LOCUS       sequence-21214-e         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Cujec TP, Okamoto H, Fujinaga K, Meyer J, Chamberlin H, Morgan DO, 
            Peterlin BM
  TITLE     The HIV transactivator TAT binds to the CDK-activating kinase and 
            activates the phosphorylation of the carboxy-terminal domain of RNA 
            polymerase II.
  JOURNAL   Genes Dev. 1997 Oct 15. 11(20):2645-57.
  PUBMED    9334327
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            1997 Oct 15. 11(20):2645-57."
COMMENT     Sequence Label: pcDNA3 Tat K41A HA (P#37)
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      103..112
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             412..438
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(505..523)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(506..523)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(543..560)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    549..771
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gnngagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gcttggtacc gagctcggat ccgccaccat ggagccagta
      121 gatcctaatc tagagccctg gaagcatcca ggaagtcagc ctaggactgc ttgtaacaat
      181 tgctattgta aaaagtgttg ctttcattgc tacgcgtgtt tcacaagagc cggcttaggc
      241 atctcctatg gcaggaagaa gcggagacag cgacgaagag ctcctcagga cagtcagact
      301 catcaagctt ctctatcaaa gcaacccgcc tcccagtccc gaggggaccc gacagggccc
      361 acggaatcga agaagaaggt ggagagagag acagagacag atccgttcga ttacccatac
      421 gatgttccag attacgctgc ctgagaattc tgcagatatc catcacactg gcggccgctc
      481 gagcatgcat ctagagggcc ctattctata gtgtcaccta aatgctagag ctcgctgatc
      541 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
      601 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
      661 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
      721 ggaggattgg ganacatagc nngcatgctg gggatgcggt gggctctatg gcttctgngg
      781 nggaaagaan cagctggggn nnnnggggg
//
