.07.020. Epub 2018 Jul 17.
  PUBMED    30053659
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biomaterials."; date: "2018-10"; volume: "180:253-264. doi"; pages:
            " 10.1016/j.biomaterials.2018.07.020. Epub 2018 Jul 17"
COMMENT     Sequence Label: pQE80L TriCatcher-RGDSP
FEATURES             Location/Qualifiers
     source          1..1836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..33
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             52..72
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             79..417
                     /codon_start=1
                     /product="truncated version of the CnaB2 adhesin domain of 
                     Streptococcus pyogenes FbaB"
                     /label=SpyCatcher
                     /note="forms a covalent isopeptide bond with the SpyTag 
                     peptide (Zakeri et al., 2012)"
                     /translation="VDTLSGLSSEQGQSGDMTIEEDSATHIKFSKRDEDGKELAGATME
                     LRDSSGKTISTWISDGQVKDFYLYPGKYTFVETAAPDGYEVATAITFTVNEQGQVTVNG
                     KATKGDAHI"
     CDS             1012..1032
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1039..1377
                     /codon_start=1
                     /product="truncated version of the CnaB2 adhesin domain of 
                     Streptococcus pyogenes FbaB"
                     /label=SpyCatcher
                     /note="forms a covalent isopeptide bond with the SpyTag 
                     peptide (Zakeri et al., 2012)"
                     /translation="VDTLSGLSSEQGQSGDMTIEEDSATHIKFSKRDEDGKELAGATME
                     LRDSSGKTISTWISDGQVKDFYLYPGKYTFVETAAPDGYEVATAITFTVNEQGQVTVNG
                     KATKGDAHI"
     CDS             1498..1833
                     /label=SnoopCatcher
ORIGIN
        1 atgaaaggca gcagccatca tcatcatcat cacgtcgaca tcccaacgac cgaaaacctg
       61 tattttcagg gcgccatggt tgatacctta tcaggtttat caagtgagca aggtcagtcc
      121 ggtgatatga caattgaaga agatagtgct acccatatta aattctcaaa acgtgatgag
      181 gacggcaaag agttagctgg tgcaactatg gagttgcgtg attcatctgg taaaactatt
      241 agtacatgga tttcagatgg acaagtgaaa gatttctacc tgtatccagg aaaatataca
      301 tttgtcgaaa ccgcagcacc agacggttat gaggtagcaa ctgctattac ctttacagtt
      361 aatgagcaag gtcaggttac tgtaaatggc aaagcaacta aaggtgacgc tcatattgac
      421 ggtagcggtg gctctggcgg tcagctcgac ggccacggcg tgggtgttcc gggcgtcggt
      481 gtgccgggtg tgggtgtgcc gggcgaaggt gtgccgggcg tcggtgtgcc gggtgttggt
      541 gttccgggcg ttggtgtgcc gggcgttggc gtgccgggcg agggtgtgcc gggcgttggt
      601 gttccgggcg tgggtgtgcc gggcgtgggc gtgccgggcg tcggtgttcc gggcgagggt
      661 gtgccgggcg taggtgtgcc gggcgttggt gagctctatg cggttaccgg ccgtggtgat
      721 agtccggcca gctctgcccc gatcgccact agtgtgccgg gcgtcggcgt gccgggcgta
      781 ggtgttccgg gcgagggtgt tccgggcgtt ggtgtgccgg gcgtcggcgt gccgggcgtg
      841 ggtgttccgg gcgtaggtgt gccgggcgag ggtgtgccgg gcgtgggcgt gccgggcgta
      901 ggtgttccgg gcgtaggtgt tccgggcgta ggtgttccgg gtgaaggcgt gccgggcgtt
      961 ggtgtgccgg gtgtgggcgt gccgggcggg ctgctcgaca tcccaacgac cgaaaacctg
     1021 tattttcagg gcgccatggt tgatacctta tcaggtttat caagtgagca aggtcagtcc
     1081 ggtgatatga caattgaaga agatagtgct acccatatta aattctcaaa acgtgatgag
     1141 gacggcaaag agttagctgg tgcaactatg gagttgcgtg attcatctgg taaaactatt
     1201 agtacatgga tttcagatgg acaagtgaaa gatttctacc tgtatccagg aaaatataca
     1261 tttgtcgaaa ccgcagcacc agacggttat gaggtagcaa ctgctattac ctttacagtt
     1321 aatgagcaag gtcaggttac tgtaaatggc aaagcaacta aaggtgacgc tcatattgga
     1381 tcccccgcca acctgaaggc cctggaggcc cagaagcaga aggagcagag acaggccgcc
     1441 gaggagctgg ccaacgccaa gaagctgaag gagcagctgg agaagggatc ccatatgaag
     1501 ccgctgcgtg gtgccgtgtt tagcctgcag aaacagcatc ccgactatcc cgatatctat
     1561 ggcgcgattg atcagaatgg gacctatcaa aatgtgcgta ccggcgaaga tggtaaactg
     1621 acctttaaga atctgagcga tggcaaatat cgcctgtttg aaaatagcga acccgctggc
     1681 tataaaccgg tgcagaataa gccgattgtg gcgtttcaga ttgtgaatgg cgaagtgcgt
     1741 gatgtgacca gcattgtgcc gcaggatatt ccggctacat atgaatttac caacggtaaa
     1801 cattatatca ccaatgaacc gataccgccg aaataa
//
