LOCUS       sequence-21215-e         603 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Cujec TP, Cho H, Maldonado E, Meyer J, Reinberg D, Peterlin BM
  TITLE     The human immunodeficiency virus transactivator Tat interacts with 
            the RNA polymerase II holoenzyme.
  JOURNAL   Mol Cell Biol. 1997 Apr . 17(4):1817-23.
  PUBMED    9121429
REFERENCE   2  (bases 1 to 603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 1997 Apr . 17(4):1817-23."
COMMENT     Sequence Label: pcDNAI Tat C30G HA (P#38)
FEATURES             Location/Qualifiers
     source          1..603
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(47..73)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      373..382
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(413..432)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(414..432)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(477..576)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(506..526)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gctcgagcgg ccgccagtgt gatggatatc tgcagaattc tcaggcagcg taatctggaa
       61 catcgtatgg gtaatcgaac ggatctgtct ctgtctctct ctccaccttc ttcttcgatt
      121 ccgtgggccc tgtcgggtcc cctcgggact gggaggcggg ttgctttgat agagaagctt
      181 gatgagtctg actgtcctga ggagctcttc gtcgctgtct ccgcttcttc ctgccatagg
      241 agatgcctaa gccttttctt gtgaaacacg cgtacccatg aaagcagccc tttttacaat
      301 agcaattgtt acaagcagtc ctaggctgac ttcctggatg cttccagggc tctagattag
      361 gatctactgg ctccatggtg gcggatccga gctcggtacc aagcttgggt ctccctatag
      421 tgagtcgtat taatttcgat aagccagtaa gcagtgggtt ctctagttag ccagagagct
      481 ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag
      541 ttgttacgac attttggaaa gtcccgttga ttttggtgcc aaaacaaact cccattgacg
      601 tca
//
